Rv3357 (relJ)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: antitoxin
- REFSEQ: hypothetical protein
- PATRIC: YefM protein (antitoxin to YoeB)
- TUBERCULIST: Antitoxin RelJ
- NCBI: Antitoxin RelJ
- updated information (H37Rv4):
  - gene name: relJ
  - function:
  - reference:
Type: Not Target
Start: 3770773
End: 3771048
Operon:

Trans-membrane region:
Role: V - Conserved hypotheticals
GO terms:
- GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
- GO:0006351 - transcription, DNA-templated (Uniprot)
- GO:0005515 - protein binding (Uniprot)
- GO:0003677 - DNA binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 3d55, 3cto, 3oei
Search for Homologs in PDB

Top 10 Homologs in PDB (as of Nov 2020):

PDB	aa ident	species	PDB title
3OEI	100%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis RelJK (Rv3357-Rv3358-RelBE3)
3OEI	100%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis RelJK (Rv3357-Rv3358-RelBE3)
3D55	100%	Mycobacterium tuberculosis	Crystal structure of M. tuberculosis YefM antitoxin
3CTO	100%	Mycobacterium tuberculosis	Crystal Structure of M. tuberculosis YefM antitoxin
2A6Q	50%	Escherichia coli	Crystal structure of YefM-YoeB complex
7BWF	38%	Staphylococcus aureus	YoeB-YefM complex from Staphylococcus aureus
7BWF	38%	Staphylococcus aureus	YoeB-YefM complex from Staphylococcus aureus
6L8F	38%	Staphylococcus aureus (strain NCTC 8325 / PS 47)	Crystal structure of heterotetrameric complex of YoeB-YefM toxin-antitoxin from Staphylococcus aureus.
6L8E	38%	Staphylococcus aureus subsp. aureus NCTC 8325	Crystal structure of heterohexameric YoeB-YefM complex bound to 26bp-DNA

Links to additional information on relJ:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.548 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSVDELREMAGGEE

(Nucleotide sequence available on KEGG)

Additional Information

Analysis of Positive Selection in Clinical Isolates new

Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
- Moldova: 2,057 clinical isolates
- global set: 5,195 clinical isolates from 15 other countries
In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	0.91 (0.07)	0.72 (0.25)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link

* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv3357/relJ, gene len: 275 bp, num TA sites: 5

condition	dataset	call	medium	method	notes
in-vitro	DeJesus 2017 mBio	non-essential	7H9	HMM	fully saturated, 14 TnSeq libraries combined
in-vitro	Sassetti 2003 Mol Micro	non-essential	7H9	TRASH	essential if hybridization ratio<0.2
in-vivo (mice)	Sassetti 2003 PNAS	non-essential	BL6 mice	TRASH	essential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)	Griffin 2011 PPath	non-essential	M9 minimal+glycerol	Gumbel	2 replicates; Padj<0.05
in-vitro (cholesterol)	Griffin 2011 PPath	non-essential	M9 minimal+cholesterol	Gumbel	3 replicates; Padj<0.05
differentially essential in cholesterol	Griffin 2011 PPath	NO (LFC=-0.13)	cholesterol vs glycerol	resampling-SR	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitro	Smith 2022 eLife	non-essential	7H9	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)	Smith 2022 eLife	non-essential	BL6 mice	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in mice	Smith 2022 eLife	NO (LFC=0.148)	in-vivo vs in-vitro	ZINB	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)	Minato 2019 mSys	non-essential	minimal medium	HMM
in-vitro (YM rich medium)	Minato 2019 mSys	non-essential	YM rich medium	HMM	7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich medium	Minato 2019 mSys	NO (LFC=-0.33)	YM rich vs minimal medium	resampling

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv1033c (trcR) (Direct binding)
- Rv1719 (-) (Direct binding)
- Rv2011c (-) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Rv0023 (-)
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv0260c (-)

