Rv0834c (PE_PGRS14)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: PE-PGRS family protein
- REFSEQ: PE-PGRS family protein
- PATRIC: PE_PGRS
- TUBERCULIST: PE-PGRS family protein PE_PGRS14
- NCBI: PE-PGRS family protein PE_PGRS14
- updated information (H37Rv4):
  - gene name: PE_PGRS14
  - function:
  - reference:
Type: Not Target
Start: 927837
End: 930485
Operon:

Trans-membrane region:
Role: IV.C.1.b - PE_PGRS subfamily
GO terms:
- No terms assigned.
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020):

PDB aa ident species PDB title

5XFS 45% Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Crystal structure of PE8-PPE15 in complex with EspG5 from M. tuberculosis
Links to additional information on PE_PGRS14:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.722 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

PDB	aa ident	species	PDB title
5XFS	45%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal structure of PE8-PPE15 in complex with EspG5 from M. tuberculosis

Amino Acid Sequence

MSFVIAAPDLVAMATEDLAGIGASLTAANAAAAVPTSGLLAAAGDEVSAAIAALFSSHGQQYQAMSAQAAAFHARFVQALAGAMGAYAAAEAANASPLQT
LEQGLLGAINAPAAALSGRPFIGNGTNGAPGTGEAGGPGGWLLGNGGNGGSGAPGQTGGAGGAAGLLGHGGTGGAGGTGASGGKGGTGGWLWGSGGAGGA
GGSGGGSGGAGGNALMFGIGGNGGAGGAASGVGNGGVGGAGGAGGALVAIGGAGGAGGAATTGTGGAGGAGSNALGLFLGLGGSGGQGGDSAMGSGGAGG
AGGSGGAASPFGIDIGIGGAGGHGGAGTNGGAGGAGGAGGSSGTVFALDLSWGGAGGNGGAATTGTGGAGGTGGFAVAPDFIGFGAAYGGAGGLGGAATG
AGGTGGTGGVGAGGFAALGVGVGGAGGAGGAATETGGIGGAGGLGVGLLGGAGGAGGPGGAASAGSGGHGGTGGDALGLIGAGIGGVGGVGGAATDTGGN
GGAGGSGTGLLGGVGGAGGHGGGASVGTGGSGGAGGDGFGFVGAGGNGGNAGTGVGVNGANGGNGGSATGALAAVGGAGAAGGDATSGTGGFGGAGGSAR
GLIFALGGAGAAGGDASTGVGGPGGPGGTGTASSPFGIAIAIGGAGAQGGAGTSGATGGAGGDGVFEGIAVLGLGFGGAAGAGGAATGDGATGGAGGFGG
AGAGIANFLGFSVLHGGAGGAGGTATGTGGNGGAGGGGGLSSPVILGIGIGGAGGDGGGALGVLGGMGGDGGDGGEAVAVGIAVGGAGGAGGAAPTGNGG
AGGNGGDALGLVGVGGNGGNAGTGFGANTGGNGGDTTIVVNGMLAPSTLGYGGNGGNGVNGGAGGTGGKAGVFGAPGQNGLP

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Uncertain	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.037000; 4 non-insertions in a row out of 27 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.005750; 3 non-insertions in a row out of 27 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 27 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000200; 2 non-insertions in a row out of 28 sites
Uncertain	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.169250; 5 non-insertions in a row out of 28 sites
No-Data	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Too-Short	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: -1
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

RNA processing and modification

Energy production and conversion

Chromatin structure and dynamics

Amino acid transport and metabolism

Cell cycle control, cell division, chromosome partitioning

Carbohydrate transport and metabolism

Nucleotide transport and metabolism

Lipid transport and metabolism

Coenzyme transport and metabolism

Transcription

Translation, ribosomal structure and biogenesis

Cell wall/membrane/envelope biogenesis

Replication, recombination and repair

Posttranslational modification, protein turnover, chaperones

Cell motility

Secondary metabolites biosynthesis, transport and catabolism

Inorganic ion transport and metabolism

Function unknown

General function prediction only

Intracellular trafficking, secretion, and vesicular transport

Signal transduction mechanisms

Extracellular structures

Defense mechanisms

Nuclear structure

Cytoskeleton

BioCyc Co-regulated genes based on gene expression profiling (Systems Biology, Inferelator Network)

Differentially expressed as result of RNASeq in glycerol environment (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

Conditionally essential as result of TNSeq (Only top 20 genes shown sorted by log fold change with p_adj 0.05).

BioCyc Transcription factor binding based on ChIP-Seq (Systems Biology)

Binds To:
- No bindings to other targets were found.
Bound By:
- No bindings from other targets were found.

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0047c (-) (Direct binding)
- Rv3830c (-) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Not downregulated by other genes.

Property	Value	Creator	Evidence	PMID	Comment
Citation	Usefulness of acr expression for monitoring latent Mycobacterium tuberculosis bacilli in 'in vitro' and 'in vivo' experimental models. S. Gordillo, E. Guirado et al. Scand. J. Immunol. 2006	jlew		16784488	Not detected in the experiments with EP bacilli, can be detected with SP ones from days 1 to 4 when the pH drops from 5.5 to 4.5.. Exponential and stationary phase bacilli were exposed to decreasing pH levels (from 6.5 to 4.5) in an unstirred culture, and mRNA levels for 16S rRNA, sigma factors sigA,B,E,F,G,H and M, Rv0834c, icl, nirA, narG, fpbB, acr, rpoA, recA and cysH were quantified.
Citation	Variable expression patterns of Mycobacterium tuberculosis PE_PGRS genes: evidence that PE_PGRS16 and PE_PGRS26 are inversely regulated in vivo. V. Dheenadhayalan, G. Delogu et al. J. Bacteriol. 2006	jlew		16672626	Constitutively expressed in all in vitro conditions examined. Evaluation of expression of 16 PE_PGRS genes present in Mycobacterium tuberculosis under various growth conditions

TB Genome Annotation Portal