Rv3673c (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: hypothetical protein
- REFSEQ: membrane-anchored thioredoxin-like protein
- PATRIC: Possible membrane-anchored thioredoxin-like protein
- TUBERCULIST: Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein)
- NCBI: Possible membrane-anchored thioredoxin-like protein (thiol-disulfide interchange related protein)
- updated information (H37Rv4):
  - gene name: ccsX
  - function: cytochrome C maturation
  - reference: Small (2013) PMC3781833
Type: Essential
Start: 4114474
End: 4115157
Operon:

Trans-membrane region:
Role: I.B.7 - Miscellaneous oxidoreductases and oxygenases
GO terms:
- GO:0098869 - cellular oxidant detoxification (Uniprot)
- GO:0055114 - oxidation-reduction process (Uniprot)
- GO:0047134 - protein-disulfide reductase activity (Uniprot)
- GO:0045454 - cell redox homeostasis (Uniprot)
- GO:0034599 - cellular response to oxidative stress (Uniprot)
- GO:0016671 - oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor (Uniprot)
- GO:0016491 - oxidoreductase activity (Uniprot)
- GO:0016209 - antioxidant activity (Uniprot)
- GO:0016021 - integral component of membrane (Uniprot)
- GO:0016020 - membrane (Uniprot)
- GO:0015035 - protein disulfide oxidoreductase activity (Uniprot)
- GO:0006662 - glycerol ether metabolic process (Uniprot)
- GO:0005829 - cytosol (Uniprot)
- GO:0004791 - thioredoxin-disulfide reductase activity (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on Rv3673c:
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = -2.137 (from Jeremy Rock's lab)

Amino Acid Sequence

MPSLPTTPAETAMTTLTGKTRWTIAILAVVAALMAALVAQLHDYSASSTISQRPAPREHRDGDTPEALAWSRQRANLPPCPAAGNGPGAAALRGVVVVCA
GDGSAVDVARALAGRRVVINLWAHWCAPCMTELPVMAEYQRRVGPAVLVVTVHQGQNEAAALSRLADLGVRLPTLQDDRRRVAAALRVANVMPATVVLRP
DGSVAQTLPRAFGSADEIVAAVGNDAG

(Nucleotide sequence available on KEGG)

Additional Information

CcsX - thioredoxin-like membrane protein the inserts heme as part of cytochrome C maturation
Small et al (2013). mBio. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3781833/

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Uncertain	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.937400; 7 non-insertions in a row out of 7 sites
Uncertain	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.988100; 7 non-insertions in a row out of 7 sites
Uncertain	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.990500; 7 non-insertions in a row out of 7 sites
Uncertain	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.984000; 7 non-insertions in a row out of 7 sites
Uncertain	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.981900; 7 non-insertions in a row out of 7 sites
No-Data	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Too-Short	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: -1
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0818 (-)

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv1049 (-) (Direct binding)
- Rv0081 (-) (Upstream of operon)
- Rv0678 (-) (Upstream of operon)
- Rv3246c (mtrA) (Upstream of operon)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv0818 (-)

TB Genome Annotation Portal