Rv3792 (aftA)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: arabinofuranosyltransferase AftA
- REFSEQ: transmembrane protein
- PATRIC: putative membrane protein
- TUBERCULIST: Arabinofuranosyltransferase AftA
- NCBI: Arabinofuranosyltransferase AftA
- updated information (H37Rv4):
  - gene name: aftA
  - function:
  - reference:
Type: Essential
Start: 4237932
End: 4239863
Operon:

Trans-membrane region:
Role: II.C.5 - Other membrane proteins
GO terms:
- GO:0071769 - mycolate cell wall layer assembly (Uniprot)
- GO:0071555 - cell wall organization (Uniprot)
- GO:0045227 - capsule polysaccharide biosynthetic process (Uniprot)
- GO:0044038 - cell wall macromolecule biosynthetic process (Uniprot)
- GO:0040007 - growth (Uniprot)
- GO:0035250 - UDP-galactosyltransferase activity (Uniprot)
- GO:0016757 - transferase activity, transferring glycosyl groups (Uniprot)
- GO:0016740 - transferase activity (Uniprot)
- GO:0016021 - integral component of membrane (Uniprot)
- GO:0016020 - membrane (Uniprot)
- GO:0005886 - plasma membrane (Uniprot)
- GO:0005618 - cell wall (Uniprot)
- GO:0043022 - ribosome binding (FLUTE) (FLUTE Assigned!)
Reaction(s) (based on iSM810 metabolic model):
- ARAFDPP[c] + GALACTANDPP[c] <=> DPP[c] + ARA_91_1_93_GALACTANDPP[c]
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on aftA:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = -5.973 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology
- Photo of spot titer

Amino Acid Sequence

MPSRRKSPQFGHEMGAFTSARAREVLVALGQLAAAVVVAVGVAVVSLLAIARVEWPAFPSSNQLHALTTVGQVGCLAGLVGIGWLWRHGRFRRLARLGGL
VLVSAFTVVTLGMPLGATKLYLFGISVDQQFRTEYLTRLTDTAALRDMTYIGLPPFYPPGWFWIGGRAAALTGTPAWEMFKPWAITSMAIAVAVALVLWW
RMIRFEYALLVTVATAAVMLAYSSPEPYAAMITVLLPPMLVLTWSGLGARDRQGWAAVVGAGVFLGFAATWYTLLVAYGAFTVVLMALLLAGSRLQSGIK
AAVDPLCRLAVVGAIAAAIGSTTWLPYLLRAARDPVSDTGSAQHYLPADGAALTFPMLQFSLLGAICLLGTLWLVMRARSSAPAGALAIGVLAVYLWSLL
SMLATLARTTLLSFRLQPTLSVLLVAAGAFGFVEAVQALGKRGRGVIPMAAAIGLAGAIAFSQDIPDVLRPDLTIAYTDTDGYGQRGDRRPPGSEKYYPA
IDAAIRRVTGKRRDRTVVLTADYSFLSYYPYWGFQGLTPHYANPLAQFDKRATQIDSWSGLSTADEFIAALDKLPWQPPTVFLMRHGAHNSYTLRLAQDV
YPNQPNVRRYTVDLRTALFADPRFVVEDIGPFVLAIRKPQESA

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 33 non-insertions in a row out of 33 sites
Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 27 non-insertions in a row out of 33 sites
Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 1.000000; 33 non-insertions in a row out of 33 sites
Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 1.000000; 32 non-insertions in a row out of 33 sites
Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 1.000000; 32 non-insertions in a row out of 33 sites
Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.04
Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0023 (-)
- Rv3286c (sigF)

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- No bindings to other targets were found.

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Rv3286c (sigF)
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv0023 (-)

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv3792 (aftA)

