Rv3875 (esxA)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: early secretory antigenic target
- REFSEQ: 6 kDa early secretory antigenic target ESXA (ESAT-6)
- PATRIC: 6 kDa early secretory antigenic target ESAT-6 (EsxA)
- TUBERCULIST: 6 kDa early secretory antigenic target EsxA (ESAT-6)
- NCBI: 6 kDa early secretory antigenic target EsxA (ESAT-6)
- updated information (H37Rv4):
  - gene name: esxA
  - function:
  - reference:
Type: Not Target
Start: 4352609
End: 4352896
Operon:

Trans-membrane region:
Role: II.C.2 - Surface polysaccharides, lipopolysaccharides, proteins and antigens
GO terms:
- GO:0052083 - negative regulation by symbiont of host cell-mediated immune response (Uniprot)
- GO:0052027 - modulation by symbiont of host signal transduction pathway (Uniprot)
- GO:0046812 - host cell surface binding (Uniprot)
- GO:0044764 - multi-organism cellular process (Uniprot)
- GO:0044315 - protein secretion by the type VII secretion system (Uniprot)
- GO:0044228 - host cell surface (Uniprot)
- GO:0044165 - host cell endoplasmic reticulum (Uniprot)
- GO:0042803 - protein homodimerization activity (Uniprot)
- GO:0033644 - host cell membrane (Uniprot)
- GO:0030430 - host cell cytoplasm (Uniprot)
- GO:0020002 - host cell plasma membrane (Uniprot)
- GO:0016021 - integral component of membrane (Uniprot)
- GO:0016020 - membrane (Uniprot)
- GO:0009405 - pathogenesis (Uniprot)
- GO:0005886 - plasma membrane (Uniprot)
- GO:0005737 - cytoplasm (Uniprot)
- GO:0005618 - cell wall (Uniprot)
- GO:0005576 - extracellular region (Uniprot)
- GO:0005515 - protein binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 3fav, 1wa8
Search for Homologs in PDB

Top 10 Homologs in PDB (as of Nov 2020):

PDB	aa ident	species	PDB title
3FAV	100%	Mycobacterium tuberculosis	Structure of the CFP10-ESAT6 complex from Mycobacterium tuberculosis
1WA8	100%	MYCOBACTERIUM TUBERCULOSIS	Solution Structure of the CFP-10.ESAT-6 Complex. Major Virulence Determinants of Pathogenic Mycobacteria

Links to additional information on esxA:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.723 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.035350; 2 non-insertions in a row out of 4 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.035300; 2 non-insertions in a row out of 4 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.036400; 2 non-insertions in a row out of 4 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.002150; 4 non-insertions in a row out of 5 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.002100; 4 non-insertions in a row out of 5 sites
No-Data	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Too-Short	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: -1
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv2034 (-)

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0081 (-) (Direct binding)
- Rv1985c (-) (Direct binding)
- Rv3597c (lsr2) (Direct binding)
- Rv0678 (-) (Upstream of operon)
- Rv1353c (-) (Upstream of operon)
- Rv2011c (-) (Upstream of operon)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv2034 (-)

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv3875 (esxA)

