TB Genome Annotation Portal

Rv2384 (mbtA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: (23-dihydroxybenzoyl)adenylate synthase
  • REFSEQ: bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase
  • PATRIC: Bifunctional salicyl-AMP ligase/salicyl-S-arcp synthetase MbtA
  • TUBERCULIST: Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase
  • NCBI: Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ArCP synthetase
  • updated information (H37Rv4):
    • gene name: mbtA
    • function:
    • reference:
• Type: Not Target
• Start: 2675936
• End: 2677633
• Operon:


• Trans-membrane region:
  
• Role: I.I - Polyketide and non-ribosomal peptide synthesis
• GO terms:
  • GO:0019540 - siderophore biosynthetic process from catechol (Uniprot)
  • GO:0016881 - acid-amino acid ligase activity (Uniprot)
  • GO:0016874 - ligase activity (Uniprot)
  • GO:0010106 - cellular response to iron ion starvation (Uniprot)
  • GO:0008668 - (2,3-dihydroxybenzoyl)adenylate synthase activity (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ATP[c] + SAL[c] + MBT_HOLO[c] -> PPI[c] + AMP[c] + MBTA_SAL[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5KEI	70%	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	Mycobacterium smegmatis MbtA apo structure
  5WM7	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with AMP
  5WM6	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with Benzoyl Adenylate
  5WM5	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with 5-Methylsalicyl Adenylate
  5WM4	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with 6-Methylsalicyl Adenylate
  5WM3	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with Salicyl Adenylate
  5WM2	54%	Streptomyces gandocaensis	Crystal Structure of CahJ in Complex with Salicylic Acid and AMP
  6E97	47%	Thermobifida fusca	Crystal structure of the aryl acid adenylating enzyme FscC from Fuscachelin NRPS in complex with DHB-adenylate
  6E8O	47%	Thermobifida fusca	Crystal structure of aryl acid adenylating enzyme FscC from Fuscachelin NRPS in complex with AMP
  1MDF	44%	Bacillus subtilis	CRYSTAL STRUCTURE OF DhbE IN ABSENCE OF SUBSTRATE

• Links to additional information on mbtA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.168 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MPPKAADGRRPSPDGGLGGFVPFPADRAASYRAAGYWSGRTLDTVLSDAARRWPDRLAVADAGDRPGHGGLSYAELDQRADRAAAALHGLGITPGDRVLL
QLPNGCQFAVALFALLRAGAIPVMCLPGHRAAELGHFAAVSAATGLVVADVASGFDYRPMARELVADHPTLRHVIVDGDPGPFVSWAQLCAQAGTGSPAP
PADPGSPALLLVSGGTTGMPKLIPRTHDDYVFNATASAALCRLSADDVYLVVLAAGHNFPLACPGLLGAMTVGATAVFAPDPSPEAAFAAIERHGVTVTA
LVPALAKLWAQSCEWEPVTPKSLRLLQVGGSKLEPEDARRVRTALTPGLQQVFGMAEGLLNFTRIGDPPEVVEHTQGRPLCPADELRIVNADGEPVGPGE
EGELLVRGPYTLNGYFAAERDNERCFDPDGFYRSGDLVRRRDDGNLVVTGRVKDVICRAGETIAASDLEEQLLSHPAIFSAAAVGLPDQYLGEKICAAVV
FAGAPITLAELNGYLDRRGVAAHTRPDQLVAMPALPTTPIGKIDKRAIVRQLGIATGPVTTQRCH

Additional Information