TB Genome Annotation Portal

Rv2220 (glnA1)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: glutamine synthetase 1
  • REFSEQ: glutamine synthetase GLNA1 (glutamine synthase) (GS-I)
  • PATRIC: Glutamine synthetase type I (EC 6.3.1.2)
  • TUBERCULIST: Glutamine synthetase GlnA1 (glutamine synthase) (GS-I)
  • NCBI: Glutamine synthetase GlnA1 (glutamine synthase) (GS-I)
  • updated information (H37Rv4):
    • gene name: glnA1
    • function:
    • reference:
• Type: Not Target
• Start: 2487615
• End: 2489051
• Operon:


• Trans-membrane region:
  
• Role: I.D.1 - Glutamate family
• GO terms:
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0044044 - interaction with host via substance in symbiont surface (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0035375 - zymogen binding (Uniprot)
  • GO:0020012 - evasion or tolerance of host immune response (Uniprot)
  • GO:0016874 - ligase activity (Uniprot)
  • GO:0010756 - positive regulation of plasminogen activation (Uniprot)
  • GO:0009405 - pathogenesis (Uniprot)
  • GO:0009399 - nitrogen fixation (Uniprot)
  • GO:0006807 - nitrogen compound metabolic process (Uniprot)
  • GO:0006542 - glutamine biosynthetic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005576 - extracellular region (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0004356 - glutamate-ammonia ligase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
  • GO:0001968 - fibronectin binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • 3.4 GLU[c] + 3.4 GLN[c] -> P_L_GLX[c]
  • ATP[c] + GLU[c] + NH3[c] -> ADP[c] + PI[c] + GLN[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 4acf, 2wgs, 2bvc, 2whi, 3zxr, 3zxv
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4ACF	100%	MYCOBACTERIUM TUBERCULOSIS	CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH IMIDAZOPYRIDINE INHIBITOR ((4-(6-BROMO-3-(BUTYLAMINO)IMIDAZO(1,2-A)PYRIDIN-2-YL)PHENOXY) ACETIC ACID) AND L-METHIONINE-S-SULFOXIMINE PHOSPHATE.
  3ZXV	100%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with tri-substituted imidazole inhibitor (4-(2-tert-butyl- 4-(6-methoxynaphthalen-2-yl)-1H-imidazol-5-yl)pyridin-2-amine) and L- methionine-S-sulfoximine phosphate
  3ZXR	100%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with tri-substituted imidazole inhibitor (3-(2-tert-butyl- 5-(pyridin-4-yl)-1H-imidazol-4-yl)quinoline) and L-methionine-S- sulfoximine phosphate.
  2WHI	100%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with a purine analogue inhibitor and L-methionine-S- sulfoximine phosphate.
  2WGS	100%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium Tuberculosis Glutamine Synthetase in complex with a purine analogue inhibitor.
  2BVC	100%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium tuberculosis glutamine synthetase in complex with a transition state mimic
  1HTQ	100%	Mycobacterium tuberculosis	Multicopy crystallographic structure of a relaxed glutamine synthetase from Mycobacterium tuberculosis
  1HTO	100%	Mycobacterium tuberculosis	CRYSTALLOGRAPHIC STRUCTURE OF A RELAXED GLUTAMINE SYNTHETASE FROM MYCOBACTERIUM TUBERCULOSIS
  4XYC	99%	Mycobacterium tuberculosis	NANOMOLAR INHIBITORS OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE 1: SYNTHESIS, BIOLOGICAL EVALUATION AND X-RAY CRYSTALLOGRAPHIC STUDIES
  4S17	62%	Bifidobacterium adolescentis	The crystal structure of glutamine synthetase from Bifidobacterium adolescentis ATCC 15703

• Links to additional information on glnA1:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -7.393 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VTEKTPDDVFKLAKDEKVEYVDVRFCDLPGIMQHFTIPASAFDKSVFDDGLAFDGSSIRGFQSIHESDMLLLPDPETARIDPFRAAKTLNINFFVHDPFT
LEPYSRDPRNIARKAENYLISTGIADTAYFGAEAEFYIFDSVSFDSRANGSFYEVDAISGWWNTGAATEADGSPNRGYKVRHKGGYFPVAPNDQYVDLRD
KMLTNLINSGFILEKGHHEVGSGGQAEINYQFNSLLHAADDMQLYKYIIKNTAWQNGKTVTFMPKPLFGDNGSGMHCHQSLWKDGAPLMYDETGYAGLSD
TARHYIGGLLHHAPSLLAFTNPTVNSYKRLVPGYEAPINLVYSQRNRSACVRIPITGSNPKAKRLEFRSPDSSGNPYLAFSAMLMAGLDGIKNKIEPQAP
VDKDLYELPPEEAASIPQTPTQLSDVIDRLEADHEYLTEGGVFTNDLIETWISFKRENEIEPVNIRPHPYEFALYYDV

Additional Information