Rv2939 (papA5)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: phthiocerol/phthiodiolone dimycocerosyl transferase
- REFSEQ: acyltransferase PapA5
- PATRIC: Putative conserved polyketide synthase-associated protein PapA5
- TUBERCULIST: Possible conserved polyketide synthase associated protein PapA5
- NCBI: Possible conserved polyketide synthase associated protein PapA5
- updated information (H37Rv4):
  - gene name: papA5
  - function:
  - reference:
Type: Not Target
Start: 3274949
End: 3276217
Operon:

Trans-membrane region:
Role: I.I - Polyketide and non-ribosomal peptide synthesis
GO terms:
- GO:0071770 - DIM/DIP cell wall layer assembly (Uniprot)
- GO:0016747 - transferase activity, transferring acyl groups other than amino-acyl groups (Uniprot)
- GO:0016746 - transferase activity, transferring acyl groups (Uniprot)
- GO:0016740 - transferase activity (Uniprot)
- GO:0008610 - lipid biosynthetic process (Uniprot)
- GO:0008374 - O-acyltransferase activity (Uniprot)
- GO:0006629 - lipid metabolic process (Uniprot)
- GO:0005515 - protein binding (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
- 2 MYCOCEROSOYL_MAS[c] + PPTC[c] -> 2 MAS[c] + PHDIM_CYTO[c]
- 2 MYCOCEROSOYL_MAS[c] + PTC[c] -> 2 MAS[c] + DIM_CYTO[c]
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure: 1q9j
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020):

PDB aa ident species PDB title

1Q9J 100% Mycobacterium tuberculosis Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis
Links to additional information on papA5:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 1.329 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

PDB	aa ident	species	PDB title
1Q9J	100%	Mycobacterium tuberculosis	Structure of polyketide synthase associated protein 5 from Mycobacterium tuberculosis

Amino Acid Sequence

MFPGSVIRKLSHSEEVFAQYEVFTSMTIQLRGVIDVDALSDAFDALLETHPVLASHLEQSSDGGWNLVADDLLHSGICVIDGTAATNGSPSGNAELRLDQ
SVSLLHLQLILREGGAELTLYLHHCMADGHHGAVLVDELFSRYTDAVTTGDPGPITPQPTPLSMEAVLAQRGIRKQGLSGAERFMSVMYAYEIPATETPA
VLAHPGLPQAVPVTRLWLSKQQTSDLMAFGREHRLSLNAVVAAAILLTEWQLRNTPHVPIPYVYPVDLRFVLAPPVAPTEATNLLGAASYLAEIGPNTDI
VDLASDIVATLRADLANGVIQQSGLHFGTAFEGTPPGLPPLVFCTDATSFPTMRTPPGLEIEDIKGQFYCSISVPLDLYSCAVYAGQLIIEHHGHIAEPG
KSLEAIRSLLCTVPSEYGWIME

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 29 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 2 non-insertions in a row out of 29 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 2 non-insertions in a row out of 29 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 29 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 3 non-insertions in a row out of 29 sites
Non-Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Non-Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 0.61
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0827c (kmtR)

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0081 (-) (Direct binding)
- Rv0678 (-) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
- Not upregulated by other genes.
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Rv0827c (kmtR)

