Rv3112 (moaD1)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: molybdopterin converting factor subunit 1
- REFSEQ: molybdenum cofactor biosynthesis protein D
- PATRIC: Molybdenum cofactor biosynthesis protein MoaD
- TUBERCULIST: Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor subunit 1)
- NCBI: Probable molybdenum cofactor biosynthesis protein D MoaD1 (molybdopterin converting factor small subunit) (molybdopterin [MPT] converting factor, subunit 1)
- updated information (H37Rv4):
  - gene name: moaD1
  - function:
  - reference:
Type: Not Target
Start: 3479700
End: 3479951
Operon:

Trans-membrane region:
Role: I.G.4 - Molybdopterin
GO terms:
- GO:0006777 - Mo-molybdopterin cofactor biosynthetic process (Uniprot)
- GO:0000166 - nucleotide binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on moaD1:
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.271 (from Jeremy Rock's lab)

Amino Acid Sequence

MIKVNVLYFGAVREACDETPREEVEVQNGTDVGNLVDQLQQKYPRLRDHCQRVQMAVNQFIAPLSTVLGDGDEVAFIPQVAGG

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 7 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 7 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 7 sites
Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.998850; 7 non-insertions in a row out of 7 sites
Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.998750; 7 non-insertions in a row out of 7 sites
Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.06
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0081 (-) (Direct binding)
- Rv0324 (-) (Direct binding)
- Rv0691c (-) (Direct binding)
- Rv1985c (-) (Direct binding)
- Rv3597c (lsr2) (Direct binding)
- Rv3736 (-) (Direct binding)
- Rv0047c (-) (Upstream of operon)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Not downregulated by other genes.

