TB Genome Annotation Portal

Rv3568c (hsaC)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: iron-dependent extradiol dioxygenase
  • REFSEQ: biphenyl-23-diol 12-dioxygenase
  • PATRIC: 23-dihydroxybiphenyl 12-dioxygenase (EC 1.13.11.39)
  • TUBERCULIST: 34-DHSA dioxygenase
  • NCBI: 3,4-DHSA dioxygenase
  • updated information (H37Rv4):
    • gene name: hsaC
    • function:
    • reference:
• Type: Conditional
• Start: 4009297
• End: 4010199
• Operon:


• Trans-membrane region:
  
• Role: I.B.7 - Miscellaneous oxidoreductases and oxygenases
• GO terms:
  • GO:0070723 - response to cholesterol (Uniprot)
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0051213 - dioxygenase activity (Uniprot)
  • GO:0047071 - 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione 4,5-dioxygenase activity (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0019439 - aromatic compound catabolic process (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0016042 - lipid catabolic process (Uniprot)
  • GO:0009405 - pathogenesis (Uniprot)
  • GO:0008203 - cholesterol metabolic process (Uniprot)
  • GO:0008202 - steroid metabolic process (Uniprot)
  • GO:0008198 - ferrous iron binding (Uniprot)
  • GO:0006725 - cellular aromatic compound metabolic process (Uniprot)
  • GO:0006707 - cholesterol catabolic process (Uniprot)
  • GO:0006629 - lipid metabolic process (Uniprot)
  • GO:0005506 - iron ion binding (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • 34DHSA[c] -> 49DSHA[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 2zi8, 2zyq
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2ZYQ	100%	Mycobacterium tuberculosis	Crystal structure of the HsaC extradiol dioxygenase from M. tuberculosis
  2ZI8	100%	Mycobacterium tuberculosis	Crystal structure of the HsaC extradiol dioxygenase from M. tuberculosis in complex with 3,4-dihydroxy-9,10-seconandrost-1,3,5(10)-triene-9,17-dione (DHSA)
  1LKD	39%	Burkholderia xenovorans	CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2',6'-DICL DIHYDROXYBIPHENYL (DHB)
  1LGT	39%	Burkholderia xenovorans	CRYSTAL STRUCTURE OF 2,3-DIHYDROXYBIPHENYL 1,2-DIOXYGENASE (DHBD) COMPLEXED WITH 2'-Cl DIHYDROXYBIPHENYL (DHB)
  1KNF	39%	Burkholderia xenovorans	Crystal Structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase Complexed with 3-methyl Catechol under Anaerobic Condition
  1KND	39%	Burkholderia xenovorans	Crystal Structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase Complexed with Catechol under Anaerobic Condition
  1KMY	39%	Burkholderia xenovorans	Crystal Structure of 2,3-dihydroxybiphenyl 1,2-dioxygenase Complexed with 2,3-dihydroxybiphenyl under Anaerobic Condition
  1HAN	39%	Burkholderia xenovorans	CRYSTAL STRUCTURE OF THE BIPHENYL-CLEAVING EXTRADIOL DIOXYGENASE FROM A PCB-DEGRADING PSEUDOMONAD
  1KW9	36%	Pseudomonas sp.	Crystal structure of 2,3-dihydroxybiphenyl dioxygenase (BphC) in complex with 2,3-dihydroxybiphenyl at 2.0A resolution
  1KW8	36%	Pseudomonas sp.	Crystal structure of BphC-2,3-dihydroxybiphenyl-NO complex

• Links to additional information on hsaC:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.932 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MSIRSLGYLRIEATDMAAWREYGLKVLGMVEGKGAPEGALYLRMDDFPARLVVVPGEHDRLLEAGWECANAEGLQEIRNRLDLEGTPYKEATAAELADRR
VDEMIRFADPSGNCLEVFHGTALEHRRVVSPYGHRFVTGEQGMGHVVLSTRDDAEALHFYRDVLGFRLRDSMRLPPQMVGRPADGPPAWLRFFGCNPRHH
SLAFLPMPTSSGIVHLMVEVEQADDVGLCLDRALRRKVPMSATLGRHVNDLMLSFYMKTPGGFDIEFGCEGRQVDDRDWIARESTAVSLWGHDFTVGARG

Additional Information