TB Genome Annotation Portal

Rv2913c (-)

Amino Acid Sequence

MLAWRQLNDLEETVTYDVIIRDGLWFDGTGNAPLTRTLGIRDGVVATVAAGALDETGCPEVVDAAGKWVVPGFIDVHTHYDAEVLLDPGLRESVRHGVTT
VLLGNCSLSTVYANSEDAADLFSRVEAVPREFVLGALRDNQTWSTPAEYIEAIDALPLGPNVSSLLGHSDLRTAVLGLDRATDDTVRPTEAELAKMAKLL
DEALEAGMLGMSGMDAAIDKLDGDRFRSRALPSTFATWRERRKLISVLRHRGRILQSAPDVDNPVSALLFFLASSRIFNRRKGVRMSMLVSADAKSMPLA
VHVFGLGTRVLNKLLGSQVRFQHLPVPFELYSDGIDLPVFEEFGAGTAALHLRDQLQRNELLADRSYRRSFRREFDRIKLGPSLWHRDFHDAVIVECPDK
SLIGKSFGAIADERGLHPLDAFLDVLVDNGERNVRWTTIVANHRPNQLNKLAAEPSVHMGFSDAGAHLRNMAFYNFGLRLLKRARDADRAGQPFLSIERA
VYRLTGELAEWFGIGAGTLRQGDRADFAVIDPTHLDESVDGYHEEAVPYYGGLRRMVNRNDATVVATGVGGTVVFRGGQFGGQFRDGYGQNVKSGRYLRA
GELGAALSRSA
(Nucleotide sequence available on KEGG)

Additional Information




Analysis of Positive Selection in Clinical Isolates *new*

Moldova (2,057)global set (5,195)
under significant positive selection?NONO
omega peak height (95%CI lower bound)1.93 (0.4)1.73 (0.55)
codons under selection
omega plots
genetic variants*linklink
statistics at each codonlinklink
* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"


ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv2913c/-, gene len: 1835 bp, num TA sites: 30
conditiondatasetcallmediummethodnotes
in-vitroDeJesus 2017 mBionon-essential7H9HMMfully saturated, 14 TnSeq libraries combined
in-vitroSassetti 2003 Mol Micronon-essential 7H9TRASHessential if hybridization ratio<0.2
in-vivo (mice)Sassetti 2003 PNASnon-essential BL6 miceTRASHessential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)Griffin 2011 PPathuncertainM9 minimal+glycerolGumbel2 replicates; Padj<0.05
in-vitro (cholesterol)Griffin 2011 PPathuncertainM9 minimal+cholesterolGumbel3 replicates; Padj<0.05
differentially essential in cholesterol Griffin 2011 PPathNO (LFC=0.46)cholesterol vs glycerolresampling-SRYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitroSmith 2022 eLifenon-essential7H9HMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)Smith 2022 eLifenon-essentialBL6 miceHMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in miceSmith 2022 eLifeNO (LFC=-0.044)in-vivo vs in-vitroZINBYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)Minato 2019 mSysnon-essentialminimal mediumHMM
in-vitro (YM rich medium)Minato 2019 mSysnon-essentialYM rich mediumHMM7H9 supplemented with ~20 metabolites (amino acids, cofactors)
differentially essential in YM rich mediumMinato 2019 mSysNO (LFC=-0.21)YM rich vs minimal mediumresampling

TnSeq Data No data currently available.
  • No TnSeq data currently available for this Target.
RNASeq Data No data currently available.
  • No RNA-Seq data currently available for this Target.
Metabolomic Profiles No data currently available.
  • No Metabolomic data currently available for this Target.
Proteomic Data No data currently available.
  • No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology
  • BioCyc

    Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

    NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

    Interactions based on ChIPSeq data

  • Interactions based on ChIPSeq data (Minch et al. 2014)

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

    Interactions based on TFOE data (Rustad et al. 2014)



    TBCAP

    Tubculosis Community Annotation Project (
    Slayden et al., 2013)

    Rv2913c (-)

    PropertyValueCreatorEvidencePMIDComment
    InteractionSignaling Rv2914cpriti.prietyIDAAffinity purification (Physical interaction)
    R. Gopalaswamy, PR. Narayanan et al. Cloning, overexpression, and characterization of a serine/threonine protein kinase pknI from Mycobacterium tuberculosis H37Rv. Protein Expr. Purif. 2004
    InteractionSignaling Rv2914cpriti.prietyIDAAffinity purification (Physical interaction)
    R. Gopalaswamy, S. Narayanan et al. The serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiol. Lett. 2009
    InteractionSignaling Rv2914cpriti.prietyIDAAffinity purification (Physical interaction)
    A. Singh, Y. Singh et al. Protein kinase I of Mycobacterium tuberculosis: cellular localization and expression during infection of macrophage-like cells. Tuberculosis (Edinburgh, Scotland) 2006
    InteractionOperon Rv2916cashwinigbhatNASCo-expression (Functional linkage)
    authors,BM. Altura Role of prostaglandins and histamine in reactive hyperemia: in-vivo studies on single mesenteric arterioles. Prostaglandins Med 1978
    CitationMeso-appendicular testis. authors,A. Zelikovski,J. Abu-Dalu,I. Urca Br J Urol 1975ashwinigbhatNAS122Co-expression (Functional linkage)
    InteractionOperon Rv2914cashwinigbhatNASCo-expression (Functional linkage)
    authors,A. Zelikovski,J. Abu-Dalu,I. Urca Meso-appendicular testis. Br J Urol 1975
    InteractionOperon Rv2916cashwinigbhatNASCo-expression (Functional linkage)
    authors,A. Zelikovski,J. Abu-Dalu,I. Urca Meso-appendicular testis. Br J Urol 1975
    CitationThe eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Y. Av-Gay,M. Everett Trends Microbiol. 2000ashwinigbhatNAS10785641Co-expression (Functional linkage)
    InteractionOperon Rv2914cashwinigbhatNASCo-expression (Functional linkage)
    Y. Av-Gay,M. Everett The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Trends Microbiol. 2000
    InteractionOperon Rv2916cashwinigbhatNASCo-expression (Functional linkage)
    Y. Av-Gay,M. Everett The eukaryotic-like Ser/Thr protein kinases of Mycobacterium tuberculosis. Trends Microbiol. 2000
    CitationThe serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. R. Gopalaswamy, S. Narayanan et al. FEMS Microbiol. Lett. 2009ashwinigbhatNAS19341393Co-expression (Functional linkage)
    InteractionOperon Rv2914cashwinigbhatNASCo-expression (Functional linkage)
    R. Gopalaswamy, S. Narayanan et al. The serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiol. Lett. 2009
    InteractionOperon Rv2916cashwinigbhatNASCo-expression (Functional linkage)
    R. Gopalaswamy, S. Narayanan et al. The serine/threonine protein kinase PknI controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiol. Lett. 2009
    CitationRole of prostaglandins and histamine in reactive hyperemia: in-vivo studies on single mesenteric arterioles. authors,BM. Altura Prostaglandins Med 1978ashwinigbhatNAS102004Co-expression (Functional linkage)
    InteractionOperon Rv2914cashwinigbhatNASCo-expression (Functional linkage)
    authors,BM. Altura Role of prostaglandins and histamine in reactive hyperemia: in-vivo studies on single mesenteric arterioles. Prostaglandins Med 1978

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