Property	Value	Creator	Evidence	PMID	Comment
Citation	Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006	njamshidi	IDA	16595677	see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan
Term	TBRXN:AFTA arabinofuranosyl transferase (M tb) - IDA	njamshidi	IDA	16595677	see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
Citation	Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006	njamshidi	ISS	16595677	see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan
Term	TBRXN:AFTA arabinofuranosyl transferase (M tb) - ISS	njamshidi	ISS	16595677	see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
Name	Decaprenol phosphoarabinose-dependent arabinosyltransferase that transfers the first arabinosyl residue to the galactan backbone	mjackson	IDA		Arabinosyltransferases
Symbol	aftC	mjackson	IMP		Arabinosyltransferases
Name	Decaprenol phosphoarabinose-dependent arabinosyltransferase which transfers an arabinosyl unit to the 3 position of an arabinosyl residue	mjackson	IMP		Arabinosyltransferases
Symbol	aftC	mjackson	IDA		Arabinosyltransferases
Name	Decaprenol phosphoarabinose-dependent arabinosyltransferase which transfers an arabinosyl unit to the 3 position of an arabinosyl residue	mjackson	IDA		Arabinosyltransferases
Symbol	aftA	mjackson	IMP		Arabinosyltransferases
Name	Decaprenol phosphoarabinose-dependent arabinosyltransferase that transfers the first arabinosyl residue to the galactan backbone	mjackson	IMP		Arabinosyltransferases
Symbol	aftA	mjackson	IDA		Arabinosyltransferases
Symbol	aftC	mmcneil			Decaprenol phosphoarabinose-dependent arabinosyltransferase which transferase an arabinosyl unit to the 3 position of an arabinosyl residue HL. Birch, LJ. Alderwick et al. Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. Mol. Microbiol. 2008
Citation	Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. HL. Birch, LJ. Alderwick et al. Mol. Microbiol. 2008	mmcneil		18627460	Decaprenol phosphoarabinose-dependent arabinosyltransferase which transferase an arabinosyl unit to the 3 position of an arabinosyl residue
Term	GO:0052636 arabinosyltransferase activity - NR	mmcneil	NR		Decaprenol phosphoarabinose-dependent arabinosyltransferase which transferase an arabinosyl unit to the 3 position of an arabinosyl residue HL. Birch, LJ. Alderwick et al. Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. Mol. Microbiol. 2008
Symbol	afTA	mmcneil			Decaprenol phosphoarabinose-dependent arabinosyltransferase that transferase the first arabinosyl residue to the galactan backbone LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
Citation	Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006	mmcneil		16595677	Decaprenol phosphoarabinose-dependent arabinosyltransferase that transferase the first arabinosyl residue to the galactan backbone
Term	GO:0052636 arabinosyltransferase activity - NR	mmcneil	NR		Decaprenol phosphoarabinose-dependent arabinosyltransferase that transferase the first arabinosyl residue to the galactan backbone LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
Citation	Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. S. Berg, J. Starbuck et al. J. Biol. Chem. 2005	jjmcfadden		15546869	Inferred from direct assay
Term	EC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NR	jjmcfadden	NR		Inferred from direct assay S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005
Citation	Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. S. Berg, J. Starbuck et al. J. Biol. Chem. 2005	extern:JZUCKER		15546869	Inferred from direct assay
Term	EC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NR	extern:JZUCKER	NR		Inferred from direct assay S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005

Property

Value

Creator

Evidence

PMID

Comment

Citation

Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006

njamshidi

IDA

16595677

see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan

Term

TBRXN:AFTA arabinofuranosyl transferase (M tb) - IDA

njamshidi

IDA

16595677

see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006

Citation

Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006

njamshidi

ISS

16595677

see PMID: 16595677 and cole et al text (Tuberculosis and the tubercle bacillus) for more details. Priming reaction for subsequent addition of arabinan

Term

TBRXN:AFTA arabinofuranosyl transferase (M tb) - ISS

njamshidi

ISS

16595677

Name

Decaprenol phosphoarabinose-dependent arabinosyltransferase that transfers the first arabinosyl residue to the galactan backbone

mjackson

IDA

Arabinosyltransferases

Symbol

aftC

mjackson

IMP

Arabinosyltransferases

Name

Decaprenol phosphoarabinose-dependent arabinosyltransferase which transfers an arabinosyl unit to the 3 position of an arabinosyl residue

mjackson

IMP

Arabinosyltransferases

Symbol

aftC

mjackson

IDA

Arabinosyltransferases

Name

Decaprenol phosphoarabinose-dependent arabinosyltransferase which transfers an arabinosyl unit to the 3 position of an arabinosyl residue

mjackson

IDA

Arabinosyltransferases

Symbol

aftA

mjackson

IMP

Arabinosyltransferases

Name

Decaprenol phosphoarabinose-dependent arabinosyltransferase that transfers the first arabinosyl residue to the galactan backbone

mjackson

IMP

Arabinosyltransferases

Symbol

aftA

mjackson

IDA

Arabinosyltransferases

Symbol

aftC

mmcneil

Decaprenol phosphoarabinose-dependent arabinosyltransferase which transferase an arabinosyl unit to the 3 position of an arabinosyl residue
HL. Birch, LJ. Alderwick et al. Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. Mol. Microbiol. 2008

Citation

Biosynthesis of mycobacterial arabinogalactan: identification of a novel alpha(1-->3) arabinofuranosyltransferase. HL. Birch, LJ. Alderwick et al. Mol. Microbiol. 2008

mmcneil

18627460

Decaprenol phosphoarabinose-dependent arabinosyltransferase which transferase an arabinosyl unit to the 3 position of an arabinosyl residue

Term

GO:0052636 arabinosyltransferase activity - NR

mmcneil

Symbol

afTA

mmcneil

Decaprenol phosphoarabinose-dependent arabinosyltransferase that transferase the first arabinosyl residue to the galactan backbone
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006

Citation

Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. LJ. Alderwick, M. Seidel et al. J. Biol. Chem. 2006

mmcneil

16595677

Decaprenol phosphoarabinose-dependent arabinosyltransferase that transferase the first arabinosyl residue to the galactan backbone

Term

GO:0052636 arabinosyltransferase activity - NR

mmcneil

Citation

Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. S. Berg, J. Starbuck et al. J. Biol. Chem. 2005

jjmcfadden

15546869

Inferred from direct assay

Term

EC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NR

jjmcfadden

Inferred from direct assay
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005

Citation

Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. S. Berg, J. Starbuck et al. J. Biol. Chem. 2005

extern:JZUCKER

15546869

Inferred from direct assay

Term

EC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NR

extern:JZUCKER

Inferred from direct assay
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005

Comments