TB Genome Annotation Portal

Rv3251c (rubA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: rubredoxin 3
  • REFSEQ: rubredoxin RUBA
  • PATRIC: Rubredoxin
  • TUBERCULIST: Probable rubredoxin RubA
  • NCBI: Probable rubredoxin RubA
  • updated information (H37Rv4):
    • gene name: rubA
    • function:
    • reference:
• Type: Not Target
• Start: 3630571
• End: 3630738
• Operon:


• Trans-membrane region:
  
• Role: I.B.6.c - Electron transport
• GO terms:
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0005506 - iron ion binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2M4Y	83%	Mycobacterium ulcerans	Rubredoxin type protein from Mycobacterium ulcerans
  2V3B	56%	PSEUDOMONAS AERUGINOSA	Crystal structure of the electron transfer complex rubredoxin - rubredoxin reductase from Pseudomonas aeruginosa.
  1S24	54%	Pseudomonas oleovorans	Rubredoxin domain II from Pseudomonas oleovorans
  2KN9	54%	Mycobacterium tuberculosis	Solution structure of zinc-substituted rubredoxin B (Rv3250c) from Mycobacterium tuberculosis. Seattle Structural Genomics Center for Infectious Disease target MytuD.01635.a
  1RWD	48%	Pyrococcus furiosus	Backbone NMR Structure of a Mutant P. Furiosus Rubredoxin Using Residual Dipolar Couplings
  1IU6	48%	Pyrococcus furiosus	Neutron Crystal Structure of the rubredoxin mutant from Pyrococcus Furiosus
  1IU5	48%	Pyrococcus furiosus	X-ray Crystal Structure of the rubredoxin mutant from Pyrococcus Furiosus
  6NW1	47%	Desulfovibrio desulfuricans	Crystal Structure Desulfovibrio desulfuricans Nickel-Substituted Rubredoxin V37N
  5RXN	47%	Clostridium pasteurianum	COMBINED CRYSTALLOGRAPHIC REFINEMENT AND ENERGY MINIMIZATION OF RUBREDOXIN AT 1.2 ANGSTROM RESOLUTION
  4RXN	47%	Clostridium pasteurianum	CRYSTALLOGRAPHIC REFINEMENT OF RUBREDOXIN AT 1.2 ANGSTROMS RESOLUTION

• Links to additional information on rubA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.22 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MAAYRCPVCDYVYDEANGDAREGFPAGTGWDQIPDDWCCPDCAVREKVDFEKIGG

Additional Information