TB Genome Annotation Portal

Rv3883c (mycP1)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: type VII secretion-associated serine protease mycosin
  • REFSEQ: membrane-anchored mycosin MYCP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1)
  • PATRIC: serine protease
  • TUBERCULIST: Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1)
  • NCBI: Membrane-anchored mycosin MycP1 (serine protease) (subtilisin-like protease) (subtilase-like) (mycosin-1)
  • updated information (H37Rv4):
    • gene name: mycP1
    • function:
    • reference:
• Type: Not Target
• Start: 4363417
• End: 4364757
• Operon:


• Trans-membrane region:
  
• Role: II.B.3 - Proteins, peptides and glycopeptides
• GO terms:
  • GO:0051046 - regulation of secretion (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0009986 - cell surface (Uniprot)
  • GO:0009405 - pathogenesis (Uniprot)
  • GO:0008236 - serine-type peptidase activity (Uniprot)
  • GO:0008233 - peptidase activity (Uniprot)
  • GO:0006508 - proteolysis (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0004252 - serine-type endopeptidase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4HVL	75%	Mycobacterium thermoresistibile	Structure of a serine protease MycP1, an essential component of the type VII (ESX-1) secretion system
  4M1Z	75%	Mycobacterium smegmatis	Crystal structure of MycP1 with the N-terminal propeptide removed
  4KPG	74%	Mycobacterium smegmatis	Crystal structure of MycP1 from the ESX-1 type VII secretion system
  4KB5	74%	Mycobacterium smegmatis	Crystal structure of MycP1 from Mycobacterium smegmatis
  4J94	74%	Mycobacterium smegmatis	Crystal structure of MycP1 from the ESX-1 type VII secretion system
  4KG7	49%	Mycobacterium smegmatis	Structure of MycP3 protease from the type VII (ESX-3) secretion system.

• Links to additional information on mycP1:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.319 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VHRIFLITVALALLTASPASAITPPPIDPGALPPDVTGPDQPTEQRVLCASPTTLPGSGFHDPPWSNTYLGVADAHKFATGAGVTVAVIDTGVDASPRVP
AEPGGDFVDQAGNGLSDCDAHGTLTASIIAGRPAPTDGFVGVAPDARLLSLRQTSEAFEPVGSQANPNDPNATPAAGSIRSLARAVVHAANLGVGVINIS
EAACYKVSRPIDETSLGASIDYAVNVKGVVVVVAAGNTGGDCVQNPAPDPSTPGDPRGWNNVQTVVTPAWYAPLVLSVGGIGQTGMPSSFSMHGPWVDVA
APAENIVALGDTGEPVNALQGREGPVPIAGTSFAAAYVSGLAALLRQRFPDLTPAQIIHRITATARHPGGGVDDLVGAGVIDAVAALTWDIPPGPASAPY
NVRRLPPPVVEPGPDRRPITAVALVAVGLTLALGLGALARRALSRR

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	2.26 (0.63)	2.49 (0.9)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link