TB Genome Annotation Portal

Rv2710 (sigB)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: RNA polymerase sigma factor sigB
  • REFSEQ: RNA polymerase sigma factor SigB
  • PATRIC: RNA polymerase sigma factor SigB
  • TUBERCULIST: RNA polymerase sigma factor SigB
  • NCBI: RNA polymerase sigma factor SigB
  • updated information (H37Rv4):
    • gene name: sigB
    • function:
    • reference:
• Type: Not Target
• Start: 3022461
• End: 3023432
• Operon:


• Trans-membrane region:
  
• Role: II.A.7 - RNA synthesis, RNA modification and DNA transcription
• GO terms:
  • GO:2000142 - regulation of DNA-templated transcription, initiation (Uniprot)
  • GO:0050708 - regulation of protein secretion (Uniprot)
  • GO:0045893 - positive regulation of transcription, DNA-templated (Uniprot)
  • GO:0043175 - RNA polymerase core enzyme binding (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0019217 - regulation of fatty acid metabolic process (Uniprot)
  • GO:0016987 - bacterial sigma factor activity (Uniprot)
  • GO:0009410 - response to xenobiotic stimulus (Uniprot)
  • GO:0009408 - response to heat (Uniprot)
  • GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
  • GO:0006352 - DNA-templated transcription, initiation (Uniprot)
  • GO:0006351 - transcription, DNA-templated (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0003700 - DNA binding transcription factor activity (Uniprot)
  • GO:0003677 - DNA binding (Uniprot)
  • GO:0001666 - response to hypoxia (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3LEV	75%	Thermus aquaticus	HIV-1 antibody 2F5 in complex with epitope scaffold ES2
  3LES	75%	Thermus aquaticus	2F5 Epitope scaffold ES2
  4X8K	73%	Mycobacterium tuberculosis	Mycobacterium tuberculosis RbpA-SID in complex with SigmaA domain 2
  1SIG	65%	Escherichia coli	CRYSTAL STRUCTURE OF A SIGMA70 SUBUNIT FRAGMENT FROM ESCHERICHIA COLI RNA POLYMERASE
  5YIX	64%	Caulobacter crescentus (strain NA1000 / CB15N)	Caulobacter crescentus GcrA sigma-interacting domain (SID) in complex with domain 2 of sigma 70
  5H6X	63%	Legionella pneumophila	The crystal structure of RpoS fragment including a partial region 1.2 and region 2 from intracellular pathogen Legionella pneumophila
  6VW0	62%	Mycobacterium tuberculosis	Mycobacterium tuberculosis RNAP S456L mutant open promoter complex
  6VVZ	62%	Mycobacterium tuberculosis	Mycobacterium tuberculosis RNAP S456L mutant transcription initiation intermediate structure with Sorangicin
  6VVY	62%	Mycobacterium tuberculosis	Mycobacterium tuberculosis WT RNAP transcription open promoter complex with Sorangicin
  6VVX	62%	Mycobacterium tuberculosis	Mycobacterium tuberculosis WT RNAP transcription initiation intermediate structure with Sorangicin

• Links to additional information on sigB:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -0.989 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MADAPTRATTSRVDSDLDAQSPAADLVRVYLNGIGKTALLNAAGEVELAKRIEAGLYAEHLLETRKRLGENRKRDLAAVVRDGEAARRHLLEANLRLVVS
LAKRYTGRGMPLLDLIQEGNLGLIRAMEKFDYTKGFKFSTYATWWIRQAITRGMADQSRTIRLPVHLVEQVNKLARIKREMHQHLGREATDEELAAESGI
PIDKINDLLEHSRDPVSLDMPVGSEEEAPLGDFIEDAEAMSAENAVIAELLHTDIRSVLATLDEREHQVIRLRFGLDDGQPRTLDQIGKLFGLSRERVRQ
IERDVMSKLRHGERADRLRSYAS

Additional Information