TB Genome Annotation Portal

Rv2210c (ilvE)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: branched-chain-amino-acid aminotransferase
  • REFSEQ: branched-chain amino acid aminotransferase
  • PATRIC: Branched-chain amino acid aminotransferase (EC 2.6.1.42)
  • TUBERCULIST: Branched-chain amino acid transaminase IlvE
  • NCBI: Branched-chain amino acid transaminase IlvE
  • updated information (H37Rv4):
    • gene name: ilvE
    • function:
    • reference:
• Type: Not Target
• Start: 2474864
• End: 2475970
• Operon:


• Trans-membrane region:
  
• Role: I.D.7 - Branched amino acid family
• GO terms:
  • GO:0071267 - L-methionine salvage (Uniprot)
  • GO:0052656 - L-isoleucine transaminase activity (Uniprot)
  • GO:0052655 - L-valine transaminase activity (Uniprot)
  • GO:0052654 - L-leucine transaminase activity (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0030170 - pyridoxal phosphate binding (Uniprot)
  • GO:0018272 - protein-pyridoxal-5-phosphate linkage via peptidyl-N6-pyridoxal phosphate-L-lysine (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0009099 - valine biosynthetic process (Uniprot)
  • GO:0009098 - leucine biosynthetic process (Uniprot)
  • GO:0009097 - isoleucine biosynthetic process (Uniprot)
  • GO:0009082 - branched-chain amino acid biosynthetic process (Uniprot)
  • GO:0009081 - branched-chain amino acid metabolic process (Uniprot)
  • GO:0008652 - cellular amino acid biosynthetic process (Uniprot)
  • GO:0008483 - transaminase activity (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0004084 - branched-chain-amino-acid transaminase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • AKG[c] + ILE[c] <=> GLU[c] + OMVAL[c]
  • GLU[c] + OMVAL[c] <=> AKG[c] + VAL[c]
  • GLU[c] + OXOMETHYLPENTANOATE[c] <=> AKG[c] + ILE[c]
  • GLU[c] + MOP[c] <=> AKG[c] + LEU[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 3ht5
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5U3F	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Structure of Mycobacterium tuberculosis IlvE, a branched-chain amino acid transaminase, in complex with D-cycloserine derivative
  3HT5	100%	Mycobacterium tuberculosis	Crystal Structure of IlvE a Branched Chain Amino Acid Transaminase from Mycobacterium tuberculosis
  3JZ6	84%	Mycobacterium smegmatis	Crystal structure of Mycobacterium smegmatis Branched Chain Aminotransferase in complex with pyridoxal-5'-phosphate at 1.9 angstrom.
  3DTG	84%	Mycobacterium smegmatis	Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
  3DTF	84%	Mycobacterium smegmatis	Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
  3UZO	42%	Deinococcus radiodurans	Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis
  3UZB	42%	Deinococcus radiodurans	Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis
  3UYY	42%	Deinococcus radiodurans	Crystal Structures of Branched-Chain Aminotransferase from Deinococcus radiodurans Complexes with alpha-Ketoisocaproate and L-Glutamate Suggest Its Radio-Resistance for Catalysis
  4DQN	40%	Streptococcus mutans	Crystal structure of the branched-chain aminotransferase from Streptococcus mutans
  5I60	39%	Homo sapiens	X-RAY CRYSTAL STRUCTURE AT 2.12A RESOLUTION OF HUMAN MITOCHONDRIAL BRANCHED CHAIN AMINOTRANSFERASE (BCATM) COMPLEXED WITH A BIARL AMIDE COMPOUND AND AN INTERNAL ALDIMINE LINKED PLP COFACTOR.

• Links to additional information on ilvE:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -8.438 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MTSGSLQFTVLRAVNPATDAQRESMLREPGFGKYHTDHMVSIDYAEGRGWHNARVIPYGPIELDPSAIVLHYAQEVFEGLKAYRWADGSIVSFRADANAA
RLRSSARRLAIPELPDAVFIESLRQLIAVDKAWVPGAGGEEALYLRPFIFATEPGLGVRPATQYRYLLIASPAGAYFKGGIAPVSVWVSTEYVRACPGGT
GAAKFGGNYAASLLAQAEAAENGCDQVVWLDAVERRYIEEMGGMNIFFVLGSGGSARLVTPELSGSLLPGITRDSLLQLAIDAGFAVEERRIDIDEWQKK
AAAGEITEVFACGTAAVITPVARVRHGASEFRIADGQPGEVTMALRDTLTGIQRGTFADTHGWMARLG

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	1.15 (0.12)	1.07 (0.37)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link