TB Genome Annotation Portal

Rv3132c (devS)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: redox sensor histidine kinase response regulator devS
  • REFSEQ: two component sensor histidine kinase DEVS
  • PATRIC: Two component system sensor histidine kinase DevS
  • TUBERCULIST: Two component sensor histidine kinase DevS
  • NCBI: Two component sensor histidine kinase DevS
  • updated information (H37Rv4):
    • gene name: dosS/devS
    • function:
    • reference:
• Type: Not Target
• Start: 3497529
• End: 3499265
• Operon:


• Trans-membrane region:
  
• Role: I.J.2 - Two component systems
• GO terms:
  • GO:0070483 - detection of hypoxia (Uniprot)
  • GO:0070026 - nitric oxide binding (Uniprot)
  • GO:0070025 - carbon monoxide binding (Uniprot)
  • GO:0051776 - detection of redox state (Uniprot)
  • GO:0051775 - response to redox state (Uniprot)
  • GO:0046983 - protein dimerization activity (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0046777 - protein autophosphorylation (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0023014 - signal transduction by protein phosphorylation (Uniprot)
  • GO:0020037 - heme binding (Uniprot)
  • GO:0019826 - oxygen sensor activity (Uniprot)
  • GO:0019825 - oxygen binding (Uniprot)
  • GO:0018106 - peptidyl-histidine phosphorylation (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016310 - phosphorylation (Uniprot)
  • GO:0016301 - kinase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0004673 - protein histidine kinase activity (Uniprot)
  • GO:0004672 - protein kinase activity (Uniprot)
  • GO:0000287 - magnesium ion binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
  • GO:0000160 - phosphorelay signal transduction system (Uniprot)
  • GO:0000155 - phosphorelay sensor kinase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 2w3h, 3zxo, 2w3g, 2w3f, 2w3e, 2w3d, 2y79, 2y8h, 2w3d
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4YOF	100%	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	DosS GAFA Domain Reduced Nitric Oxide Bound Crystal Structure
  4YNR	100%	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	DosS GAFA Domain Reduced CO Bound Crystal Structure
  2W3H	100%	MYCOBACTERIUM TUBERCULOSIS	Cyanide bound structure of the first GAF domain of Mycobacterium tuberculosis DosS
  2W3G	100%	MYCOBACTERIUM TUBERCULOSIS	Air-oxidized structure of the first GAF domain of Mycobacterium tuberculosis DosS
  2W3F	100%	MYCOBACTERIUM TUBERCULOSIS	Reduced structure of the first GAF domain of Mycobacterium tuberculosis DosS
  2W3E	100%	MYCOBACTERIUM TUBERCULOSIS	Oxidized structure of the first GAF domain of Mycobacterium tuberculosis DosS
  2W3D	100%	MYCOBACTERIUM TUBERCULOSIS	Structure of the first GAF domain of Mycobacterium tuberculosis DosS
  2Y8H	99%	MYCOBACTERIUM TUBERCULOSIS	STRUCTURE OF THE FIRST GAF DOMAIN E87G MUTANT OF MYCOBACTERIUM TUBERCULOSIS DOSS
  2Y79	99%	MYCOBACTERIUM TUBERCULOSIS	STRUCTURE OF THE FIRST GAF DOMAIN E87A MUTANT OF MYCOBACTERIUM TUBERCULOSIS DOSS
  3ZXO	96%	MYCOBACTERIUM TUBERCULOSIS	CRYSTAL STRUCTURE OF THE MUTANT ATP-BINDING DOMAIN OF MYCOBACTERIUM TUBERCULOSIS DOSS

• Links to additional information on devS:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.226 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MTTGGLVDENDGAAMRPLRHTLSQLRLHELLVEVQDRVEQIVEGRDRLDGLVEAMLVVTAGLDLEATLRAIVHSATSLVDARYGAMEVHDRQHRVLHFVY
EGIDEETVRRIGHLPKGLGVIGLLIEDPKPLRLDDVSAHPASIGFPPYHPPMRTFLGVPVRVRDESFGTLYLTDKTNGQPFSDDDEVLVQALAAAAGIAV
ANARLYQQAKARQSWIEATRDIATELLSGTEPATVFRLVAAEALKLTAADAALVAVPVDEDMPAADVGELLVIETVGSAVASIVGRTIPVAGAVLREVFV
NGIPRRVDRVDLEGLDELADAGPALLLPLRARGTVAGVVVVLSQGGPGAFTDEQLEMMAAFADQAALAWQLATSQRRMRELDVLTDRDRIARDLHDHVIQ
RLFAIGLALQGAVPHERNPEVQQRLSDVVDDLQDVIQEIRTTIYDLHGASQGITRLRQRIDAAVAQFADSGLRTSVQFVGPLSVVDSALADQAEAVVREA
VSNAVRHAKASTLTVRVKVDDDLCIEVTDNGRGLPDEFTGSGLTNLRQRAEQAGGEFTLASVPGASGTVLRWSAPLSQ

Additional Information

dosS