TB Genome Annotation Portal

Rv2691 (ceoB)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: trk system potassium uptake protein TrkA
  • REFSEQ: TRK system potassium uptake protein CEOB
  • PATRIC: Trk system potassium uptake protein TrkA
  • TUBERCULIST: TRK system potassium uptake protein CeoB
  • NCBI: TRK system potassium uptake protein CeoB
  • updated information (H37Rv4):
    • gene name: ceoB
    • function:
    • reference:
• Coordinates in H37Rv: 3009344 - 3010027
• Gene length: 684 bp (with stop codon), 227 aa (without stop codon)
• Operon:


• Trans-membrane region:
  
• Role: III.A.2 - Cations
• GO terms:
  • GO:0098655 - cation transmembrane transport (Uniprot)
  • GO:0071805 - potassium ion transmembrane transport (Uniprot)
  • GO:0015079 - potassium ion transmembrane transporter activity (Uniprot)
  • GO:0008324 - cation transmembrane transporter activity (Uniprot)
  • GO:0006813 - potassium ion transport (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6VPB	40%	Gluconacetobacter diazotrophicus (strain ATCC 49037 / DSM 5601 / PAl5)	A novel membrane-bound 6-phosphogluconate dehydrogenase from the acetic acid bacteria Gluconacetobacter diazotrophicus (Gd6PGD)
  3L4B	35%	Thermotoga maritima	Crystal Structure of an Octomeric Two-Subunit TrkA K+ Channel Ring Gating Assembly, TM1088A:TM1088B, from Thermotoga maritima
  2G1U	35%	Thermotoga maritima	CRYSTAL STRUCTURE OF A PUTATIVE TRANSPORT PROTEIN (TM1088A) FROM THERMOTOGA MARITIMA AT 1.50 A RESOLUTION

• Links to additional information on ceoB:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -0.604 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MRVVVMGCGRVGASVADGLSRIGHEVAIIDRDSAAFNRLSPQFAGERVLGQGFDRDVLLRAGIQGADAFAAVSSGDNSNIISARLARETFGVPRVVARIY
DAKRAEVYERLGIPTITTVPWTTDRLLNALMQDTETAKWRDPTGTVAVAEVVLHEDWVGHRATDLEQATGARIAFLIRFGTGVLPEPKTVLQAGDKVYIA
AISGRAAEAAAIAALPPSEDFESGARR

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• data from Culviner et al (2025) (55,259 Mtb clinical isolates)
  • overall pN/pS for Rv2691: 0.981858995
  • lineage-specific pN/pS in L1: 0.823422657
  • lineage-specific pN/pS in L2: 0.525260232
  • lineage-specific pN/pS in L3: 1.071118904
  • lineage-specific pN/pS in L4: 1.258564712

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	YES
omega peak height (95%CI lower bound)	1.07 (0.23)	2.71 (1.26)
codons under selection		118, 119, 120, 121, 122, 123, 124, 125, 126, 127, 128, 129, 130, 131, 132, 133, 134
omega plots
genetic variants*	link	link
statistics at each codon	link	link