TB Genome Annotation Portal

Rv2605c (tesB2)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: acyl-CoA thioesterase II
  • REFSEQ: acyl-CoA thioesterase II
  • PATRIC: Acyl-CoA thioesterase II (EC 3.1.2.-)
  • TUBERCULIST: Probable acyl-CoA thioesterase II TesB2 (TEII)
  • NCBI: Probable acyl-CoA thioesterase II TesB2 (TEII)
  • updated information (H37Rv4):
    • gene name: tesB2
    • function:
    • reference:
• Type: Not Target
• Start: 2932297
• End: 2933142
• Operon:


• Trans-membrane region:
  
• Role: I.H.1 - Synthesis of fatty and mycolic acids
• GO terms:
  • GO:0047617 - acyl-CoA hydrolase activity (Uniprot)
  • GO:0009062 - fatty acid catabolic process (Uniprot)
  • GO:0006637 - acyl-CoA metabolic process (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • FORCOA[c] -> H[c] + FOR[c] + COA[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3U0A	85%	Mycobacterium marinum	Crystal structure of an Acyl-CoA thioesterase II TesB2 from Mycobacterium marinum
  1C8U	41%	Escherichia coli	CRYSTAL STRUCTURE OF THE E.COLI THIOESTERASE II, A HOMOLOGUE OF THE HUMAN NEF-BINDING ENZYME
  4R4U	41%	Yersinia pestis	Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis in complex with coenzyme A
  4QFW	41%	Yersinia pestis	Crystal structure of acyl-CoA thioesterase tesB from Yersinia pestis
  4R9Z	35%	Mycobacterium avium subsp. paratuberculosis	Mycobacterium avium subs paratuberculosis tesB protein MAP1729c
  3RD7	35%	Mycobacterium avium	Crystal structure of acyl-coa thioesterase from mycobacterium avium

• Links to additional information on tesB2:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.316 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VSIEEILDLEQLEVNIYRGSVFSPESGFLQRTFGGHVAGQSLVSAVRTVDPRYMVHSLHGYFLRPGDAKERTVFLVERIRDGGSFCTRRVNAVQHGETIF
SMAASFQTEQEGITHQDVMPAAPPPDGLPGLNSIKVFDDAGFRQFDEWDVCIVPRERLRLLPGKASQQQVWLRHRDPLPDDPVLHICALAYMSDLTLLGS
AQVNHLDVRDQLQVASLDHAMWFMRPFRADEWLLYDQSSPSASGGRALTRGEIFTRSGEMVAAVMQEGLTRHRRGHRSVGQ

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	1.71 (0.26)	0.93 (0.39)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link