TB Genome Annotation Portal

Rv1318c (-)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: adenylate cyclase
  • REFSEQ: adenylate cyclase
  • PATRIC: Adenylate cyclase (EC 4.6.1.1)
  • TUBERCULIST: Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase)
  • NCBI: Possible adenylate cyclase (ATP pyrophosphate-lyase) (adenylyl cyclase)
  • updated information (H37Rv4):
    • gene name: -
    • function:
    • reference:
• Type: Not Target
• Start: 1479199
• End: 1480824
• Operon:


• Trans-membrane region:
  
• Role: IV.J - Cyclases
• GO terms:
  • GO:0035556 - intracellular signal transduction (Uniprot)
  • GO:0016849 - phosphorus-oxygen lyase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0009190 - cyclic nucleotide biosynthetic process (Uniprot)
  • GO:0007165 - signal transduction (Uniprot)
  • GO:0006171 - cAMP biosynthetic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0004871 - signal transducer activity (Uniprot)
  • GO:0004016 - adenylate cyclase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ATP[c] -> PPI[c] + cAMP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4P2X	35%	Mycobacterium tuberculosis	Swapped Dimer of Mycobacterial Adenylyl cyclase Rv1625c: Form 2
  4P2M	35%	Mycobacterium tuberculosis	Swapped Dimer of Mycobacterial Adenylyl cyclase Rv1625c: Form 1
  4P2F	35%	Mycobacterium tuberculosis	Monomeric form of a single mutant (F363R) of Mycobacterial Adenylyl cyclase Rv1625c

• Links to additional information on Rv1318c:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.413 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MSAKKSTAQRLGRVLETVTRQSGRLPETPAYGSWLLGRVSESQRRRRVRIQVMLTALVVTANLLGIGVALLLVTIAIPEPSIVRDTPRWLTFGVVPGYVL
LALALGSYALTRQTVQALRWAIEGRKPTREEERRTFLAPWRVAVGHLMFWGVGTALLTTLYGLINNAFIPRFLFAVSFCGVLVATATYLHTEFALRPFAA
QALEAGPPPRRLAPGILGRTMVVWLLGSGVPVVGIALMAMFEMVLLNLTRMQFATGVLIISMVTLVFGFILMWILAWLTATPVRVVRAALRRVERGELRT
NLVVFDGTELGELQRGFNAMVAGLRERERVRDLFGRHVGREVAAAAERERSKLGGEERHVAVVFIDIVGSTQLVTSRPPADVVKLLNKFFAIVVDEVDRH
HGLVNKFEGDASLTIFGAPNRLPCPEDKALAAARAIADRLVNEMPECQAGIGVAAGQVIAGNVGARERFEYTVIGEPVNEAARLCELAKSRPGKLLASAQ
AVDAASEEERARWSLGRHVKLRGHDQPVRLAKPVGLTKPRR

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	2.61 (0.62)	1.56 (0.87)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link