Rv0941c (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: anti-anti-sigma factor
- REFSEQ: hypothetical protein
- PATRIC: FIG00823384: hypothetical protein
- TUBERCULIST: Conserved hypothetical protein
- NCBI: hypothetical protein
- updated information (H37Rv4):
  - gene name: -
  - function:
  - reference:
Type: Not Target
Start: 1051544
End: 1052317
Operon:

Trans-membrane region:
Role: VI - Unknowns
GO terms:
- No terms assigned.
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on Rv0941c:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 1.363 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

VVAVSTAAKSPTALAIAVRTQDSVVILTADGALDSSSSALLRDSLTRATLEQPSAVIVNVTELQVAEESAWSVFISARWQADFRADVPVLLVCGHRAGRA
AVTRTGVARFMPVYPTEKAASKAIGRLARRNFKRSDAQLPANLNSLRESRQLVREWLTQWSRPGLIPVALVVVNVFVENVLKHTGSDPVMRIESDGPTAT
IAVSDGSSAPAVRLASPPKGIDVSGLAIVAALSRAWGSSPTSSGKTVWAIIGPENQL

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 9 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 9 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 9 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 9 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 9 sites
Non-Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Non-Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 1.35
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0967 (csoR) (Direct binding)
- Rv3286c (sigF) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Rv3286c (sigF)
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv2703 (sigA)
- Rv2887 (-)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Structural Analysis AE. Greenstein, JA. MacGurn et al. M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. PLoS Pathog. 2007
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Spectrophotometric Assay authors,H. Kovacs,D. Comfort,M. Lord,ID. Campbell,MD. Yudkin Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 1998
Citation	The STAS domain - a link between anion transporters and antisigma-factor antagonists. authors,L. Aravind,EV. Koonin Curr. Biol. 2000	yashabhasin	ISA	10662676	Spectrophotometric Assay
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Spectrophotometric Assay authors,L. Aravind,EV. Koonin The STAS domain - a link between anion transporters and antisigma-factor antagonists. Curr. Biol. 2000
Citation	M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. AE. Greenstein, JA. MacGurn et al. PLoS Pathog. 2007	yashabhasin	ISA	17411339	Spectrophotometric Assay
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Spectrophotometric Assay AE. Greenstein, JA. MacGurn et al. M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. PLoS Pathog. 2007
Citation	Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis. authors,H. Kovacs,D. Comfort,M. Lord,ID. Campbell,MD. Yudkin Proc. Natl. Acad. Sci. U.S.A. 1998	yashabhasin	ISA	9560229	Structural Analysis
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Structural Analysis authors,H. Kovacs,D. Comfort,M. Lord,ID. Campbell,MD. Yudkin Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 1998
Citation	The STAS domain - a link between anion transporters and antisigma-factor antagonists. authors,L. Aravind,EV. Koonin Curr. Biol. 2000	yashabhasin	ISA	10662676	Structural Analysis
Interaction	PhysicalInteraction Rv3286c	yashabhasin	ISA		Structural Analysis authors,L. Aravind,EV. Koonin The STAS domain - a link between anion transporters and antisigma-factor antagonists. Curr. Biol. 2000
Citation	M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. AE. Greenstein, JA. MacGurn et al. PLoS Pathog. 2007	yashabhasin	ISA	17411339	Structural Analysis
Citation	Solution structure of SpoIIAA, a phosphorylatable component of the system that regulates transcription factor sigmaF of Bacillus subtilis. authors,H. Kovacs,D. Comfort,M. Lord,ID. Campbell,MD. Yudkin Proc. Natl. Acad. Sci. U.S.A. 1998	yashabhasin	ISA	9560229	Spectrophotometric Assay

TB Genome Annotation Portal