Rv3032 (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: hypothetical protein
- REFSEQ: transferase
- PATRIC: Glycosyl transferase group 1
- TUBERCULIST: Alpha (1->4) glucosyltransferase
- NCBI: Alpha (1->4) glucosyltransferase
- updated information (H37Rv4):
  - gene name: -
  - function: possible paralog of GlgA; glycogen synthase
  - reference: Koliwer-Brandl et al (2016) PMID 27513637
Type: Essential
Start: 3391534
End: 3392778
Operon:

Trans-membrane region:
Role: V - Conserved hypotheticals
GO terms:
- GO:0040007 - growth (Uniprot)
- GO:0016757 - transferase activity, transferring glycosyl groups (Uniprot)
- GO:0016740 - transferase activity (Uniprot)
- GO:0009103 - lipopolysaccharide biosynthetic process (Uniprot)
- GO:0005978 - glycogen biosynthetic process (Uniprot)
- GO:0005977 - glycogen metabolic process (Uniprot)
- GO:0005975 - carbohydrate metabolic process (Uniprot)
- GO:0004373 - glycogen (starch) synthase activity (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
- ADPGLC[c] -> H[c] + ADP[c] + LINEAR-GLUCAN[c]
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on Rv3032:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = -2.174 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MRILMVSWEYPPVVIGGLGRHVHHLSTALAAAGHDVVVLSRCPSGTDPSTHPSSDEVTEGVRVIAAAQDPHEFTFGNDMMAWTLAMGHAMIRAGLRLKKL
GTDRSWRPDVVHAHDWLVAHPAIALAQFYDVPMVSTIHATEAGRHSGWVSGALSRQVHAVESWLVRESDSLITCSASMNDEITELFGPGLAEITVIRNGI
DAARWPFAARRPRTGPAELLYVGRLEYEKGVHDAIAALPRLRRTHPGTTLTIAGEGTQQDWLIDQARKHRVLRATRFVGHLDHTELLALLHRADAAVLPS
HYEPFGLVALEAAAAGTPLVTSNIGGLGEAVINGQTGVSCAPRDVAGLAAAVRSVLDDPAAAQRRARAARQRLTSDFDWQTVATATAQVYLAAKRGERQP
QPRLPIVEHALPDR

(Nucleotide sequence available on KEGG)

Additional Information

possible paralog of GlgA, glycogen synthase

Analysis of Positive Selection in Clinical Isolates new

Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
- Moldova: 2,057 clinical isolates
- global set: 5,195 clinical isolates from 15 other countries
In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	2.54 (0.55)	1.52 (0.61)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link

* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv3032/-, gene len: 1244 bp, num TA sites: 11

condition	dataset	call	medium	method	notes
in-vitro	DeJesus 2017 mBio	growth defect	7H9	HMM	fully saturated, 14 TnSeq libraries combined
in-vitro	Sassetti 2003 Mol Micro	essential	7H9	TRASH	essential if hybridization ratio<0.2
in-vivo (mice)	Sassetti 2003 PNAS	no data	BL6 mice	TRASH	essential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)	Griffin 2011 PPath	essential	M9 minimal+glycerol	Gumbel	2 replicates; Padj<0.05
in-vitro (cholesterol)	Griffin 2011 PPath	essential	M9 minimal+cholesterol	Gumbel	3 replicates; Padj<0.05
differentially essential in cholesterol	Griffin 2011 PPath	NO (LFC=0.0)	cholesterol vs glycerol	resampling-SR	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitro	Smith 2022 eLife	essential	7H9	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)	Smith 2022 eLife	essential	BL6 mice	HMM	6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in mice	Smith 2022 eLife	NO (LFC=-0.047)	in-vivo vs in-vitro	ZINB	YES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)	Minato 2019 mSys	non-essential	minimal medium	HMM
in-vitro (YM rich medium)	Minato 2019 mSys	essential	YM rich medium	HMM	7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich medium	Minato 2019 mSys	NO (LFC=-3.03)	YM rich vs minimal medium	resampling

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0238 (-) (Upstream of operon)
- Rv2034 (-) (Upstream of operon)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:

Property	Value	Creator	Evidence	PMID	Comment
Name	alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis	mjackson	IMP		Other (lipo)polysaccharides [Methylglucose lipolysaccharides, glycogen and capsular alpha-1,4 glucan]
Name	alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis	mjackson	IDA		Other (lipo)polysaccharides [Methylglucose lipolysaccharides, glycogen and capsular alpha-1,4 glucan]
Other	TBPWY:(MGLP and alpha-1,4 glucans)	mjackson			alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic) T. Sambou, P. Dinadayala et al. Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice. Mol. Microbiol. 2008
Citation	Polymethylated polysaccharides from Mycobacterium species revisited. authors,M. Jackson,PJ. Brennan J. Biol. Chem. 2009	mjackson		18786916	alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic)
Other	TBPWY:(MGLP and alpha-1,4 glucans)	mjackson			alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic) authors,M. Jackson,PJ. Brennan Polymethylated polysaccharides from Mycobacterium species revisited. J. Biol. Chem. 2009
Citation	Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. G. Stadthagen, T. Sambou et al. J. Biol. Chem. 2007	mjackson		17640872	alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic)
Other	TBPWY:(MGLP and alpha-1,4 glucans)	mjackson			alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic) G. Stadthagen, T. Sambou et al. Genetic basis for the biosynthesis of methylglucose lipopolysaccharides in Mycobacterium tuberculosis. J. Biol. Chem. 2007
Citation	Capsular glucan and intracellular glycogen of Mycobacterium tuberculosis: biosynthesis and impact on the persistence in mice. T. Sambou, P. Dinadayala et al. Mol. Microbiol. 2008	mjackson		18808383	alpha-1,4-glucosyltransferase involved in glycogen, methylglucose lipopolysaccharides and capsular D-glucan synthesis (phenotypic [mycobacterial recombinant strains]; enzymatic)

TB Genome Annotation Portal