TB Genome Annotation Portal

Rv3246c (mtrA)

Amino Acid Sequence

MDTMRQRILVVDDDASLAEMLTIVLRGEGFDTAVIGDGTQALTAVRELRPDLVLLDLMLPGMNGIDVCRVLRADSGVPIVMLTAKTDTVDVVLGLESGAD
DYIMKPFKPKELVARVRARLRRNDDEPAEMLSIADVEIDVPAHKVTRNGEQISLTPLEFDLLVALARKPRQVFTRDVLLEQVWGYRHPADTRLVNVHVQR
LRAKVEKDPENPTVVLTVRGVGYKAGPP
(Nucleotide sequence available on KEGG)

Additional Information




Analysis of Positive Selection in Clinical Isolates *new*

Moldova (2,057)global set (5,195)
under significant positive selection?NONO
omega peak height (95%CI lower bound)0.63 (0.01)1.07 (0.21)
codons under selection
omega plots
genetic variants*linklink
statistics at each codonlinklink
* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"


ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv3246c/mtrA, gene len: 686 bp, num TA sites: 11
conditiondatasetcallmediummethodnotes
in-vitroDeJesus 2017 mBioessential7H9HMMfully saturated, 14 TnSeq libraries combined
in-vitroSassetti 2003 Mol Microno data 7H9TRASHessential if hybridization ratio<0.2
in-vivo (mice)Sassetti 2003 PNASessentialBL6 miceTRASHessential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)Griffin 2011 PPathessentialM9 minimal+glycerolGumbel2 replicates; Padj<0.05
in-vitro (cholesterol)Griffin 2011 PPathessentialM9 minimal+cholesterolGumbel3 replicates; Padj<0.05
differentially essential in cholesterol Griffin 2011 PPathNO (LFC=0.0)cholesterol vs glycerolresampling-SRYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitroSmith 2022 eLifeessential7H9HMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)Smith 2022 eLifeessentialBL6 miceHMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in miceSmith 2022 eLifeNO (LFC=0.0)in-vivo vs in-vitroZINBYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)Minato 2019 mSysgrowth defectminimal mediumHMM
in-vitro (YM rich medium)Minato 2019 mSysessentialYM rich mediumHMM7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich mediumMinato 2019 mSysNO (LFC=0.0)YM rich vs minimal mediumresampling

TnSeq Data No data currently available.
  • No TnSeq data currently available for this Target.
RNASeq Data No data currently available.
  • No RNA-Seq data currently available for this Target.
Metabolomic Profiles No data currently available.
  • No Metabolomic data currently available for this Target.
Proteomic Data No data currently available.
  • No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology
  • BioCyc

    Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

    NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

    Interactions based on ChIPSeq data

  • Interactions based on ChIPSeq data (Minch et al. 2014)

    Interactions based on TFOE data (Rustad et al. 2014)

    • Upregulates:

      • Does not upregulate other genes.
    • Upregulated by:

      • Not upregulated by other genes.
    • Downregulates:

      • Does not downregulate other genes.
    • Downregulated by:



    TBCAP

    Tubculosis Community Annotation Project (
    Slayden et al., 2013)

    Rv3246c (mtrA)

    PropertyValueCreatorEvidencePMIDComment
    CitationMycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. R. Sun, PJ. Converse et al. Mol. Microbiol. 2004sourish10IEP15049808Co-expression (Functional linkage)
    InteractionSignaling Rv2069sourish10IEPCo-expression (Functional linkage)
    R. Sun, PJ. Converse et al. Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. Mol. Microbiol. 2004
    InteractionSignaling Rv3245csourish10IPICo-expression (Functional linkage)
    TC. Zahrt & V. Deretic An essential two-component signal transduction system in Mycobacterium tuberculosis. J. Bacteriol. 2000
    InteractionSignaling Rv3245csourish10IPICo-expression (Functional linkage)
    KK. Singh, X. Zhang et al. Antigens of Mycobacterium tuberculosis expressed during preclinical tuberculosis: serological immunodominance of proteins with repetitive amino acid sequences. Infect. Immun. 2001
    CitationThe two-component regulatory system mtrAB is required for morphotypic multidrug resistance in Mycobacterium avium. authors,GA. Cangelosi,JS. Do,R. Freeman,JG. Bennett,M. Semret,MA. Behr Antimicrob. Agents Chemother. 2006sourish10TAS16436697Co-expression (Functional linkage)
    InteractionSignaling Rv3245csourish10TASCo-expression (Functional linkage)
    authors,GA. Cangelosi,JS. Do,R. Freeman,JG. Bennett,M. Semret,MA. Behr The two-component regulatory system mtrAB is required for morphotypic multidrug resistance in Mycobacterium avium. Antimicrob. Agents Chemother. 2006
    CitationAn essential two-component signal transduction system in Mycobacterium tuberculosis. TC. Zahrt & V. Deretic J. Bacteriol. 2000sourish10TAS10851001Co-expression (Functional linkage)
    InteractionSignaling Rv3245csourish10TASCo-expression (Functional linkage)
    TC. Zahrt & V. Deretic An essential two-component signal transduction system in Mycobacterium tuberculosis. J. Bacteriol. 2000
    CitationAntigens of Mycobacterium tuberculosis expressed during preclinical tuberculosis: serological immunodominance of proteins with repetitive amino acid sequences. KK. Singh, X. Zhang et al. Infect. Immun. 2001sourish10TAS11349098Co-expression (Functional linkage)
    InteractionSignaling Rv3245csourish10TASCo-expression (Functional linkage)
    KK. Singh, X. Zhang et al. Antigens of Mycobacterium tuberculosis expressed during preclinical tuberculosis: serological immunodominance of proteins with repetitive amino acid sequences. Infect. Immun. 2001
    InteractionRegulatory Rv3244charsharohiratruefriendIMPCo-expression (Functional linkage)
    N. Mker, M. Brocker et al. Deletion of the genes encoding the MtrA-MtrB two-component system of Corynebacterium glutamicum has a strong influence on cell morphology, antibiotics susceptibility and expression of genes involved in osmoprotection. Mol. Microbiol. 2004
    InteractionRegulatory Rv3244charsharohiratruefriendIMPCo-expression (Functional linkage)
    authors,PA. Hoskisson,MI. Hutchings MtrAB-LpqB: a conserved three-component system in actinobacteria? Trends Microbiol. 2006
    InteractionSignaling Rv3245csourish10IPICo-expression (Functional linkage)
    authors,GA. Cangelosi,JS. Do,R. Freeman,JG. Bennett,M. Semret,MA. Behr The two-component regulatory system mtrAB is required for morphotypic multidrug resistance in Mycobacterium avium. Antimicrob. Agents Chemother. 2006
    InteractionRegulatory Rv2069priti.prietyIEPCo-expression (Functional linkage)
    R. Sun, PJ. Converse et al. Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. Mol. Microbiol. 2004
    InteractionRegulatory Rv2069ashwinigbhatIEPCo-expression (Functional linkage)
    R. Sun, PJ. Converse et al. Mycobacterium tuberculosis ECF sigma factor sigC is required for lethality in mice and for the conditional expression of a defined gene set. Mol. Microbiol. 2004

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