TB Genome Annotation Portal

Rv2500c (fadE19)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: acyl-CoA dehydrogenase fadE19
  • REFSEQ: acyl-CoA dehydrogenase
  • PATRIC: Isovaleryl-CoA dehydrogenase (EC 1.3.99.10)
  • TUBERCULIST: Possible acyl-CoA dehydrogenase FadE19 (MMGC)
  • NCBI: Possible acyl-CoA dehydrogenase FadE19 (MMGC)
  • updated information (H37Rv4):
    • gene name: fadE19
    • function:
    • reference:
• Type: Essential
• Start: 2813727
• End: 2814911
• Operon:


• Trans-membrane region:
  
• Role: I.A.3 - Fatty acids
• GO terms:
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0050660 - flavin adenine dinucleotide binding (Uniprot)
  • GO:0016627 - oxidoreductase activity, acting on the CH-CH group of donors (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0003995 - acyl-CoA dehydrogenase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5OL2	46%	Clostridioides difficile	The electron transferring flavoprotein/butyryl-CoA dehydrogenase complex from Clostridium difficile
  4U83	44%	Brucella abortus	Structure of Brucella Abortus Butyryl-CoA dehydrogenase
  1JQI	43%	Rattus norvegicus	Crystal Structure of Rat Short Chain Acyl-CoA Dehydrogenase Complexed With Acetoacetyl-CoA
  4M9A	43%	Burkholderia thailandensis	Crystal structure of Acyl-coA dehydrogenase from Burkholderia thailandensis E264
  4L1F	43%	Acidaminococcus fermentans	Electron transferring flavoprotein of Acidaminococcus fermentans: Towards a mechanism of flavin-based electron bifurcation
  5LNX	43%	Bacillus subtilis (strain 168)	Crystal structure of MmgC, an acyl-CoA dehydrogenase from bacillus subtilis.
  1BUC	42%	Megasphaera elsdenii	THREE-DIMENSIONAL STRUCTURE OF BUTYRYL-COA DEHYDROGENASE FROM MEGASPHAERA ELSDENII
  4N5F	42%	Burkholderia cenocepacia	Crystal Structure of a Putative acyl-CoA dehydrogenase with bound FADH2 from Burkholderia cenocepacia J2315
  2VIG	42%	HOMO SAPIENS	Crystal structure of human short-chain acyl CoA dehydrogenase
  2D29	39%	Thermus thermophilus	Structural study on project ID TT0172 from Thermus thermophilus HB8

• Links to additional information on fadE19:
  • KEGG - nucleotide and amino acid sequences of gene
  • BioCyc
  • Systems Biology
  • Mycobrowser
  • PATRIC
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.668 (from Jeremy Rock's lab)

Amino Acid Sequence

MTTTTTTISGGILPKEYQDLRDTVADFARTVVAPVSAKHDAEHSFPYEIVAKMGEMGLFGLPFPEEYGGMGGDYFALSLVLEELGKVDQSVAITLEAAVG
LGAMPIYRFGTEEQKQKWLPDLTSGRALAGFGLTEPGAGSDAGSTRTTARLEGDEWIINGSKQFITNSGTDITSLVTVTAVTGTTGTAADAKKEISTIIV
PSGTPGFTVEPVYNKVGWNASDTHPLTFADARVPRENLLGARGSGYANFLSILDEGRIAIAALATGAAQGCVDESVKYANQRQSFGQPIGAYQAIGFKIA
RMEARAHVARTAYYDAAAKMLAGKPFKKEAAIAKMISSEAAMDNSRDATQIHGGYGFMNEYPVARHYRDSKVLEIGEGTTEVQLMLIARSLGLQ

Additional Information