TB Genome Annotation Portal

Rv3106 (fprA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: NADPH-ferredoxin reductase fprA
  • REFSEQ: NADPH:adrenodoxin oxidoreductase FPRA (NADPH-ferredoxin reductase)
  • PATRIC: Ferredoxin--NADP(+) reductase actinobacterial (eukaryote-like) type (EC 1.18.1.2)
  • TUBERCULIST: NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase)
  • NCBI: NADPH:adrenodoxin oxidoreductase FprA (NADPH-ferredoxin reductase)
  • updated information (H37Rv4):
    • gene name: fprA
    • function:
    • reference:
• Type: Not Target
• Start: 3474007
• End: 3475377
• Operon:


• Trans-membrane region:
  
• Role: I.B.6.c - Electron transport
• GO terms:
  • GO:0070401 - NADP+ binding (Uniprot)
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0050660 - flavin adenine dinucleotide binding (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0008860 - ferredoxin-NAD+ reductase activity (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0004324 - ferredoxin-NADP+ reductase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • NADP[c] + 2 FERO[c] <=> NADPH[c] + 2 FERI[c]
  • NADPH[c] + FE3[c] <=> NADP[c] + FE2[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 1lqu, 1lqt, 2c7g
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  1LQU	100%	Mycobacterium tuberculosis	Mycobacterium tuberculosis FprA in complex with NADPH
  1LQT	100%	Mycobacterium tuberculosis	A covalent modification of NADP+ revealed by the atomic resolution structure of FprA, a Mycobacterium tuberculosis oxidoreductase
  2C7G	99%	MYCOBACTERIUM TUBERCULOSIS	FprA from Mycobacterium tuberculosis: His57Gln mutant
  1E6E	40%	BOS TAURUS	ADRENODOXIN REDUCTASE/ADRENODOXIN COMPLEX OF MITOCHONDRIAL P450 SYSTEMS
  1E1N	40%	BOS TAURUS	Structure of adrenodoxin reductase at 2.4 Angstrom in crystal form A'
  1E1M	40%	BOVINE	ADRENODOXIN REDUCTASE in complex with NADPH obtained by a soaking experiment
  1E1L	40%	BOS TAURUS	STRUCTURE OF ADRENODOXIN REDUCTASE IN COMPLEX WITH NADP obtained by cocrystallisation
  1E1K	40%	BOVINE	ADRENODOXIN REDUCTASE in complex with NADP+ obtained by a soaking experiment
  1CJC	40%	Bos taurus	STRUCTURE OF ADRENODOXIN REDUCTASE OF MITOCHONDRIAL P450 SYSTEMS

• Links to additional information on fprA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.962 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MRPYYIAIVGSGPSAFFAAASLLKAADTTEDLDMAVDMLEMLPTPWGLVRSGVAPDHPKIKSISKQFEKTAEDPRFRFFGNVVVGEHVQPGELSERYDAV
IYAVGAQSDRMLNIPGEDLPGSIAAVDFVGWYNAHPHFEQVSPDLSGARAVVIGNGNVALDVARILLTDPDVLARTDIADHALESLRPRGIQEVVIVGRR
GPLQAAFTTLELRELADLDGVDVVIDPAELDGITDEDAAAVGKVCKQNIKVLRGYADREPRPGHRRMVFRFLTSPIEIKGKRKVERIVLGRNELVSDGSG
RVAAKDTGEREELPAQLVVRSVGYRGVPTPGLPFDDQSGTIPNVGGRINGSPNEYVVGWIKRGPTGVIGTNKKDAQDTVDTLIKNLGNAKEGAECKSFPE
DHADQVADWLAARQPKLVTSAHWQVIDAFERAAGEPHGRPRVKLASLAELLRIGLG

Additional Information