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv2939 (papA5)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	PhysicalInteraction Rv2935	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2931	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2932	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2934	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2933	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2936	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2937	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2938	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2933	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2936	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2937	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2938	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2935	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Citation	Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Nature 1999	shahanup86	TAS	10573420	Operon (Functional linkage)
Interaction	PhysicalInteraction Rv2930	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	Regulatory Rv3416	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Interaction	Regulatory Rv3692	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Citation	Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. LR. Camacho, P. Constant et al. J. Biol. Chem. 2001	shahanup86	TAS	11279114	Operon (Functional linkage)
Interaction	PhysicalInteraction Rv2930	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2931	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2932	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2934	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	Regulatory Rv2745c	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	Regulatory Rv3416	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	Regulatory Rv3692	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Citation	Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. DM. Collins, B. Skou et al. Infect. Immun. 2005	ashwinigbhat	IDA	15784584	One hybrid System
Interaction	Regulatory Rv0117	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Interaction	Regulatory Rv0445c	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Interaction	Regulatory Rv2034	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Interaction	Regulatory Rv2745c	ashwinigbhat	IDA		One hybrid System DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005
Citation	Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. M. Guo, H. Feng et al. Genome Res. 2009	ashwinigbhat	IDA	19228590	One hybrid System
Interaction	Regulatory Rv0117	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	Regulatory Rv0445c	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	Regulatory Rv2034	ashwinigbhat	IDA		One hybrid System M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	PhysicalInteraction Rv2935	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2935	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2934	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2934	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2933	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2933	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2932	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2932	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2931	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2931	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	PhysicalInteraction Rv2930	shahanup86	TAS		Operon (Functional linkage) authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999
Interaction	PhysicalInteraction Rv2930	shahanup86	TAS		Operon (Functional linkage) LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001
Interaction	Signaling Rv0019c	vmevada102	IDA		Spectrophotometric Analysis M. Gupta,A. Sajid,G. Arora,V. Tandon,Y. Singh Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis. J. Biol. Chem. 2009
Interaction	RegulatedBy Rv3692	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv3416	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv2745c	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv1956	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv1359	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv0445c	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Interaction	RegulatedBy Rv0117	yamir.moreno	IDA		One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. . M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009
Name	Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates	mjackson	IMP		Phthiocerol dimycocerosates (PDIM), phenolic glycolipids (PGL) and para-hydroxybenzoic acid derivatives
Name	Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates	mjackson	IDA		Phthiocerol dimycocerosates (PDIM), phenolic glycolipids (PGL) and para-hydroxybenzoic acid derivatives
Citation	Dissecting the mechanism and assembly of a complex virulence mycobacterial lipid. OA. Trivedi, P. Arora et al. Mol. Cell 2005	mjackson		15749014	Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
Other	TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD	mjackson			Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic) OA. Trivedi, P. Arora et al. Dissecting the mechanism and assembly of a complex virulence mycobacterial lipid. Mol. Cell 2005
Citation	Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5. KC. Onwueme, JA. Ferreras et al. Proc. Natl. Acad. Sci. U.S.A. 2004	mjackson		15070765	Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
Other	TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD	mjackson			Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic) KC. Onwueme, JA. Ferreras et al. Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5. Proc. Natl. Acad. Sci. U.S.A. 2004
Citation	Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J. Buglino, KC. Onwueme et al. J. Biol. Chem. 2004	mjackson		15123643	Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
Other	TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD	mjackson			Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic) J. Buglino, KC. Onwueme et al. Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J. Biol. Chem. 2004

Property

Value

Creator

Evidence

PMID

Comment

Interaction

PhysicalInteraction Rv2935

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2931

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2932

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2934

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2933

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2936

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2937

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2938

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2933

shahanup86

TAS

Operon (Functional linkage)
LR. Camacho, P. Constant et al. Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. J. Biol. Chem. 2001

Interaction

PhysicalInteraction Rv2936

shahanup86

TAS

Interaction

PhysicalInteraction Rv2937

shahanup86

TAS

Interaction

PhysicalInteraction Rv2938

shahanup86

TAS

Interaction

PhysicalInteraction Rv2935

shahanup86

TAS

Citation

Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Nature 1999

shahanup86

TAS

10573420

Operon (Functional linkage)

Interaction

PhysicalInteraction Rv2930

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

Regulatory Rv3416

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Interaction

Regulatory Rv3692

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Citation

Analysis of the phthiocerol dimycocerosate locus of Mycobacterium tuberculosis. Evidence that this lipid is involved in the cell wall permeability barrier. LR. Camacho, P. Constant et al. J. Biol. Chem. 2001

shahanup86

TAS

11279114

Operon (Functional linkage)

Interaction

PhysicalInteraction Rv2930

shahanup86

TAS

Interaction

PhysicalInteraction Rv2931

shahanup86

TAS

Interaction

PhysicalInteraction Rv2932

shahanup86

TAS

Interaction

PhysicalInteraction Rv2934

shahanup86

TAS

Interaction

Regulatory Rv2745c

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

Regulatory Rv3416

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

Regulatory Rv3692

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Citation

Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. DM. Collins, B. Skou et al. Infect. Immun. 2005

ashwinigbhat

IDA

15784584

One hybrid System

Interaction

Regulatory Rv0117

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Interaction

Regulatory Rv0445c

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Interaction

Regulatory Rv2034

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Interaction

Regulatory Rv2745c

ashwinigbhat

IDA

One hybrid System
DM. Collins, B. Skou et al. Generation of attenuated Mycobacterium bovis strains by signature-tagged mutagenesis for discovery of novel vaccine candidates. Infect. Immun. 2005

