TB Genome Annotation Portal

Rv0694 (lldD1)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: heme/flavin dehydrogenase mycofactocin system protein
  • REFSEQ: L-lactate dehydrogenase (cytochrome) LldD1
  • PATRIC: Mycofactocin system heme/flavin dehydrogenase
  • TUBERCULIST: Possible L-lactate dehydrogenase (cytochrome) LldD1
  • NCBI: Possible L-lactate dehydrogenase (cytochrome) LldD1
  • updated information (H37Rv4):
    • gene name: mftD
    • function: aka MftD - flavin/heme dehydrogenase; mycofactocin synthesis; (not relevant to LDH activity)
    • reference: Krishnamoorthy (2021) PMID: 34311585; Billig (2017) PMID: 28744015
• Type: Conditional
• Start: 793335
• End: 794525
• Operon:


• Trans-membrane region:
  
• Role: I.B.6.a - aerobic
• GO terms:
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0010181 - FMN binding (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • NAD[c] + LLAC[c] <=> NADH[c] + PYR[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6GMC	39%	Homo sapiens	1.2 A resolution structure of human hydroxyacid oxidase 1 bound with FMN and 4-carboxy-5-[(4-chlorophenyl)sulfanyl]-1,2,3-thiadiazole
  6GMB	39%	Homo sapiens	Structure of human hydroxyacid oxidase 1 bound with FMN and glycolate
  5QIH	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z2697514548
  5QIG	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z1407672867
  5QIF	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z31792168
  5QIE	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z2856434894
  5QID	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z1787627869
  5QIC	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with Z30620520
  5QIB	39%	Homo sapiens	PanDDA analysis group deposition of models with modelled events (e.g. bound ligands) -- Crystal Structure of HAO1 in complex with FMOPL000388a
  2W0U	39%	HOMO SAPIENS	CRYSTAL STRUCTURE OF HUMAN GLYCOLATE OXIDASE IN COMPLEX WITH THE INHIBITOR 5-[(4-CHLOROPHENYL)SULFANYL]- 1,2,3-THIADIAZOLE-4-CARBOXYLATE.

• Links to additional information on lldD1:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.23 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VAEAWFETVAIAQQRAKRRLPKSVYSSLIAASEKGITVADNVAAFSELGFAPHVIGATDKRDLSTTVMGQEVSLPVIISPTGVQAVDPGGEVAVARAAAA
RGTVMGLSSFASKPIEEVIAANPKTFFQVYWQGGRDALAERVERARQAGAVGLVVTTDWTFSHGRDWGSPKIPEEMNLKTILRLSPEAITRPRWLWKFAK
TLRPPDLRVPNQGRRGEPGPPFFAAYGEWMATPPPTWEDIGWLRELWGGPFMLKGVMRVDDAKRAVDAGVSAISVSNHGGNNLDGTPASIRALPAVSAAV
GDQVEVLLDGGIRRGSDVVKAVALGARAVMIGRAYLWGLAANGQAGVENVLDILRGGIDSALMGLGHASVHDLSPADILVPTGFIRDLGVPSRRDV

Additional Information

MftD - flavin/heme dehydrogenase; mycofactocin synthesis cluster
Krishnamoorthy G, Kaiser P, Lozza L, Hahnke K, Mollenkopf HJ, Kaufmann SHE. Mycofactocin is associated with ethanol metabolism in mycobacteria. mBio 10, (2019).
https://mbio.asm.org/content/10/3/e00190-19/article-info