TB Genome Annotation Portal

Rv2400c (subI)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: sulfate ABC transporter sulfate-binding protein
  • REFSEQ: sulfate-binding lipoprotein
  • PATRIC: Sulfate and thiosulfate binding protein CysP
  • TUBERCULIST: Probable sulfate-binding lipoprotein SubI
  • NCBI: Probable sulfate-binding lipoprotein SubI
  • updated information (H37Rv4):
    • gene name: subI
    • function:
    • reference:
• Type: Not Target
• Start: 2696644
• End: 2697714
• Operon:


• Trans-membrane region:
  
• Role: III.A.4 - Anions
• GO terms:
  • GO:1902358 - sulfate transmembrane transport (Uniprot)
  • GO:0099133 - ATP hydrolysis coupled anion transmembrane transport (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0015419 - ATPase-coupled sulfate transmembrane transporter activity (Uniprot)
  • GO:0008272 - sulfate transport (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0000103 - sulfate assimilation (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ATP[c] -> ADP[c] + PI[c] + SLF[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6DDN	94%	Mycobacterium tuberculosis	The sulfate-binding protein SubI from Mycobacterium tuberculosis H37Rv
  1SBP	36%	Salmonella typhimurium	1.7 ANGSTROMS REFINED STRUCTURE OF SULFATE-BINDING PROTEIN INVOLVED IN ACTIVE TRANSPORT AND NOVEL MODE OF SULFATE BINDING
  5UM2	36%	Xanthomonas axonopodis pv. citri	Functional and structural characterization of a Sulfate-binding protein (Sbp) from Xanthomonas citri

• Links to additional information on subI:
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -1.496 (from Jeremy Rock's lab)

Amino Acid Sequence

MLSLTLSEASCIASASRWRHIIPAGVVCALIAGIGVGCHGGPSDVVGRAGPDRAHTSITLVAYAVPEPGWSAVIPAFNASEQGRGVQVITSYGASADQSR
GVADGKPADLVNFSVEPDIARLVKAGKVDKDWDADATKGIPFGSVVTFVVRAGNPKNIRDWDDLLRPGIEVITPSPLSSGSAKWNLLAPYAAKSDGGRNN
QAGIDFVNTLVNEHVKLRPGSGREATDVFVQGSGDVLISYENEAIATERAGKPVQHVTPPQTFKIENPLAVVATSTHLGAATAFRNFQYTVQAQKLWAQA
GFRPVDPAVAADFADLFPVPAKLWTIADLGGWGSVDPQLFDKATGSITKIYLRATG

Additional Information