TB Genome Annotation Portal

Rv2195 (qcrA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: ubiquinol-cytochrome c reductase iron-sulfur subunit
  • REFSEQ: Rieske iron-sulfur protein QcrA
  • PATRIC: Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2)
  • TUBERCULIST: Probable rieske iron-sulfur protein QcrA
  • NCBI: Probable rieske iron-sulfur protein QcrA
  • updated information (H37Rv4):
    • gene name: qcrA
    • function:
    • reference:
• Type: Not Target
• Start: 2458392
• End: 2459681
• Operon:


• Trans-membrane region:
  
• Role: I.B.6.a - aerobic
• GO terms:
  • GO:0070469 - respiratory chain (Uniprot)
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0051537 - 2 iron, 2 sulfur cluster binding (Uniprot)
  • GO:0051536 - iron-sulfur cluster binding (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016679 - oxidoreductase activity, acting on diphenols and related substances as donors (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0005887 - integral component of plasma membrane (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • 0.5 O2[c] + 2 HEME_FE2[c] -> 6 H[c] + 2 HEME_FE3[c]
  • MKH2[c] + 2 HEME_FE3[c] <=> MK[c] + 2 HEME_FE2[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6HWH	79%	Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)	Structure of a functional obligate respiratory supercomplex from Mycobacterium smegmatis
  6ADQ	79%	Mycobacterium smegmatis MC2 51	Respiratory Complex CIII2CIV2SOD2 from Mycobacterium smegmatis
  3AZC	37%	Thermosynechococcus elongatus	Crystal structure of the soluble part of cytochrome b6f complex iron-sulfur subunit from Thermosynechococcus elongatus BP-1
  3FOU	37%	Thermus thermophilus	Low pH structure of the Rieske protein from Thermus thermophilus at 2.1 A
  1NYK	37%	Thermus thermophilus	Crystal Structure of the Rieske protein from Thermus thermophilus
  1G8K	35%	Alcaligenes faecalis	CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS
  1G8J	35%	Alcaligenes faecalis	CRYSTAL STRUCTURE ANALYSIS OF ARSENITE OXIDASE FROM ALCALIGENES FAECALIS

• Links to additional information on qcrA:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -8.875 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MSRADDDAVGVPPTCGGRSDEEERRIVPGPNPQDGAKDGAKATAVPREPDEAALAAMSNQELLALGGKLDGVRIAYKEPRWPVEGTKAEKRAERSVAVWL
LLGGVFGLALLLIFLFWPWEFKAADGESDFIYSLTTPLYGLTFGLSILSIAIGAVLYQKRFIPEEISIQERHDGASREIDRKTVVANLTDAFEGSTIRRR
KLIGLSFGVGMGAFGLGTLVAFAGGLIKNPWKPVVPTAEGKKAVLWTSGWTPRYQGETIYLARATGTEDGPPFIKMRPEDMDAGGMETVFPWRESDGDGT
TVESHHKLQEIAMGIRNPVMLIRIKPSDLGRVVKRKGQESFNFGEFFAFTKVCSHLGCPSSLYEQQSYRILCPCHQSQFDALHFAKPIFGPAARALAQLP
ITIDTDGYLVANGDFVEPVGPAFWERTTT

Additional Information