TB Genome Annotation Portal

Rv2919c (glnB)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: nitrogen regulatory protein P-II
  • REFSEQ: nitrogen regulatory protein P-II GLNB
  • PATRIC: Nitrogen regulatory protein P-II
  • TUBERCULIST: Probable nitrogen regulatory protein P-II GlnB
  • NCBI: Probable nitrogen regulatory protein P-II GlnB
  • updated information (H37Rv4):
    • gene name: glnK
    • function:
    • reference: Shetty (2010) PMID: 20521335
• Type: Not Target
• Start: 3230738
• End: 3231076
• Operon:


• Trans-membrane region:
  
• Role: I.J.1 - Repressors/activators
• GO terms:
  • GO:0050790 - regulation of catalytic activity (Uniprot)
  • GO:0044119 - growth of symbiont in host cell (Uniprot)
  • GO:0043531 - ADP binding (Uniprot)
  • GO:0030234 - enzyme regulator activity (Uniprot)
  • GO:0006808 - regulation of nitrogen utilization (Uniprot)
  • GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
  • GO:0006351 - transcription, DNA-templated (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 3lf0, 3bzq
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3LF0	100%	Mycobacterium tuberculosis	Crystal structure of the ATP bound Mycobacterium tuberculosis nitrogen regulatory PII protein
  3BZQ	100%	Mycobacterium tuberculosis H37Rv	High resolution crystal structure of Nitrogen Regulatory Protein (Rv2919c) of Mycobacterium tuberculosis
  1HWU	64%	Herbaspirillum seropedicae	STRUCTURE OF PII PROTEIN FROM HERBASPIRILLUM SEROPEDICAE
  5L9N	61%	Escherichia coli (strain K12)	Structure of uridylylated GlnB from Escherichia coli bound to ATP
  2PII	61%	Escherichia coli	PII, GLNB PRODUCT
  1QY7	61%	Synechococcus elongatus	The structure of the PII protein from the cyanobacteria Synechococcus sp. PCC 7942
  1PIL	61%	Escherichia coli	STRUCTURE OF THE ESCHERICHIA COLI SIGNAL TRANSDUCING PROTEIN PII
  4C3M	60%	SYNECHOCOCCUS ELONGATUS	Structure of wildtype PII from S. elongatus at medium resolution
  4C3L	60%	SYNECHOCOCCUS ELONGATUS	Structure of wildtype PII from S. elongatus at high resolution
  4C3K	60%	SYNECHOCOCCUS ELONGATUS	Structure of mixed PII-ADP complexes from S. elongatus

• Links to additional information on glnB:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -0.689 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MKLITAIVKPFTLDDVKTSLEDAGVLGMTVSEIQGYGRQKGHTEVYRGAEYSVDFVPKVRIEVVVDDSIVDKVVDSIVRAARTGKIGDGKVWVSPVDTIV
RVRTGERGHDAL

Additional Information