Rv1894c (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: hypothetical protein
- REFSEQ: hypothetical protein
- PATRIC: 2-nitropropane dioxygenase (EC 1.13.11.32)
- TUBERCULIST: Conserved hypothetical protein
- NCBI: hypothetical protein
- updated information (H37Rv4):
  - gene name: nmo1
  - function: nitronate monooxygenase
  - reference: Klinkenberg & Karakousis (2013) PMID: 23408846
Type: Not Target
Start: 2140739
End: 2141869
Operon:

Trans-membrane region:
Role: I.B.7 - Miscellaneous oxidoreductases and oxygenases
GO terms:
- GO:0055114 - oxidation-reduction process (Uniprot)
- GO:0018580 - nitronate monooxygenase activity (Uniprot)
- GO:0003824 - catalytic activity (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on Rv1894c:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 1.2 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MHTAICDELGIEFPIFAFTHCRDVVVAVSKAGGFGVLGAVGFTPEQLEIELNWIDEHIGDHPYGVDIVIPNKYEGMDSQLSADELAKTLRSMVPQEHLDF
ARKILADHGVPVEDADEDSLQLLGWTEATATPQVDAALKHPKMTMVANALGTPPADMIKHIHDSGRKVAALCGSPSQARKHADAGVDIIIAQGGEAGGHC
GEVGSIVLWPQVVKEVAPVPVLAAGGIGSGQQIAAALALGTQGAWTGSQWLMVEEAANTAVQQAAYVKATSRDTVRSRSFTGKPARMLRNDWTEAWEQPE
SPKPLGMPLQYMVSGMAVKATHKYPNETVDVAFNPVGQVVGQFTKVEKTATVIERWVQEYLEATARLDALNAAASV

(Nucleotide sequence available on KEGG)

Additional Information

nitronate monooxygenase (nmo1)
upregulated in hypoxia, required for virulence in guinea pigs
Klinkenberg & Karakousis (2013). JID. http://www.ncbi.nlm.nih.gov/pubmed/23408846

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 14 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 2 non-insertions in a row out of 14 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 14 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 2 non-insertions in a row out of 14 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 14 sites
Non-Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Non-Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.54
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv1033c (trcR) (Direct binding)
- Rv3574 (kstR) (Direct binding)
- Rv3597c (lsr2) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Rv0023 (-)
- Rv0818 (-)
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv3574 (kstR)
- Rv3849 (espR)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	Regulatory Rv3574	priyadarshinipriyanka2001	IEP		Co-expression (Functional linkage) SL. Kendall,P. Burgess,R. Balhana,M. Withers,A. Ten Bokum,JS. Lott,C. Gao,I. Uhia Castro,NG. Stoker Cholesterol utilisation in mycobacteria is controlled by two TetR-type transcriptional regulators; kstR and kstR2. Microbiology (Reading, England) 2010
Interaction	Regulatory Rv3574	priyadarshinipriyanka2001	IEP		Co-expression (Functional linkage) SL. Kendall, M. Withers et al. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Mol. Microbiol. 2007
Citation	A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. SL. Kendall, M. Withers et al. Mol. Microbiol. 2007	aparna.vchalam	IEP	17635188	Co-expression (Functional llnkage)
Interaction	RegulatedBy Rv3574	aparna.vchalam	IEP		Co-expression (Functional llnkage) SL. Kendall, M. Withers et al. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Mol. Microbiol. 2007
Citation	Metronidazole lacks activity against Mycobacterium tuberculosis in an in vivo hypoxic granuloma model of latency. authors,LG. Klinkenberg,LA. Sutherland,WR. Bishai,PC. Karakousis J. Infect. Dis. 2008	aparna.vchalam	IEP	18491971	Co-expression (Functional llnkage)
Interaction	RegulatedBy Rv3574	aparna.vchalam	IEP		Co-expression (Functional llnkage) authors,LG. Klinkenberg,LA. Sutherland,WR. Bishai,PC. Karakousis Metronidazole lacks activity against Mycobacterium tuberculosis in an in vivo hypoxic granuloma model of latency. J. Infect. Dis. 2008
Citation	A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. SL. Kendall, M. Withers et al. Mol. Microbiol. 2007	priyadarshinipriyanka2001	IEP	17635188	Co-expression (Functional llnkage)
Interaction	RegulatedBy Rv3574	priyadarshinipriyanka2001	IEP		Co-expression (Functional llnkage) SL. Kendall, M. Withers et al. A highly conserved transcriptional repressor controls a large regulon involved in lipid degradation in Mycobacterium smegmatis and Mycobacterium tuberculosis. Mol. Microbiol. 2007
Citation	Metronidazole lacks activity against Mycobacterium tuberculosis in an in vivo hypoxic granuloma model of latency. authors,LG. Klinkenberg,LA. Sutherland,WR. Bishai,PC. Karakousis J. Infect. Dis. 2008	priyadarshinipriyanka2001	IEP	18491971	Co-expression (Functional llnkage)
Interaction	RegulatedBy Rv3574	priyadarshinipriyanka2001	IEP		Co-expression (Functional llnkage) authors,LG. Klinkenberg,LA. Sutherland,WR. Bishai,PC. Karakousis Metronidazole lacks activity against Mycobacterium tuberculosis in an in vivo hypoxic granuloma model of latency. J. Infect. Dis. 2008
Interaction	RegulatedBy Rv0348	yamir.moreno	IEP		Microarrays. mRNA levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using microarray (or macroarray) experiments.. B. Abomoelak, EA. Hoye et al. mosR, a novel transcriptional regulator of hypoxia and virulence in Mycobacterium tuberculosis. J. Bacteriol. 2009
Interaction	RegulatedBy Rv3574	yamir.moreno	TAS		Literature previously reported link (from Balazsi et al. 2008). Traceable author statement to experimental support. G. Balzsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008

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