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv3112 (moaD1)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	PhysicalInteraction Rv3324c	priti.priety	NAS		Co-occurrence(Functional-linkage) authors,PR. Marri,JP. Bannantine,GB. Golding Comparative genomics of metabolic pathways in Mycobacterium species: gene duplication, gene decay and lateral gene transfer. FEMS Microbiol. Rev. 2006
Interaction	PhysicalInteraction Rv3324A	priti.priety	NAS		Co-occurrence(Functional-linkage) authors,PR. Marri,JP. Bannantine,GB. Golding Comparative genomics of metabolic pathways in Mycobacterium species: gene duplication, gene decay and lateral gene transfer. FEMS Microbiol. Rev. 2006
Interaction	PhysicalInteraction Rv3324A	priti.priety	NAS		Co-occurrence(Functional-linkage) authors,MS. Cortese,AB. Caplan,RL. Crawford Structural, functional, and evolutionary analysis of moeZ, a gene encoding an enzyme required for the synthesis of the Pseudomonas metabolite, pyridine-2,6-bis(thiocarboxylic acid). BMC Evol. Biol. 2002
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,W. Zhang,A. Urban,H. Mihara,S. Leimkhler,T. Kurihara,N. Esaki IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. J. Biol. Chem. 2010
Citation	Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Nature 2001	priyadarshinipriyanka2001	ISS	11713534	Geneneighbourhood
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. Nature 2001
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. Nature 2001
Citation	Revisiting the evolution of Mycobacterium bovis. authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr J. Bacteriol. 2005	priyadarshinipriyanka2001	ISS	16159772	Geneneighbourhood
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr Revisiting the evolution of Mycobacterium bovis. J. Bacteriol. 2005
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr Revisiting the evolution of Mycobacterium bovis. J. Bacteriol. 2005
Citation	IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. authors,W. Zhang,A. Urban,H. Mihara,S. Leimkhler,T. Kurihara,N. Esaki J. Biol. Chem. 2010	priyadarshinipriyanka2001	ISS	19946146	Geneneighbourhood
Interaction	Operon Rv3112	priyadarshinipriyanka2001	ISS		Geneneighbourhood authors,W. Zhang,A. Urban,H. Mihara,S. Leimkhler,T. Kurihara,N. Esaki IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. J. Biol. Chem. 2010
Citation	Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis. authors,TP. Stinear,T. Seemann,PF. Harrison,GA. Jenkin,JK. Davies,PD. Johnson,Z. Abdellah,C. Arrowsmith,T. Chillingworth,C. Churcher,K. Clarke,A. Cronin,P. Davis,I. Goodhead,N. Holroyd,K. Jagels,A. Lord,S. Moule,K. Mungall,H. Norbertczak,MA. Quail,E. Rabbinowitsch,D. Walker,B. White,S. Whitehead,PL. Small,R. Brosch,L. Ramakrishnan,MA. Fischbach,J. Parkhill,ST. Cole Genome Res. 2008	mwilliams		18403782	Acquired by horizontal gene transfer
Citation	High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling. authors,P. Brodin,Y. Poquet,F. Levillain,I. Peguillet,G. Larrouy-Maumus,M. Gilleron,F. Ewann,T. Christophe,D. Fenistein,J. Jang,MS. Jang,SJ. Park,J. Rauzier,JP. Carralot,R. Shrimpton,A. Genovesio,JA. Gonzalo-Asensio,G. Puzo,C. Martin,R. Brosch,GR. Stewart,B. Gicquel,O. Neyrolles PLoS Pathog. 2010	mwilliams		20844580	Transposon mutant in M. tuberculosis W-Beijing strain was defective in arresting phagosome maturation
Citation	Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a fused molybdopterin synthase in Mycobacterium tuberculosis. authors,MJ. Williams,BD. Kana,V. Mizrahi J. Bacteriol. 2011	mwilliams		20971904	Deletion of moaA1-moaB1-moaC1-moaD1 in M. tuberculosis results in reduced MoCo biosynthesis
Citation	Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a fused molybdopterin synthase in Mycobacterium tuberculosis. authors,MJ. Williams,BD. Kana,V. Mizrahi J. Bacteriol. 2011	mwilliams		20971904	Expression in M. smegmatis moaD mutant restores MoCo biosynthesis
Citation	Characterisation of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a nonsynonymous SNP in the Mycobacterium bovis BCG orthologue. P. Mendoza Lopez,P. Golby,E. Wooff,JN. Garcia,MC. Garcia Pelayo,K. Conlon,A. Gema Camacho,RG. Hewinson,J. Polaina,A. Surez Garca,SV. Gordon Microbiology (Reading, England) 2010	mwilliams		20378651	Rv3124 is a positive transcriptional regulator of moaA1-moaB1-moaC1-moaD1

Property

Value

Creator

Evidence

PMID

Comment

Interaction

PhysicalInteraction Rv3324c

priti.priety

NAS

Co-occurrence(Functional-linkage)
authors,PR. Marri,JP. Bannantine,GB. Golding Comparative genomics of metabolic pathways in Mycobacterium species: gene duplication, gene decay and lateral gene transfer. FEMS Microbiol. Rev. 2006

Interaction

PhysicalInteraction Rv3324A

priti.priety

NAS

Interaction

PhysicalInteraction Rv3324A

priti.priety

NAS

Co-occurrence(Functional-linkage)
authors,MS. Cortese,AB. Caplan,RL. Crawford Structural, functional, and evolutionary analysis of moeZ, a gene encoding an enzyme required for the synthesis of the Pseudomonas metabolite, pyridine-2,6-bis(thiocarboxylic acid). BMC Evol. Biol. 2002

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Geneneighbourhood
authors,W. Zhang,A. Urban,H. Mihara,S. Leimkhler,T. Kurihara,N. Esaki IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. J. Biol. Chem. 2010

Citation

Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Nature 2001

priyadarshinipriyanka2001

ISS

11713534

Geneneighbourhood

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Geneneighbourhood
authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. Nature 2001

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Geneneighbourhood
authors,MW. Lake,MM. Wuebbens,KV. Rajagopalan,H. Schindelin Mechanism of ubiquitin activation revealed by the structure of a bacterial MoeB-MoaD complex. Nature 2001

