TB Genome Annotation Portal

Rv0649 (fabD2)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: [acyl-carrier-protein] S-malonyltransferase
  • REFSEQ: malonyl CoA-acyl carrier protein transacylase
  • PATRIC: POSSIBLE MALONYL COA-ACYL CARRIER PROTEIN TRANSACYLASE FABD2 (MCT) (EC 2.3.1.39)
  • TUBERCULIST: Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT)
  • NCBI: Possible malonyl CoA-acyl carrier protein transacylase FabD2 (MCT)
  • updated information (H37Rv4):
    • gene name: fabD2
    • function:
    • reference:
• Type: Not Target
• Start: 746363
• End: 747037
• Operon:


• Trans-membrane region:
  
• Role: I.H.1 - Synthesis of fatty and mycolic acids
• GO terms:
  • GO:0042759 - long-chain fatty acid biosynthetic process (Uniprot)
  • GO:0004314 - [acyl-carrier-protein] S-malonyltransferase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ACCOA[c] + ACP[c] <=> COA[c] + ACACP[c]
  • COA[c] + HEXANOYL_ACP[c] <=> ACP[c] + HEXANOYL_COA[c]
  • COA[c] + TETRADECANOYL_ACP[c] <=> ACP[c] + TETRADECANOYL_COA[c]
  • COA[c] + HEXADECANOYL_ACP[c] <=> ACP[c] + HEXADECANOYL_COA[c]
  • COA[c] + 9_HEXADECENOYL_ACP[c] <=> ACP[c] + 9_HEXADECENOYL_COA[c]
  • COA[c] + OCTADECANOYL_ACP[c] <=> ACP[c] + OCTADECANOYL_COA[c]
  • COA[c] + 9_OCTADECENOYL_ACP[c] <=> ACP[c] + 9_OCTADECENOYL_COA[c]
  • COA[c] + EICOSANOYL_ACP[c] <=> ACP[c] + EICOSANOYL_COA[c]
  • COA[c] + DOCOSANOYL_ACP[c] <=> ACP[c] + DOCOSANOYL_COA[c]
  • COA[c] + TETRACOSANOYL_ACP[c] <=> ACP[c] + TETRACOSANOYL_COA[c]
  • COA[c] + HEXACOSANOYL_ACP[c] <=> ACP[c] + HEXACOSANOYL_COA[c]
  • COA[c] + OCTACOSANOYL_ACP[c] <=> ACP[c] + OCTACOSANOYL_COA[c]
  • COA[c] + NONADECANOYL_ACP[c] <=> ACP[c] + NONADECANOYL_COA[c]
  • COA[c] + HEPTADECANOYL_ACP[c] <=> ACP[c] + HEPTADECANOYL_COA[c]
  • COA[c] + PENTADECANOYL_ACP[c] <=> ACP[c] + PENTADECANOYL_COA[c]
  • PROPIONYLCOA[c] + ACP[c] -> COA[c] + PROPIONYLACP[c]
  • ACP[c] + MALCOA[c] -> COA[c] + MALACP[c]
  • SMMALONYLCOA[c] + ACP[c] -> COA[c] + RMMALONYLACP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
• Links to additional information on fabD2:
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.616 (from Jeremy Rock's lab)

Amino Acid Sequence

MSGRSRLPGSSSRRDAARIVAERVVATVAGVAVAVDEVDAAEARLRDGPRAAALPASGTSEGRQLRRWLTQLIVTERVVAAEAAARGLTAAGAPAEADLL
PDATARLEIGSVAAAVLADPLARALFAAVTARVAVTDDAVADYHARNPLRFAAPCPGQHGWRAPAAAAPPLDQVRRAITEHLLGAARRRAFRVWLDARRN
ALVVLAPGYEHPGDPRQPDNTRRH

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	0.9 (0.11)	1.64 (0.72)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link