TB Genome Annotation Portal

Rv2121c (hisG)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: ATP phosphoribosyltransferase
  • REFSEQ: ATP phosphoribosyltransferase
  • PATRIC: ATP phosphoribosyltransferase (EC 2.4.2.17)
  • TUBERCULIST: ATP phosphoribosyltransferase HisG
  • NCBI: ATP phosphoribosyltransferase HisG
  • updated information (H37Rv4):
    • gene name: hisG
    • function:
    • reference:
• Type: Not Target
• Start: 2379806
• End: 2380660
• Operon:


• Trans-membrane region:
  
• Role: I.D.5 - Histidine
• GO terms:
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016757 - transferase activity, transferring glycosyl groups (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016208 - AMP binding (Uniprot)
  • GO:0008652 - cellular amino acid biosynthetic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0005515 - protein binding (Uniprot)
  • GO:0003879 - ATP phosphoribosyltransferase activity (Uniprot)
  • GO:0000287 - magnesium ion binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
  • GO:0000105 - histidine biosynthetic process (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ATP[c] + PRPP[c] -> PPI[c] + PRBATP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 1nh7, 1nh8
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  5U99	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Transition state analysis of adenosine triphosphate phosphoribosyltransferase
  5LHU	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric inhibitor L-Histidine
  5LHT	100%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	ATP Phosphoribosyltransferase from Mycobacterium tuberculosis in complex with the allosteric activator 3-(2-Thienyl)-L-alanine
  1NH8	100%	Mycobacterium tuberculosis H37Rv	ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS IN COMPLEX WITH AMP AND HISTIDINE
  1NH7	100%	Mycobacterium tuberculosis H37Rv	ATP PHOSPHORIBOSYLTRANSFERASE (ATP-PRTASE) FROM MYCOBACTERIUM TUBERCULOSIS
  1VE4	35%	Thermus thermophilus	ATP-Phosphoribosyltransferase(hisG) from Thermus thermophilus HB8

• Links to additional information on hisG:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -9.384 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • BioCyc
  • Systems Biology
  • Mycobrowser
  • PATRIC

Amino Acid Sequence

MLRVAVPNKGALSEPATEILAEAGYRRRTDSKDLTVIDPVNNVEFFFLRPKDIAIYVGSGELDFGITGRDLVCDSGAQVRERLALGFGSSSFRYAAPAGR
NWTTADLAGMRIATAYPNLVRKDLATKGIEATVIRLDGAVEISVQLGVADAIADVVGSGRTLSQHDLVAFGEPLCDSEAVLIERAGTDGQDQTEARDQLV
ARVQGVVFGQQYLMLDYDCPRSALKKATAITPGLESPTIAPLADPDWVAIRALVPRRDVNGIMDELAAIGAKAILASDIRFCRF

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	1.23 (0.12)	1.48 (0.59)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link