TB Genome Annotation Portal

Rv2247 (accD6)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: propionyl-CoA carboxylase beta chain
  • REFSEQ: acetyl/propionyl-CoA carboxylase beta subunit AccD6
  • PATRIC: Propionyl-CoA carboxylase beta chain (EC 6.4.1.3)
  • TUBERCULIST: Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6
  • NCBI: Acetyl/propionyl-CoA carboxylase (beta subunit) AccD6
  • updated information (H37Rv4):
    • gene name: accD6
    • function:
    • reference:
• Type: Essential
• Start: 2520743
• End: 2522164
• Operon:


• Trans-membrane region:
  
• Role: I.H.1 - Synthesis of fatty and mycolic acids
• GO terms:
  • GO:0040007 - growth (Uniprot)
  • GO:0019367 - fatty acid elongation, saturated fatty acid (Uniprot)
  • GO:0016874 - ligase activity (Uniprot)
  • GO:0009317 - acetyl-CoA carboxylase complex (Uniprot)
  • GO:0009062 - fatty acid catabolic process (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0004658 - propionyl-CoA carboxylase activity (Uniprot)
  • GO:0003989 - acetyl-CoA carboxylase activity (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • PROPIONYLCOA[c] + BIOTIN_CO2[c] -> SMMALONYLCOA[c] + 0.999 BIOTIN[c]
  • BIOTIN_CO2[c] + 3MBECOA[c] -> 0.999 BIOTIN[c] + MGLUTCOA[c]
  • ACCOA[c] + BIOTIN_CO2[c] -> 0.999 BIOTIN[c] + MALCOA[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6TZV	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal Structure of the carboxyltransferase subunit of ACC (AccD6) in complex with inhibitor Phenyl-Cyclodiaone from Mycobacterium tuberculosis
  6PRW	100%	Mycobacterium tuberculosis	CRYSTAL STRUCTURE OF THE CARBOXYLTRANSFERASE SUBUNIT OF ACC (ACCD6) IN COMPLEX WITH INHIBITOR QUIZALOFOP-P DERIVATIVE FROM MYCOBACTERIUM TUBERCULOSIS
  6PK2	100%	Mycobacterium tuberculosis	CRYSTAL STRUCTURE OF THE CARBOXYLTRANSFERASE SUBUNIT OF ACC (ACCD6) IN COMPLEX WITH INHIBITOR QUIZALOFOP-P derivative FROM MYCOBACTERIUM TUBERCULOSIS
  6P7U	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	CRYSTAL STRUCTURE OF THE CARBOXYLTRANSFERASE SUBUNIT OF ACC (ACCD6) IN COMPLEX WITH INHIBITOR QUIZALOFOP-P FROM MYCOBACTERIUM TUBERCULOSIS
  4L6W	100%	Mycobacterium tuberculosis	Carboxyltransferase subunit (AccD6) of Mycobacterium tuberculosis acetyl-CoA carboxylase
  4G2R	100%	Mycobacterium tuberculosis	Crystal Structure of the carboxyltransferase subunit of ACC (AccD6) in complex with inhibitor haloxyfop from Mycobacterium tuberculosis
  4FB8	100%	Mycobacterium tuberculosis	Crystal Structure of apo Acyl-CoA Carboxylase
  3N6R	43%	Roseobacter denitrificans	CRYSTAL STRUCTURE OF the holoenzyme of PROPIONYL-COA CARBOXYLASE (PCC)
  2A7S	43%	Mycobacterium tuberculosis	Crystal Structure of the Acyl-CoA Carboxylase, AccD5, from Mycobacterium tuberculosis
  1XNW	43%	Streptomyces coelicolor	Acyl-CoA Carboxylase Beta Subunit from S. coelicolor (PccB), apo form #2, mutant D422I

• Links to additional information on accD6:
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -11.453 (from Jeremy Rock's lab)

Amino Acid Sequence

MTIMAPEAVGESLDPRDPLLRLSNFFDDGSVELLHERDRSGVLAAAGTVNGVRTIAFCTDGTVMGGAMGVEGCTHIVNAYDTAIEDQSPIVGIWHSGGAR
LAEGVRALHAVGQVFEAMIRASGYIPQISVVVGFAAGGAAYGPALTDVVVMAPESRVFVTGPDVVRSVTGEDVDMASLGGPETHHKKSGVCHIVADDELD
AYDRGRRLVGLFCQQGHFDRSKAEAGDTDIHALLPESSRRAYDVRPIVTAILDADTPFDEFQANWAPSMVVGLGRLSGRTVGVLANNPLRLGGCLNSESA
EKAARFVRLCDAFGIPLVVVVDVPGYLPGVDQEWGGVVRRGAKLLHAFGECTVPRVTLVTRKTYGGAYIAMNSRSLNATKVFAWPDAEVAVMGAKAAVGI
LHKKKLAAAPEHEREALHDQLAAEHERIAGGVDSALDIGVVDEKIDPAHTRSKLTEALAQAPARRGRHKNIPL

Additional Information