TB Genome Annotation Portal

Rv2593c (ruvA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: Holliday junction ATP-dependent DNA helicase ruvA
  • REFSEQ: Holliday junction DNA helicase RuvA
  • PATRIC: Holliday junction DNA helicase RuvA
  • TUBERCULIST: Probable holliday junction DNA helicase RuvA
  • NCBI: Probable holliday junction DNA helicase RuvA
  • updated information (H37Rv4):
    • gene name: ruvA
    • function:
    • reference:
• Type: Not Target
• Start: 2924230
• End: 2924820
• Operon:


• Trans-membrane region:
  
• Role: II.A.5 - DNA replication, repair, recombination and restriction/modification
• GO terms:
  • GO:0032508 - DNA duplex unwinding (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0009432 - SOS response (Uniprot)
  • GO:0009379 - Holliday junction helicase complex (Uniprot)
  • GO:0009378 - four-way junction helicase activity (Uniprot)
  • GO:0006974 - cellular response to DNA damage stimulus (Uniprot)
  • GO:0006310 - DNA recombination (Uniprot)
  • GO:0006281 - DNA repair (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0004386 - helicase activity (Uniprot)
  • GO:0003678 - DNA helicase activity (Uniprot)
  • GO:0003677 - DNA binding (Uniprot)
  • GO:0000724 - double-strand break repair via homologous recombination (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 2ztc, 2zte, 2h5x, 2ztd
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2ZTE	100%	Mycobacterium tuberculosis	MtRuvA Form IV
  2ZTD	100%	Mycobacterium tuberculosis	MtRuvA Form III
  2ZTC	100%	Mycobacterium tuberculosis	MtRuvA Form II
  2H5X	100%	Mycobacterium tuberculosis	RuvA from Mycobacterium tuberculosis
  1BVS	75%	Mycobacterium leprae	RUVA COMPLEXED TO A HOLLIDAY JUNCTION.
  1D8L	39%	Escherichia coli	E. COLI HOLLIDAY JUNCTION BINDING PROTEIN RUVA NH2 REGION LACKING DOMAIN III
  1CUK	39%	Escherichia coli	ESCHERICHIA COLI RUVA PROTEIN AT PH 4.9 AND ROOM TEMPERATURE
  1IXR	37%	Thermus thermophilus	RuvA-RuvB complex

• Links to additional information on ruvA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -2.543 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MIASVRGEVLEVALDHVVIEAAGVGYRVNATPATLATLRQGTEARLITAMIVREDSMTLYGFPDGETRDLFLTLLSVSGVGPRLAMAALAVHDAPALRQV
LADGNVAALTRVPGIGKRGAERMVLELRDKVGVAATGGALSTNGHAVRSPVVEALVGLGFAAKQAEEATDTVLAANHDATTSSALRSALSLLGKAR

Additional Information