Citation

Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. M. Guo, H. Feng et al. Genome Res. 2009

ashwinigbhat

IDA

19228590

One hybrid System

Interaction

Regulatory Rv0117

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

Regulatory Rv0445c

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

Regulatory Rv2034

ashwinigbhat

IDA

One hybrid System
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

PhysicalInteraction Rv2935

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2935

shahanup86

TAS

Interaction

PhysicalInteraction Rv2934

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2934

shahanup86

TAS

Interaction

PhysicalInteraction Rv2933

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2933

shahanup86

TAS

Interaction

PhysicalInteraction Rv2932

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2932

shahanup86

TAS

Interaction

PhysicalInteraction Rv2931

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2931

shahanup86

TAS

Interaction

PhysicalInteraction Rv2930

shahanup86

TAS

Operon (Functional linkage)
authors,JS. Cox,B. Chen,M. McNeil,WR. Jacobs Complex lipid determines tissue-specific replication of Mycobacterium tuberculosis in mice. Nature 1999

Interaction

PhysicalInteraction Rv2930

shahanup86

TAS

Interaction

Signaling Rv0019c

vmevada102

IDA

Spectrophotometric Analysis
M. Gupta,A. Sajid,G. Arora,V. Tandon,Y. Singh Forkhead-associated domain-containing protein Rv0019c and polyketide-associated protein PapA5, from substrates of serine/threonine protein kinase PknB to interacting proteins of Mycobacterium tuberculosis. J. Biol. Chem. 2009

Interaction

RegulatedBy Rv3692

yamir.moreno

IDA

One hybrid reporter system. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
M. Guo, H. Feng et al. Dissecting transcription regulatory pathways through a new bacterial one-hybrid reporter system. Genome Res. 2009

Interaction

RegulatedBy Rv3416

yamir.moreno

IDA

Interaction

RegulatedBy Rv2745c

yamir.moreno

IDA

Interaction

RegulatedBy Rv1956

yamir.moreno

IDA

Interaction

RegulatedBy Rv1359

yamir.moreno

IDA

Interaction

RegulatedBy Rv0445c

yamir.moreno

IDA

Interaction

RegulatedBy Rv0117

yamir.moreno

IDA

Name

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates

mjackson

IMP

Phthiocerol dimycocerosates (PDIM), phenolic glycolipids (PGL) and para-hydroxybenzoic acid derivatives

Name

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates

mjackson

IDA

Phthiocerol dimycocerosates (PDIM), phenolic glycolipids (PGL) and para-hydroxybenzoic acid derivatives

Citation

Dissecting the mechanism and assembly of a complex virulence mycobacterial lipid. OA. Trivedi, P. Arora et al. Mol. Cell 2005

mjackson

15749014

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)

Other

TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD

mjackson

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
OA. Trivedi, P. Arora et al. Dissecting the mechanism and assembly of a complex virulence mycobacterial lipid. Mol. Cell 2005

Citation

Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5. KC. Onwueme, JA. Ferreras et al. Proc. Natl. Acad. Sci. U.S.A. 2004

mjackson

15070765

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)

Other

TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD

mjackson

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
KC. Onwueme, JA. Ferreras et al. Mycobacterial polyketide-associated proteins are acyltransferases: proof of principle with Mycobacterium tuberculosis PapA5. Proc. Natl. Acad. Sci. U.S.A. 2004

Citation

Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J. Buglino, KC. Onwueme et al. J. Biol. Chem. 2004

mjackson

15123643

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)

Other

TBPWY:Phthiocerol dimycocerosates, PGL & pHBAD

mjackson

Acyltransferase responsible for the transfer of mycocerosic acids to phthiocerol to form phthiocerol dimycocerosates (phenotypic [mycobacterial recombinant strains]; enzymatic)
J. Buglino, KC. Onwueme et al. Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J. Biol. Chem. 2004

Comments