TB Genome Annotation Portal

Rv3648c (cspA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: cold shock protein (beta-ribbon CspA family)
  • REFSEQ: cold shock protein A
  • PATRIC: Cold shock protein CspA
  • TUBERCULIST: Probable cold shock protein A CspA
  • NCBI: Probable cold shock protein A CspA
  • updated information (H37Rv4):
    • gene name: cspA
    • function:
    • reference:
• Type: Not Target
• Start: 4088328
• End: 4088531
• Operon:


• Trans-membrane region:
  
• Role: III.E - Adaptations and atypical conditions
• GO terms:
  • GO:0040007 - growth (Uniprot)
  • GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
  • GO:0006351 - transcription, DNA-templated (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0003677 - DNA binding (Uniprot)
  • GO:0003676 - nucleic acid binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5O6F	83%	Corynebacterium pseudotuberculosis	NMR structure of cold shock protein A from Corynebacterium pseudotuberculosis
  2I5M	64%	Bacillus subtilis	Crystal structure of Bacillus subtilis cold shock protein CspB variant A46K S48R
  1HZC	64%	Bacillus caldolyticus	BACILLUS CALDOLYTICUS COLD-SHOCK PROTEIN MUTANTS TO STUDY DETERMINANTS OF PROTEIN STABILITY
  1HZB	64%	Bacillus caldolyticus	BACILLUS CALDOLYTICUS COLD-SHOCK PROTEIN MUTANTS TO STUDY DETERMINANTS OF PROTEIN STABILITY
  1HZA	64%	Bacillus caldolyticus	BACILLUS CALDOLYTICUS COLD-SHOCK PROTEIN MUTANTS TO STUDY DETERMINANTS OF PROTEIN STABILITY
  3I2Z	63%	Salmonella typhimurium	Structure of cold shock protein E from Salmonella typhimurium
  2I5L	62%	Bacillus subtilis	Crystal structure of Bacillus subtilis Cold Shock Protein variant Bs-CspB M1R/E3K/K65I
  2HAX	62%	Bacillus caldolyticus	Crystal structure of Bacillus caldolyticus cold shock protein in complex with hexathymidine
  1I5F	62%	Bacillus caldolyticus	BACILLUS CALDOLYTICUS COLD-SHOCK PROTEIN MUTANTS TO STUDY DETERMINANTS OF PROTEIN STABILITY
  1HZ9	62%	Bacillus caldolyticus	BACILLUS CALDOLYTICUS COLD-SHOCK PROTEIN MUTANTS TO STUDY DETERMINANTS OF PROTEIN STABILITY

• Links to additional information on cspA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -3.923 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MPQGTVKWFNAEKGFGFIAPEDGSADVFVHYTEIQGTGFRTLEENQKVEFEIGHSPKGPQATGVRSL

Additional Information