TB Genome Annotation Portal

Rv1801 (PPE29)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: PPE family protein
  • REFSEQ: PPE family protein
  • PATRIC: PPE family protein
  • TUBERCULIST: PPE family protein PPE29
  • NCBI: PPE family protein PPE29
  • updated information (H37Rv4):
    • gene name: PPE29
    • function:
    • reference:
• Type: Not Target
• Start: 2042001
• End: 2043272
• Operon:


• Trans-membrane region:
  
• Role: IV.C.2 - PPE family
• GO terms:
  • GO:0005618 - cell wall (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5XFS	60%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal structure of PE8-PPE15 in complex with EspG5 from M. tuberculosis
  6VHR	45%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Structure of PE5-PPE4-EspG3 complex from the type VII (ESX-3) secretion system, space group I422
  6UUJ	45%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Structure of PE5-PPE4-EspG3 complex from the type VII (ESX-3) secretion system, space group P212121
  4W4L	36%	Mycobacterium tuberculosis	Crystal structure of EspG5 in complex with PE25 and PPE41 from the ESX-5 type VII secretion system of M. tuberculosis
  4W4K	36%	Mycobacterium tuberculosis	Crystal structure of a PE25-PPE41 heterodimer from a type VII secretion system of M. tuberculosis
  4KXR	36%	Mycobacterium tuberculosis	Structure of the Mycobacterium tuberculosis type VII secretion system chaperone EspG5 in complex with PE25-PPE41 dimer
  2G38	36%	Mycobacterium tuberculosis	A PE/PPE Protein Complex from Mycobacterium tuberculosis

• Links to additional information on PPE29:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.876 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MDFGLLPPEINSGRMYTGPGPGPMLAAATAWDGLAVELHATAAGYASELSALTGAWSGPSSTSMASAAAPYVAWMSATAVHAELAGAQARLAIAAYEAAF
AATVPPPVIAANRAQLMVLIATNIFGQNTPAIMMTEAQYMEMWAQDAAAMYGYAGSSATASRMTAFTEPPQTTNHGQLGAQSSAVAQTAATAAGGNLQSA
FPQLLSAVPRALQGLALPTASQSASATPQWVTDLGNLSTFLGGAVTGPYTFPGVLPPSGVPYLLGIQSVLVTQNGQGVSALLGKIGGKPITGALAPLAEF
ALHTPILGSEGLGGGSVSAGIGRAGLVGKLSVPQGWTVAAPEIPSPAAALQATRLAAAPIAATDGAGALLGGMALSGLAGRAAAGSTGHPIGSAAAPAVG
AAAAAVEDLATEANIFVIPAMDD

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	1.91 (0.44)	1.77 (0.89)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link