Property	Value	Creator	Evidence	PMID	Comment
Citation	Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. P. Brodin, L. Majlessi et al. Infect. Immun. 2006	shahanup86	IMP	16368961	Structural Analysis
Interaction	Regulatory Rv3872	shahanup86	IMP		Structural Analysis P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006
Citation	Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. A. Luthra, A. Mahmood et al. J. Biol. Chem. 2008	shahanup86	IMP	18974091	Structural Analysis
Interaction	Regulatory Rv3872	shahanup86	IMP		Structural Analysis A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008
Citation	Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. LM. Okkels & P. Andersen J. Bacteriol. 2004	shahanup86	IPI	15060053	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3874	shahanup86	IPI		Affinity purification (Physical interaction) LM. Okkels & P. Andersen Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. J. Bacteriol. 2004
Citation	EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. ML. Burts, WA. Williams et al. Proc. Natl. Acad. Sci. U.S.A. 2005	shahanup86	IPI	15657139	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3874	shahanup86	IPI		Affinity purification (Physical interaction) ML. Burts, WA. Williams et al. EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc. Natl. Acad. Sci. U.S.A. 2005
Citation	C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. PA. Champion, SA. Stanley et al. Science 2006	shahanup86	IMP	16973880	Spectrophotometric
Interaction	Regulatory Rv3872	shahanup86	IMP		Spectrophotometric PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006
Citation	Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. P. Brodin, L. Majlessi et al. Infect. Immun. 2006	shahanup86	IMP	16368961	Spectrophotometric
Interaction	Regulatory Rv3872	shahanup86	IMP		Spectrophotometric P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006
Citation	Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. A. Luthra, A. Mahmood et al. J. Biol. Chem. 2008	shahanup86	IMP	18974091	Spectrophotometric
Interaction	Regulatory Rv3872	shahanup86	IMP		Spectrophotometric A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008
Citation	Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr J. Biol. Chem. 2002	shahanup86	IMP	11940590	Structural Analysis
Interaction	Regulatory Rv3872	shahanup86	IMP		Structural Analysis authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. J. Biol. Chem. 2002
Citation	C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. PA. Champion, SA. Stanley et al. Science 2006	shahanup86	IMP	16973880	Structural Analysis
Interaction	Regulatory Rv3872	shahanup86	IMP		Structural Analysis PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006
Interaction	PhysicalInteraction Rv3874	shahanup86	IPI		Affinity purification (Physical interaction) LM. Okkels & P. Andersen Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. J. Bacteriol. 2004
Interaction	PhysicalInteraction Rv3874	shahanup86	IPI		Affinity purification (Physical interaction) ML. Burts, WA. Williams et al. EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc. Natl. Acad. Sci. U.S.A. 2005
Citation	Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr J. Biol. Chem. 2002	shahanup86	IMP	11940590	Spectrophotometric
Interaction	Regulatory Rv3872	shahanup86	IMP		Spectrophotometric authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. J. Biol. Chem. 2002
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006
Interaction	PhysicalInteraction Rv3872	shahanup86	IMP		Co-expression (Functional linkage) PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006
Interaction	RegulatedBy Rv3414c	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv2710	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv2021c	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv1221	yamir.moreno	IEP		Microarrays. mRNA levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using microarray (or macroarray) experiments.. R. Manganelli, MI. Voskuil et al. The Mycobacterium tuberculosis ECF sigma factor sigmaE: role in global gene expression and survival in macrophages. Mol. Microbiol. 2001
Citation	Over-producing soluble protein complex and validating protein-protein interaction through a new bacterial co-expression system. J. Zeng, L. Zhang et al. Protein Expr. Purif. 2010	jlew		19747546	Rv3874 and Rv3875 interact. Using an in vivo pull-down assay, for the first time we have confirmed the presence of three pairs of PE/PPE-related novel protein interactions in this pathogen.
Symbol	ESAT-6	jlew			Forms homodimer. Forms heterodimer with CFP-10.. Yeast two-hybrid to examine the interactions between ESX-1 proteins J. Teutschbein, G. Schumann et al. A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis. Microbiol. Res. 2007
Citation	A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis. J. Teutschbein, G. Schumann et al. Microbiol. Res. 2007	jlew		17433643	Forms homodimer. Forms heterodimer with CFP-10.. Yeast two-hybrid to examine the interactions between ESX-1 proteins

Property

Value

Creator

Evidence

PMID

Comment

Citation

Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. P. Brodin, L. Majlessi et al. Infect. Immun. 2006

shahanup86

IMP

16368961

Structural Analysis

Interaction

Regulatory Rv3872

shahanup86

IMP

Structural Analysis
P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006

Citation

Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. A. Luthra, A. Mahmood et al. J. Biol. Chem. 2008

shahanup86

IMP

18974091

Structural Analysis

Interaction

Regulatory Rv3872

shahanup86

IMP

Structural Analysis
A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008

Citation

Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. LM. Okkels & P. Andersen J. Bacteriol. 2004

shahanup86

IPI

15060053

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3874

shahanup86

IPI

Affinity purification (Physical interaction)
LM. Okkels & P. Andersen Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. J. Bacteriol. 2004

Citation

EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. ML. Burts, WA. Williams et al. Proc. Natl. Acad. Sci. U.S.A. 2005

shahanup86

IPI

15657139

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3874

shahanup86

IPI

Affinity purification (Physical interaction)
ML. Burts, WA. Williams et al. EsxA and EsxB are secreted by an ESAT-6-like system that is required for the pathogenesis of Staphylococcus aureus infections. Proc. Natl. Acad. Sci. U.S.A. 2005

