TB Genome Annotation Portal

Rv2958c (-)

Amino Acid Sequence

MEETSVAGDPGPDAGTSTAPNAAPEPVARRQRILFVGEAATLAHVVRPFVLARSLDPSRYEVHFACDPRFNKLLGPLPFPHHPIHTVPSEEVLLKIAQGR
LFYNTRTLRKYIAADRKILNEIAPDVVVGDNRLSLSVSARLAGIPYIAIANAYWSPQARRRFPLPDVPWTRFFGVRPVSILYRLYRPLIFALYCLPLNWL
RRKHGLSSLGWDLCRIFTDGDYTLYADVPELVPTYNLPANHRYLGPVLWSPDVKPPTWWHSLPTDRPIIYATLGSSGGKNLLQVVLNALADLPVTVIAAT
AGRNHLKNVPANAFVADYLPGEAAAARSAVVLCNGGSPTTQQALAAGVPVIGLPSNMDQHLNMEALERAGAGVLLRTERLNTEGVAAAVKQVLSGAEFRQ
AARRLAEAFGPDFAGFPQHIESALRLVC
(Nucleotide sequence available on KEGG)

Additional Information




Analysis of Positive Selection in Clinical Isolates *new*

Moldova (2,057)global set (5,195)
under significant positive selection?NONO
omega peak height (95%CI lower bound)1.78 (0.4)1.65 (0.79)
codons under selection
omega plots
genetic variants*linklink
statistics at each codonlinklink
* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"


ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv2958c/-, gene len: 1286 bp, num TA sites: 26
conditiondatasetcallmediummethodnotes
in-vitroDeJesus 2017 mBiogrowth advantage7H9HMMfully saturated, 14 TnSeq libraries combined
in-vitroSassetti 2003 Mol Micronon-essential 7H9TRASHessential if hybridization ratio<0.2
in-vivo (mice)Sassetti 2003 PNASnon-essential BL6 miceTRASHessential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)Griffin 2011 PPathnon-essentialM9 minimal+glycerolGumbel2 replicates; Padj<0.05
in-vitro (cholesterol)Griffin 2011 PPathnon-essentialM9 minimal+cholesterolGumbel3 replicates; Padj<0.05
differentially essential in cholesterol Griffin 2011 PPathNO (LFC=0.67)cholesterol vs glycerolresampling-SRYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitroSmith 2022 eLifenon-essential7H9HMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)Smith 2022 eLifenon-essentialBL6 miceHMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in miceSmith 2022 eLifeNO (LFC=0.208)in-vivo vs in-vitroZINBYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)Minato 2019 mSysnon-essentialminimal mediumHMM
in-vitro (YM rich medium)Minato 2019 mSysnon-essentialYM rich mediumHMM7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich mediumMinato 2019 mSysNO (LFC=0.46)YM rich vs minimal mediumresampling

TnSeq Data No data currently available.
  • No TnSeq data currently available for this Target.
RNASeq Data No data currently available.
  • No RNA-Seq data currently available for this Target.
Metabolomic Profiles No data currently available.
  • No Metabolomic data currently available for this Target.
Proteomic Data No data currently available.
  • No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology
  • BioCyc

    Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

    NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

    Interactions based on ChIPSeq data

  • Interactions based on ChIPSeq data (Minch et al. 2014)

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

    Interactions based on TFOE data (Rustad et al. 2014)



    TBCAP

    Tubculosis Community Annotation Project (
    Slayden et al., 2013)

    Rv2958c (-)

    PropertyValueCreatorEvidencePMIDComment
    TermTBRXN:RRPPDIMAS rhamnose rhamnose phenol phthiocerol dimycocerosate synthesis - IMPnjamshidiIMP15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    authors,J. Fldt,C. Dahlgren,A. Karlsson,AM. Ahmed,DE. Minnikin,M. Ridell Activation of human neutrophils by mycobacterial phenolic glycolipids. Clin. Exp. Immunol. 1999
    CitationCharacterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. E. Prez, P. Constant et al. J. Biol. Chem. 2004njamshidiISS15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    TermTBRXN:RRPPDIMAS rhamnose rhamnose phenol phthiocerol dimycocerosate synthesis - ISSnjamshidiISS15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    E. Prez, P. Constant et al. Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J. Biol. Chem. 2004
    CitationActivation of human neutrophils by mycobacterial phenolic glycolipids. authors,J. Fldt,C. Dahlgren,A. Karlsson,AM. Ahmed,DE. Minnikin,M. Ridell Clin. Exp. Immunol. 1999njamshidiISS15292272|10540187See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    TermTBRXN:RRPPDIMAS rhamnose rhamnose phenol phthiocerol dimycocerosate synthesis - ISSnjamshidiISS15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    authors,J. Fldt,C. Dahlgren,A. Karlsson,AM. Ahmed,DE. Minnikin,M. Ridell Activation of human neutrophils by mycobacterial phenolic glycolipids. Clin. Exp. Immunol. 1999
    CitationCharacterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. E. Prez, P. Constant et al. J. Biol. Chem. 2004njamshidiIMP15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    TermTBRXN:RRPPDIMAS rhamnose rhamnose phenol phthiocerol dimycocerosate synthesis - IMPnjamshidiIMP15292272See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    E. Prez, P. Constant et al. Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J. Biol. Chem. 2004
    CitationActivation of human neutrophils by mycobacterial phenolic glycolipids. authors,J. Fldt,C. Dahlgren,A. Karlsson,AM. Ahmed,DE. Minnikin,M. Ridell Clin. Exp. Immunol. 1999njamshidiIMP15292272|10540187See PMID: 15292272 and 10540187. Whether rhamnose or methoxy-ramnose is transfered is not yet clear, also the rhamnose donor has not been shown specifically to be dtdprmn
    InteractionOperon Rv2959cpriti.prietyIMPMethylation interface
    E. Prez, P. Constant et al. Molecular dissection of the role of two methyltransferases in the biosynthesis of phenolglycolipids and phthiocerol dimycoserosate in the Mycobacterium tuberculosis complex. J. Biol. Chem. 2004
    NameRhamnosyltransferase responsible for the transfer of the second rhamnosyl residue of the triglycosyl appendage of phenolic glycolipids and p-hydroxybenzoic acid derivativesmjacksonIMPPhthiocerol dimycocerosates (PDIM), phenolic glycolipids (PGL) and para-hydroxybenzoic acid derivatives
    CitationCharacterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. E. Prez, P. Constant et al. J. Biol. Chem. 2004mjackson15292272Rhamnosyltransferase responsible for the transfer of the second rhamnosyl residue of the triglycosyl appendage of phenolic glycolipids and p-hydroxybenzoic acid derivatives (phenotypic [mycobacterial recombinant strains])
    OtherTBPWY:Phthiocerol dimycocerosates, PGL & pHBADmjacksonRhamnosyltransferase responsible for the transfer of the second rhamnosyl residue of the triglycosyl appendage of phenolic glycolipids and p-hydroxybenzoic acid derivatives (phenotypic [mycobacterial recombinant strains])
    E. Prez, P. Constant et al. Characterization of three glycosyltransferases involved in the biosynthesis of the phenolic glycolipid antigens from the Mycobacterium tuberculosis complex. J. Biol. Chem. 2004

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