TB Genome Annotation Portal

Rv3913 (trxB2)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: thioredoxin reductase
  • REFSEQ: thioredoxin reductase TRXB2
  • PATRIC: Thioredoxin reductase (EC 1.8.1.9)
  • TUBERCULIST: Probable thioredoxin reductase TrxB2 (TRXR) (TR)
  • NCBI: Probable thioredoxin reductase TrxB2 (TRXR) (TR)
  • updated information (H37Rv4):
    • gene name: trxB2
    • function:
    • reference: Lin (2016) PMID: 27249779
• Type: Not Target
• Start: 4401728
• End: 4402735
• Operon:


• Trans-membrane region:
  
• Role: I.G.10 - Thioredoxin, glutaredoxin and mycothiol
• GO terms:
  • GO:0070402 - NADPH binding (Uniprot)
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0050660 - flavin adenine dinucleotide binding (Uniprot)
  • GO:0045454 - cell redox homeostasis (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0019430 - removal of superoxide radicals (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0015035 - protein disulfide oxidoreductase activity (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0004791 - thioredoxin-disulfide reductase activity (Uniprot)
  • GO:0001666 - response to hypoxia (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • NADPH[c] + OTHIO[c] -> NAD[c] + RTHIO[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 2a87
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2A87	100%	Mycobacterium tuberculosis	Crystal Structure of M. tuberculosis Thioredoxin reductase
  5UTH	76%	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	Crystal structure of thioredoxin reductase from Mycobacterium smegmatis in complex with FAD
  1VDC	51%	Arabidopsis thaliana	STRUCTURE OF NADPH DEPENDENT THIOREDOXIN REDUCTASE
  1TDE	49%	Escherichia coli	CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFINED AT 2 ANGSTROM RESOLUTION: IMPLICATIONS FOR A LARGE CONFORMATIONAL CHANGE DURING CATALYSIS
  1CL0	49%	Escherichia coli	CRYSTAL STRUCTURE OF REDUCED THIOREDOXIN REDUCTASE FROM ESCHERICHIA COLI.
  2WHD	49%	Hordeum vulgare	Barley NADPH-dependent thioredoxin reductase 2
  1TRB	48%	Escherichia coli	CONVERGENT EVOLUTION OF SIMILAR FUNCTION IN TWO STRUCTURALLY DIVERGENT ENZYMES
  1TDF	48%	Escherichia coli	CRYSTAL STRUCTURE OF ESCHERICHIA COLI THIOREDOXIN REDUCTASE REFINED AT 2 ANGSTROM RESOLUTION: IMPLICATIONS FOR A LARGE CONFORMATIONAL CHANGE DURING CATALYSIS
  1F6M	48%	Escherichia coli	CRYSTAL STRUCTURE OF A COMPLEX BETWEEN THIOREDOXIN REDUCTASE, THIOREDOXIN, AND THE NADP+ ANALOG, AADP+
  5W4C	47%	Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487)	Crystal structure of thioredoxin reductase from Cryptococcus neoformans in complex with FAD (FO conformation)

• Links to additional information on trxB2:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -7.059 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MTAPPVHDRAHHPVRDVIVIGSGPAGYTAALYAARAQLAPLVFEGTSFGGALMTTTDVENYPGFRNGITGPELMDEMREQALRFGADLRMEDVESVSLHG
PLKSVVTADGQTHRARAVILAMGAAARYLQVPGEQELLGRGVSSCATCDGFFFRDQDIAVIGGGDSAMEEATFLTRFARSVTLVHRRDEFRASKIMLDRA
RNNDKIRFLTNHTVVAVDGDTTVTGLRVRDTNTGAETTLPVTGVFVAIGHEPRSGLVREAIDVDPDGYVLVQGRTTSTSLPGVFAAGDLVDRTYRQAVTA
AGSGCAAAIDAERWLAEHAATGEADSTDALIGAQR

Additional Information