Citation

C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. PA. Champion, SA. Stanley et al. Science 2006

shahanup86

IMP

16973880

Spectrophotometric

Interaction

Regulatory Rv3872

shahanup86

IMP

Spectrophotometric
PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006

Citation

Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. P. Brodin, L. Majlessi et al. Infect. Immun. 2006

shahanup86

IMP

16368961

Spectrophotometric

Interaction

Regulatory Rv3872

shahanup86

IMP

Spectrophotometric
P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006

Citation

Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. A. Luthra, A. Mahmood et al. J. Biol. Chem. 2008

shahanup86

IMP

18974091

Spectrophotometric

Interaction

Regulatory Rv3872

shahanup86

IMP

Spectrophotometric
A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008

Citation

Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr J. Biol. Chem. 2002

shahanup86

IMP

11940590

Structural Analysis

Interaction

Regulatory Rv3872

shahanup86

IMP

Structural Analysis
authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. J. Biol. Chem. 2002

Citation

C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. PA. Champion, SA. Stanley et al. Science 2006

shahanup86

IMP

16973880

Structural Analysis

Interaction

Regulatory Rv3872

shahanup86

IMP

Structural Analysis
PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006

Interaction

PhysicalInteraction Rv3874

shahanup86

IPI

Affinity purification (Physical interaction)
LM. Okkels & P. Andersen Protein-protein interactions of proteins from the ESAT-6 family of Mycobacterium tuberculosis. J. Bacteriol. 2004

Interaction

PhysicalInteraction Rv3874

shahanup86

IPI

Citation

shahanup86

IMP

11940590

Spectrophotometric

Interaction

Regulatory Rv3872

shahanup86

IMP

Spectrophotometric
authors,PS. Renshaw,P. Panagiotidou,A. Whelan,SV. Gordon,RG. Hewinson,RA. Williamson,MD. Carr Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6*CFP-10 complex. Implications for pathogenesis and virulence. J. Biol. Chem. 2002

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Co-expression (Functional linkage)
A. Luthra, A. Mahmood et al. Characterization of Rv3868, an Essential Hypothetical Protein of the ESX-1 Secretion System in Mycobacterium tuberculosis. J. Biol. Chem. 2008

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Co-expression (Functional linkage)
P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Co-expression (Functional linkage)
P. Brodin, L. Majlessi et al. Dissection of ESAT-6 system 1 of Mycobacterium tuberculosis and impact on immunogenicity and virulence. Infect. Immun. 2006

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Co-expression (Functional linkage)
PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006

Interaction

PhysicalInteraction Rv3872

shahanup86

IMP

Co-expression (Functional linkage)
PA. Champion, SA. Stanley et al. C-terminal signal sequence promotes virulence factor secretion in Mycobacterium tuberculosis. Science 2006

Interaction

RegulatedBy Rv3414c

yamir.moreno

IDA

One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

RegulatedBy Rv2710

yamir.moreno

IDA

Interaction

RegulatedBy Rv2021c

yamir.moreno

IDA

Interaction

RegulatedBy Rv1221

yamir.moreno

IEP

Microarrays. mRNA levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using microarray (or macroarray) experiments..
R. Manganelli, MI. Voskuil et al. The Mycobacterium tuberculosis ECF sigma factor sigmaE: role in global gene expression and survival in macrophages. Mol. Microbiol. 2001

Citation

Over-producing soluble protein complex and validating protein-protein interaction through a new bacterial co-expression system. J. Zeng, L. Zhang et al. Protein Expr. Purif. 2010

jlew

19747546

Rv3874 and Rv3875 interact. Using an in vivo pull-down assay, for the first time we have confirmed the presence of three pairs of PE/PPE-related novel protein interactions in this pathogen.

Symbol

ESAT-6

jlew

Forms homodimer. Forms heterodimer with CFP-10.. Yeast two-hybrid to examine the interactions between ESX-1 proteins
J. Teutschbein, G. Schumann et al. A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis. Microbiol. Res. 2007

Citation

A protein linkage map of the ESAT-6 secretion system 1 (ESX-1) of Mycobacterium tuberculosis. J. Teutschbein, G. Schumann et al. Microbiol. Res. 2007

jlew

17433643

Forms homodimer. Forms heterodimer with CFP-10.. Yeast two-hybrid to examine the interactions between ESX-1 proteins

Comments