TB Genome Annotation Portal

Rv2935 (ppsE)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: non-ribosomal peptide synthetase domain-containing protein
  • REFSEQ: phenolpthiocerol synthesis type-I polyketide synthase PPSE
  • PATRIC: Malonyl CoA-acyl carrier protein transacylase (EC 2.3.1.39)
  • TUBERCULIST: Phenolpthiocerol synthesis type-I polyketide synthase PpsE
  • NCBI: Phenolpthiocerol synthesis type-I polyketide synthase PpsE
  • updated information (H37Rv4):
    • gene name: ppsE
    • function:
    • reference:
• Type: Not Target
• Start: 3267737
• End: 3272203
• Operon:


• Trans-membrane region:
  
• Role: I.I - Polyketide and non-ribosomal peptide synthesis
• GO terms:
  • GO:0071770 - DIM/DIP cell wall layer assembly (Uniprot)
  • GO:0071766 - Actinobacterium-type cell wall biogenesis (Uniprot)
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0034081 - polyketide synthase complex (Uniprot)
  • GO:0031177 - phosphopantetheine binding (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0008610 - lipid biosynthetic process (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0006633 - fatty acid biosynthetic process (Uniprot)
  • GO:0006631 - fatty acid metabolic process (Uniprot)
  • GO:0006629 - lipid metabolic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0004315 - 3-oxoacyl-[acyl-carrier-protein] synthase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • 2 SMMALONYLCOA[c] + 3 MALCOA[c] + PHPAA_COA[c] -> 5 CO2[c] + 5 COA[c] + PHENOLPHTHIODIOLONE[A][c]
  • ATP[c] + 5 NADPH[c] + 2 SMMALONYLCOA[c] + 3 MALCOA[c] + DOCOSANOYL_COA[c] -> 5 NADP[c] + PPI[c] + 5 CO2[c] + AMP[c] + PHTHIODIO... <Preview truncated at 128 characters>
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4WKY	51%	Streptomyces albus	Streptomcyes albus JA3453 oxazolomycin ketosynthase domain OzmN KS2
  4OQJ	45%	Streptomyces albus	Streptomcyes albus JA3453 oxazolomycin ketosynthase domain OzmQ KS1
  6MHK	43%	Bacillus amyloliquefaciens	Crystal structure of ketosynthase nine from bacillaene polyketide synthase in Bacillus amyloliquefaciens
  4ZDN	42%	Streptomyces platensis subsp. rosaceus	Streptomyces platensis isomigrastatin ketosynthase domain MgsF KS4
  4TKT	42%	Streptomyces platensis subsp. rosaceus	Streptomyces platensis isomigrastatin ketosynthase domain MgsF KS6
  4QYR	42%	Streptomyces platensis subsp. rosaceus	Streptomyces platensis isomigrastatin ketosynthase domain MgsE KS3
  5ERF	41%	Bacillus subtilis (strain 168)	Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168
  5ENY	41%	Bacillus subtilis	Ketosynthase from module 6 connected to acyl carrier protein from module 5 (unobservable) of the bacillaene synthase from Bacillus subtilis 168
  5ELP	41%	Bacillus amyloliquefaciens	Ketosynthase from module 1 of the bacillaene synthase from Bacillus amyloliquefaciens FZB42
  5E6K	41%	Bacillus subtilis (strain 168)	Ketosynthase from module 6 of the bacillaene synthase from Bacillus subtilis 168 (C167S mutant, crystal form 2)

• Links to additional information on ppsE:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.607 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VSIPENAIAVVGMAGRFPGAKDVSAFWSNLRRGKESIVTLSEQELRDAGVSDKTLADPAYVRRAPLLDGIDEFDAGFFGFPPLAAQVLDPQHRLFLQCAW
HALEDAGADPARFDGSIGVYGTSSPSGYLLHNLLSHRDPNAVLAEGLNFDQFSLFLQNDKDFLATRISHAFNLRGPSIAVQTACSSSLVAVHLACLSLLS
GECDMALAGGSSLCIPHRVGYFTSPGSMVSAVGHCRPFDVRADGTVFGSGVGLVVLKPLAAAIDAGDRIHAVIRGSAINNDGSAKMGYAAPNPAAQADVI
AEAHAVSGIDSSTVSYVECHGTGTPLGDPIEIQGLRAAFEVSQTSRSAPCVLGSVKSNIGHLEVAAGIAGLIKTILCLKNKALPATLHYTSPNPELRLDQ
SPFVVQSKYGPWECDGVRRAGVSSFGVGGTNAHVVLEEAPAEASEVSAHAEPAGPQVILLSAQTAAALGESRTALAAALETQDGPRLSDVAYTLARRRKH
NVTMAAVVHDREHAATVLRAAEHDNVFVGEAAHDGEHGDRADAAPTSDRVVFLFPGQGAQHVGMAKGLYDTEPVFAQHFDTCAAGFRDETGIDLHAEVFD
GTATDLERIDRSQPALFTVEYALAKLVDTFGVRAGAYIGYSTGEYIAATLAGVFDLQTAIKTVSLRARLMHESPPGAMVAVALGPDDVTQYLPPEVELSA
VNDPGNCVVAGPKDQIRALRQRLTEAGIPVRRVRATHAFHTSAMDPMLGQFQEFLSRQQLRPPRTPLLSNLTGSWMSDQQVVDPASWTRQISSPIRFADE
LDVVLAAPSRILVEVGPGGSLTGSAMRHPKWSTTHRTVRLMRHPLQDVDDRDTFLRALGELWSAGVEVDWTPRRPAVPHLVSLPGYPFARQRHWVEPNHT
VWAQAPGANNGSPAGTADGSTAATVDAARNGESQTEVTLQRIWSQCLGVSSVDRNANFFDLGGDSLMAISIAMAAANEGLTITPQDLYEYPTLASLTAAV
DASFASSGLAKPPEAQANPAVPPNVTYFLDRGLRDTGRCRVPLILRLDPKIGLPDIRAVLTAVVNHHDALRLHLVGNDGIWEQHIAAPAEFTGLSNRSVP
NGVAAGSPEERAAVLGILAELLEDQTDPNAPLAAVHIAAAHGGPHYLCLAIHAMVTDDSSRQILATDIVTAFGQRLAGEEITLEPVSTGWREWSLRCAAL
ATHPAALDTRSYWIENSTKATLWLADALPNAHTAHPPRADELTKLSSTLSVEQTSELDDGRRRFRRSIQTILLAALGRTIAQTVGEGVVAVELEGEGRSV
LRPDVDLRRTVGWFTTYYPVPLACATGLGALAQLDAVHNTLKSVPHYGIGYGLLRYVYAPTGRVLGAQRTPDIHFRYAGVIPELPSGDAPVQFDSDMTLP
VREPIPGMGHAIELRVYRFGGSLHLDWWYDTRRIPAATAEALERTFPLALSALIQEAIAAEHTEHDDSEIVGEPEAGALVDLSSMDAG

Additional Information