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv3357 (relJ)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010
Citation	Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008	vashishtrv	IPI	19114484	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008
Citation	The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010	vashishtrv	IPI	20061486	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008
Citation	Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande J. Mol. Biol. 2008	shahanup86	IPI	18793646	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008
Citation	Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008	shahanup86	IPI	19114484	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008
Citation	The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010	shahanup86	IPI	20061486	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	shahanup86	IPI		Affinity purification (Physical interaction) authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010
Citation	Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande J. Mol. Biol. 2008	vashishtrv	IPI	18793646	Affinity purification (Physical interaction)
Interaction	PhysicalInteraction Rv3358	vashishtrv	IPI		Affinity purification (Physical interaction) authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008
Symbol	relJ	jlew			Each pair shows interaction by two-hybrid and GST pulldown. F and B each interact with E, G, K, and itself. authors,M. Yang,C. Gao,Y. Wang,H. Zhang,ZG. He Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules. PLoS ONE 2010
Citation	Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules. authors,M. Yang,C. Gao,Y. Wang,H. Zhang,ZG. He PLoS ONE 2010	jlew		20498855	Each pair shows interaction by two-hybrid and GST pulldown. F and B each interact with E, G, K, and itself.
Symbol	relB3	jlew			Each toxin individually arrests growth of both M. tuberculosis and E. coli, an effect that is neutralized by coexpression of the cognate antitoxin. authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010
Citation	The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010	jlew		20061486	Each toxin individually arrests growth of both M. tuberculosis and E. coli, an effect that is neutralized by coexpression of the cognate antitoxin.
Symbol	relJ	jlew			Overexpression of Mtb relE, relG and relK induces growth arrest in Msmeg; reversed by expression of relB, relF and relJ SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008
Citation	Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008	jlew		19114484	Overexpression of Mtb relE, relG and relK induces growth arrest in Msmeg; reversed by expression of relB, relF and relJ
Symbol	RelB3	jlew			We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin. authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009
Citation	Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009	jlew		19016878	We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin.
Symbol	yefM	jlew			Structure authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008
Citation	Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande J. Mol. Biol. 2008	jlew		18793646	Structure
Other	strand:+	rslayden			B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570
Symbol	rel BE-3	rslayden			SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 PUTATIVE PHD PROTEIN from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c
Other	start:3770773	rslayden			SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 PUTATIVE PHD PROTEIN from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c
Other	stop:3771048	rslayden			SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 PUTATIVE PHD PROTEIN from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c
Other	strand:+	rslayden			SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 PUTATIVE PHD PROTEIN from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c
Symbol	rel BE-3	rslayden			MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.
Other	start:3770773	rslayden			MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.
Other	stop:3771048	rslayden			MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.
Other	strand:+	rslayden			MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.
Symbol	rel BE-3	rslayden			Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7
Other	start:3770773	rslayden			Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7
Other	stop:3771048	rslayden			Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7
Other	strand:+	rslayden			Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7
Symbol	rel BE-3	rslayden			YU1E_STRCO (alias CAC37261
Other	start:3770773	rslayden			YU1E_STRCO (alias CAC37261
Other	stop:3771048	rslayden			YU1E_STRCO (alias CAC37261
Other	strand:+	rslayden			YU1E_STRCO (alias CAC37261
Symbol	rel BE-3	rslayden			SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147
Other	start:3770773	rslayden			SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147
Other	stop:3771048	rslayden			SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147
Other	strand:+	rslayden			SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147
Symbol	rel BE-3	rslayden			YEFM_ECOLI
Other	start:3770773	rslayden			YEFM_ECOLI
Other	stop:3771048	rslayden			YEFM_ECOLI
Other	strand:+	rslayden			YEFM_ECOLI
Symbol	rel BE-3	rslayden			B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570
Other	start:3770773	rslayden			B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570
Other	stop:3771048	rslayden			B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570

Property

Value

Creator

Evidence

PMID

Comment

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Affinity purification (Physical interaction)
authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Affinity purification (Physical interaction)
authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Affinity purification (Physical interaction)
SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Citation

Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008

vashishtrv

IPI

19114484

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Citation

The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010

vashishtrv

IPI

20061486

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Citation

Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande J. Mol. Biol. 2008

shahanup86

IPI

18793646

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Citation

Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008

shahanup86

IPI

19114484

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Citation

The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010

shahanup86

IPI

20061486

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

shahanup86

IPI

Citation

vashishtrv

IPI

18793646

Affinity purification (Physical interaction)

Interaction

PhysicalInteraction Rv3358

vashishtrv

IPI

Symbol

relJ

jlew

Each pair shows interaction by two-hybrid and GST pulldown. F and B each interact with E, G, K, and itself.
authors,M. Yang,C. Gao,Y. Wang,H. Zhang,ZG. He Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules. PLoS ONE 2010

Citation

Characterization of the interaction and cross-regulation of three Mycobacterium tuberculosis RelBE modules. authors,M. Yang,C. Gao,Y. Wang,H. Zhang,ZG. He PLoS ONE 2010

jlew

20498855

Each pair shows interaction by two-hybrid and GST pulldown. F and B each interact with E, G, K, and itself.