Citation

Revisiting the evolution of Mycobacterium bovis. authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr J. Bacteriol. 2005

priyadarshinipriyanka2001

ISS

16159772

Geneneighbourhood

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Geneneighbourhood
authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr Revisiting the evolution of Mycobacterium bovis. J. Bacteriol. 2005

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Geneneighbourhood
authors,S. Mostowy,J. Inwald,S. Gordon,C. Martin,R. Warren,K. Kremer,D. Cousins,MA. Behr Revisiting the evolution of Mycobacterium bovis. J. Bacteriol. 2005

Citation

IscS functions as a primary sulfur-donating enzyme by interacting specifically with MoeB and MoaD in the biosynthesis of molybdopterin in Escherichia coli. authors,W. Zhang,A. Urban,H. Mihara,S. Leimkhler,T. Kurihara,N. Esaki J. Biol. Chem. 2010

priyadarshinipriyanka2001

ISS

19946146

Geneneighbourhood

Interaction

Operon Rv3112

priyadarshinipriyanka2001

ISS

Citation

Insights from the complete genome sequence of Mycobacterium marinum on the evolution of Mycobacterium tuberculosis. authors,TP. Stinear,T. Seemann,PF. Harrison,GA. Jenkin,JK. Davies,PD. Johnson,Z. Abdellah,C. Arrowsmith,T. Chillingworth,C. Churcher,K. Clarke,A. Cronin,P. Davis,I. Goodhead,N. Holroyd,K. Jagels,A. Lord,S. Moule,K. Mungall,H. Norbertczak,MA. Quail,E. Rabbinowitsch,D. Walker,B. White,S. Whitehead,PL. Small,R. Brosch,L. Ramakrishnan,MA. Fischbach,J. Parkhill,ST. Cole Genome Res. 2008

mwilliams

18403782

Acquired by horizontal gene transfer

Citation

High content phenotypic cell-based visual screen identifies Mycobacterium tuberculosis acyltrehalose-containing glycolipids involved in phagosome remodeling. authors,P. Brodin,Y. Poquet,F. Levillain,I. Peguillet,G. Larrouy-Maumus,M. Gilleron,F. Ewann,T. Christophe,D. Fenistein,J. Jang,MS. Jang,SJ. Park,J. Rauzier,JP. Carralot,R. Shrimpton,A. Genovesio,JA. Gonzalo-Asensio,G. Puzo,C. Martin,R. Brosch,GR. Stewart,B. Gicquel,O. Neyrolles PLoS Pathog. 2010

mwilliams

20844580

Transposon mutant in M. tuberculosis W-Beijing strain was defective in arresting phagosome maturation

Citation

Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a fused molybdopterin synthase in Mycobacterium tuberculosis. authors,MJ. Williams,BD. Kana,V. Mizrahi J. Bacteriol. 2011

mwilliams

20971904

Deletion of moaA1-moaB1-moaC1-moaD1 in M. tuberculosis results in reduced MoCo biosynthesis

Citation

Functional analysis of molybdopterin biosynthesis in mycobacteria identifies a fused molybdopterin synthase in Mycobacterium tuberculosis. authors,MJ. Williams,BD. Kana,V. Mizrahi J. Bacteriol. 2011

mwilliams

20971904

Expression in M. smegmatis moaD mutant restores MoCo biosynthesis

Citation

Characterisation of the transcriptional regulator Rv3124 of Mycobacterium tuberculosis identifies it as a positive regulator of molybdopterin biosynthesis and defines the functional consequences of a nonsynonymous SNP in the Mycobacterium bovis BCG orthologue. P. Mendoza Lopez,P. Golby,E. Wooff,JN. Garcia,MC. Garcia Pelayo,K. Conlon,A. Gema Camacho,RG. Hewinson,J. Polaina,A. Surez Garca,SV. Gordon Microbiology (Reading, England) 2010

mwilliams

20378651

Rv3124 is a positive transcriptional regulator of moaA1-moaB1-moaC1-moaD1

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