TB Genome Annotation Portal

Rv2833c (ugpB)

Amino Acid Sequence

MDPLNRRQFLALAAAAAGVTAGCAGMGGGGSVKSGSGPIDFWSSHPGQSSAAERELIGRFQDRFPTLSVKLIDAGKDYDEVAQKFNAALIGTDVPDVVLL
DDRWWFHFALSGVLTALDDLFGQVGVDTTDYVDSLLADYEFNGRHYAVPYARSTPLFYYNKAAWQQAGLPDRGPQSWSEFDEWGPELQRVVGAGRSAHGW
ANADLISWTFQGPNWAFGGAYSDKWTLTLTEPATIAAGNFYRNSIHGKGYAAVANDIANEFATGILASAVASTGSLAGITASARFDFGAAPLPTGPDAAP
ACPTGGAGLAIPAKLSEERKVNALKFIAFVTNPTNTAYFSQQTGYLPVRKSAVDDASERHYLADNPRARVALDQLPHTRTQDYARVFLPGGDRIISAGLE
SIGLRGADVTKTFTNIQKRLQVILDRQIMRKLAGHG
(Nucleotide sequence available on KEGG)

Additional Information




Analysis of Positive Selection in Clinical Isolates *new*

Moldova (2,057)global set (5,195)
under significant positive selection?NONO
omega peak height (95%CI lower bound)1.96 (0.55)1.9 (0.87)
codons under selection
omega plots
genetic variants*linklink
statistics at each codonlinklink
* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"


ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv2833c/ugpB, gene len: 1310 bp, num TA sites: 20
conditiondatasetcallmediummethodnotes
in-vitroDeJesus 2017 mBionon-essential7H9HMMfully saturated, 14 TnSeq libraries combined
in-vitroSassetti 2003 Mol Microessential 7H9TRASHessential if hybridization ratio<0.2
in-vivo (mice)Sassetti 2003 PNASnon-essential BL6 miceTRASHessential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)Griffin 2011 PPathnon-essentialM9 minimal+glycerolGumbel2 replicates; Padj<0.05
in-vitro (cholesterol)Griffin 2011 PPathnon-essentialM9 minimal+cholesterolGumbel3 replicates; Padj<0.05
differentially essential in cholesterol Griffin 2011 PPathNO (LFC=1.48)cholesterol vs glycerolresampling-SRYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitroSmith 2022 eLifenon-essential7H9HMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)Smith 2022 eLifenon-essentialBL6 miceHMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in miceSmith 2022 eLifeNO (LFC=0.25)in-vivo vs in-vitroZINBYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)Minato 2019 mSysnon-essentialminimal mediumHMM
in-vitro (YM rich medium)Minato 2019 mSysnon-essentialYM rich mediumHMM7H9 supplemented with ~20 metabolites (amino acids, vitamins)
differentially essential in YM rich mediumMinato 2019 mSysNO (LFC=-0.34)YM rich vs minimal mediumresampling

TnSeq Data No data currently available.
  • No TnSeq data currently available for this Target.
RNASeq Data No data currently available.
  • No RNA-Seq data currently available for this Target.
Metabolomic Profiles No data currently available.
  • No Metabolomic data currently available for this Target.
Proteomic Data No data currently available.
  • No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology
  • BioCyc

    Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

    NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

    Interactions based on ChIPSeq data

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

    Interactions based on ChIPSeq data (Minch et al. 2014)

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

      • No bindings to other targets were found.

    Interactions based on TFOE data (Rustad et al. 2014)

    • Upregulates:

      • Does not upregulate other genes.
    • Upregulated by:

    • Downregulates:

      • Does not downregulate other genes.
    • Downregulated by:

      • Not downregulated by other genes.


    TBCAP

    Tubculosis Community Annotation Project (
    Slayden et al., 2013)

    Rv2833c (ugpB)

    PropertyValueCreatorEvidencePMIDComment
    TermTBRXN:GLYC3Pabc sn-Glycerol 3-phosphate transport via ABC system - ISSnjamshidiISS12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    authors,CM. Sassetti,DH. Boyd,EJ. Rubin Genes required for mycobacterial growth defined by high density mutagenesis. Mol. Microbiol. 2003
    CitationThe ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. authors,M. Braibant,P. Gilot,J. Content FEMS Microbiol. Rev. 2000njamshidiISS10978546|12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    TermTBRXN:GLYC3Pabc sn-Glycerol 3-phosphate transport via ABC system - ISSnjamshidiISS12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    authors,M. Braibant,P. Gilot,J. Content The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. FEMS Microbiol. Rev. 2000
    CitationGenes required for mycobacterial growth defined by high density mutagenesis. authors,CM. Sassetti,DH. Boyd,EJ. Rubin Mol. Microbiol. 2003njamshidiIPI12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    TermTBRXN:GLYC3Pabc sn-Glycerol 3-phosphate transport via ABC system - IPInjamshidiIPI12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    authors,CM. Sassetti,DH. Boyd,EJ. Rubin Genes required for mycobacterial growth defined by high density mutagenesis. Mol. Microbiol. 2003
    CitationThe ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. authors,M. Braibant,P. Gilot,J. Content FEMS Microbiol. Rev. 2000njamshidiIPI10978546|12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    TermTBRXN:GLYC3Pabc sn-Glycerol 3-phosphate transport via ABC system - IPInjamshidiIPI12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    authors,M. Braibant,P. Gilot,J. Content The ATP binding cassette (ABC) transport systems of Mycobacterium tuberculosis. FEMS Microbiol. Rev. 2000
    CitationGenes required for mycobacterial growth defined by high density mutagenesis. authors,CM. Sassetti,DH. Boyd,EJ. Rubin Mol. Microbiol. 2003njamshidiISS12657046Believed to be importer exp evidence PMID: 12657046. See also PMID: 10978546. Cole et al: Tuberculosis and the Tubercle Bacillus chapter 24 - unknown mechism. high affinity for basic amino acids; unknown mechanism, for current version assume permease/diffusion transport
    SymbolUgpBjlewContains a functional tat sequence. Test for functional tat sequences using a BlaC reporter. 13 identified.
    JA. McDonough, JR. McCann et al. Identification of functional Tat signal sequences in Mycobacterium tuberculosis proteins. J. Bacteriol. 2008
    CitationIdentification of functional Tat signal sequences in Mycobacterium tuberculosis proteins. JA. McDonough, JR. McCann et al. J. Bacteriol. 2008jlew18658266Contains a functional tat sequence. Test for functional tat sequences using a BlaC reporter. 13 identified.

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