Symbol

relB3

jlew

Each toxin individually arrests growth of both M. tuberculosis and E. coli, an effect that is neutralized by coexpression of the cognate antitoxin.
authors,R. Singh,CE. Barry,HI. Boshoff The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. J. Bacteriol. 2010

Citation

The three RelE homologs of Mycobacterium tuberculosis have individual, drug-specific effects on bacterial antibiotic tolerance. authors,R. Singh,CE. Barry,HI. Boshoff J. Bacteriol. 2010

jlew

20061486

Each toxin individually arrests growth of both M. tuberculosis and E. coli, an effect that is neutralized by coexpression of the cognate antitoxin.

Symbol

relJ

jlew

Overexpression of Mtb relE, relG and relK induces growth arrest in Msmeg; reversed by expression of relB, relF and relJ
SB. Korch, H. Contreras et al. Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. J. Bacteriol. 2008

Citation

Three Mycobacterium tuberculosis Rel toxin:antitoxin modules inhibit mycobacterial growth and are expressed in human-infected macrophages. SB. Korch, H. Contreras et al. J. Bacteriol. 2008

jlew

19114484

Overexpression of Mtb relE, relG and relK induces growth arrest in Msmeg; reversed by expression of relB, relF and relJ

Symbol

RelB3

jlew

We report the heterologous toxicity of these TA loci in Escherichia coli and show that only a few of the M. tuberculosis-encoded toxins can inhibit E. coli growth and have a killing effect. This killing effect can be suppressed by coexpression of the cognate antitoxin.
authors,A. Gupta Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol. Lett. 2009

Citation

Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. authors,A. Gupta FEMS Microbiol. Lett. 2009

jlew

19016878

Symbol

yefM

jlew

Structure
authors,P. Kumar,B. Issac,EJ. Dodson,JP. Turkenburg,SC. Mande Crystal structure of Mycobacterium tuberculosis YefM antitoxin reveals that it is not an intrinsically unstructured protein. J. Mol. Biol. 2008

Citation

jlew

18793646

Structure

Other

strand:+

rslayden

B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570

Symbol

rel BE-3

rslayden

SAV2408 from Staphylococcus aureus subsp. aureus Mu50 (83 aa), FASTA scores: opt: 161, E(): 8.8e-05, (39.95% identity in 77 aa overlap); Q9Z5W8 PUTATIVE PHD PROTEIN from Francisella novicid (85 aa), FASTA scores: opt: 143, E(): 0.0016, (28.9% identity in 83 aa overlap); etc. Also similar to Rv1247c

Other

start:3770773

rslayden

Other

stop:3771048

rslayden

Other

strand:+

rslayden

Symbol

rel BE-3

rslayden

MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.

Other

start:3770773

rslayden

MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.

Other

stop:3771048

rslayden

MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.

Other

strand:+

rslayden

MTV006.19c (89 aa) (36.9% identity in 84 aa overlap). SEEMS TO BELONG TO THE PHD/YEFM FAMILY.

Symbol

rel BE-3

rslayden

Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7

Other

start:3770773

rslayden

Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7

Other

stop:3771048

rslayden

Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7

Other

strand:+

rslayden

Conserved hypothetical protein, highly similar to other hypothetical proteins e.g. Q9Z4V7

Symbol

rel BE-3

rslayden

YU1E_STRCO (alias CAC37261

Other

start:3770773

rslayden

YU1E_STRCO (alias CAC37261

Other

stop:3771048

rslayden

YU1E_STRCO (alias CAC37261

Other

strand:+

rslayden

YU1E_STRCO (alias CAC37261

Symbol

rel BE-3

rslayden

SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147

Other

start:3770773

rslayden

SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147

Other

stop:3771048

rslayden

SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147

Other

strand:+

rslayden

SCBAC17D6.02) ORFU1E (BELONGS TO THE PHD/YEFM FAMILY) from Streptomyces coelicolor (87 aa), FASTA scores: opt: 344, E(): 1.9e-17, (62.05% identity in 87 aa overlap); P46147

Symbol

rel BE-3

rslayden

YEFM_ECOLI

Other

start:3770773

rslayden

YEFM_ECOLI

Other

stop:3771048

rslayden

YEFM_ECOLI

Other

strand:+

rslayden

YEFM_ECOLI

Symbol

rel BE-3

rslayden

B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570

Other

start:3770773

rslayden

B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570

Other

stop:3771048

rslayden

B2017 from Escherichia coli strain K12 (83 aa), FASTA scores: opt: 215, E(): 1.6e-08, (50.0% identity in 72 aa overlap); BAB58570

Comments

TB Genome Annotation Portal

Rv3357 (relJ)

Amino Acid Sequence

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

Interactions based on ChIPSeq data

Interactions based on ChIPSeq data (Minch et al. 2014)

Interactions based on TFOE data (Rustad et al. 2014)

TBCAP

Comments

Analysis of Positive Selection in Clinical Isolates new