TB Genome Annotation Portal

Rv2234 (ptpA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: protein-tyrosine phosphatase
  • REFSEQ: phosphotyrosine protein phosphatase PTPA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase)
  • PATRIC: Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48)
  • TUBERCULIST: Phosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase)
  • NCBI: Phosphotyrosine protein phosphatase PtpA (protein-tyrosine-phosphatase) (PTPase) (LMW phosphatase)
  • updated information (H37Rv4):
    • gene name: ptpA
    • function:
    • reference:
• Type: Not Target
• Start: 2507146
• End: 2507637
• Operon:


• Trans-membrane region:
  
• Role: I.J.3 - Serine-Threonine protein kinases and phosphoprotein phosphatases
• GO terms:
  • GO:0052083 - negative regulation by symbiont of host cell-mediated immune response (Uniprot)
  • GO:0052046 - modification by symbiont of host morphology or physiology via secreted substance (Uniprot)
  • GO:0044174 - host cell endosome (Uniprot)
  • GO:0035335 - peptidyl-tyrosine dephosphorylation (Uniprot)
  • GO:0030430 - host cell cytoplasm (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0009405 - pathogenesis (Uniprot)
  • GO:0006470 - protein dephosphorylation (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005576 - extracellular region (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0004725 - protein tyrosine phosphatase activity (Uniprot)
  • GO:0004721 - phosphoprotein phosphatase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 1u2q, 1u2p, 2luo
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  2LUO	99%	Mycobacterium tuberculosis	NMR solution structure of apo-MptpA
  1U2Q	99%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis Low Molecular Weight Protein Tyrosine Phosphatase (MPtpA) at 2.5A resolution with glycerol in the active site
  1U2P	99%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis Low Molecular Protein Tyrosine Phosphatase (MPtpA) at 1.9A resolution
  2CWD	44%	Thermus thermophilus	Crystal Structure of TT1001 protein from Thermus thermophilus HB8
  5O7B	38%	Synechocystis sp. (strain PCC 6803 / Kazusa)	CRYSTAL STRUCTURE OF THE SLR0328 TYROSINE PHOSPHATASE WZB FROM SYNECHOCYSTIS SP. PCC 6803
  1XWW	38%	Homo sapiens	Crystal Structure of Human B-form Low Molecular Weight Phosphotyrosyl Phosphatase at 1.6 Angstrom Resolution
  6Y2W	37%	Homo sapiens	Crystal structure of the single mutant I16K of Low Molecular Weight Protein Tyrosine Phosphatase (LMW-PTP)
  6Y2V	37%	Homo sapiens	Crystal structure of the double mutant L13R I16K of Low Molecular Weight Protein Tyrosine Phosphatase (LMW-PTP)
  5PNT	37%	Homo sapiens	CRYSTAL STRUCTURE OF A HUMAN LOW MOLECULAR WEIGHT PHOSPHOTYROSYL PHOSPHATASE. IMPLICATIONS FOR SUBSTRATE SPECIFICITY
  5KQP	37%	Homo sapiens	Crystal structure of Apo-form LMW-PTP

• Links to additional information on ptpA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -3.496 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VSDPLHVTFVCTGNICRSPMAEKMFAQQLRHRGLGDAVRVTSAGTGNWHVGSCADERAAGVLRAHGYPTDHRAAQVGTEHLAADLLVALDRNHARLLRQL
GVEAARVRMLRSFDPRSGTHALDVEDPYYGDHSDFEEVFAVIESALPGLHDWVDERLARNGPS

Additional Information