TB Genome Annotation Portal

Rv2051c (ppm1)

Amino Acid Sequence

VKLGAWVAAQLPTTRTAVRTRLTRLVVSIVAGLLLYASFPPRNCWWAAVVALALLAWVLTHRATTPVGGLGYGLLFGLVFYVSLLPWIGELVGPGPWLAL
ATTCALFPGIFGLFAVVVRLLPGWPIWFAVGWAAQEWLKSILPFGGFPWGSVAFGQAEGPLLPLVQLGGVALLSTGVALVGCGLTAIALEIEKWWRTGGQ
GDAPPAVVLPAACICLVLFAAIVVWPQVRHAGSGSGGEPTVTVAVVQGNVPRLGLDFNAQRRAVLDNHVEETLRLAADVHAGLAQQPQFVIWPENSSDID
PFVNPDAGQRISAAAEAIGAPILIGTLMDVPGRPRENPEWTNTAIVWNPGTGPADRHDKAIVQPFGEYLPMPWLFRHLSGYADRAGHFVPGNGTGVVRIA
GVPVGVATCWEVIFDRAPRKSILGGAQLLTVPSNNATFNKTMSEQQLAFAKVRAVEHDRYVVVAGTTGISAVIAPDGGELIRTDFFQPAYLDSQVRLKTR
LTPATRWGPILQWILVGAAAAVVLVAMRQNGWFPRPRRSEPKGENDDSDAPPGRSEASGPPALSESDDELIQPEQGGRHSSGFGRHRATSRSYMTTGQPA
PPAPGNRPSQRVLVIIPTFNERENLPVIHRRLTQACPAVHVLVVDDSSPDGTGQLADELAQADPGRTHVMHRTAKNGLGAAYLAGFAWGLSREYSVLVEM
DADGSHAPEQLQRLLDAVDAGADLAIGSRYVAGGTVRNWPWRRLVLSKTANTYSRLALGIGIHDITAGYRAYRREALEAIDLDGVDSKGYCFQIDLTWRT
VSNGFVVTEVPITFTERELGVSKMSGSNIREALVKVARWGIEGRLSRSDHARARPDIARPGAGGSRVSRADVTE
(Nucleotide sequence available on KEGG)

Additional Information




Analysis of Positive Selection in Clinical Isolates *new*

Moldova (2,057)global set (5,195)
under significant positive selection?NONO
omega peak height (95%CI lower bound)1.63 (0.35)1.24 (0.55)
codons under selection
omega plots
genetic variants*linklink
statistics at each codonlinklink
* example format for variants: "D27 (GAC): D27H (CAC,11)" means "Asp27 (native codon GAC) mutated to His (codon CAC) in 11 isolates"


ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across ~100 conditions

Rv2051c/ppm1, gene len: 2624 bp, num TA sites: 33
conditiondatasetcallmediummethodnotes
in-vitroDeJesus 2017 mBiodomain essential7H9HMMfully saturated, 14 TnSeq libraries combined
in-vitroSassetti 2003 Mol Micronon-essential 7H9TRASHessential if hybridization ratio<0.2
in-vivo (mice)Sassetti 2003 PNASnon-essential BL6 miceTRASHessential if hybridization ratio<0.4, min over 4 timepoints (1-8 weeks)
in-vitro (glycerol)Griffin 2011 PPathessentialM9 minimal+glycerolGumbel2 replicates; Padj<0.05
in-vitro (cholesterol)Griffin 2011 PPathessentialM9 minimal+cholesterolGumbel3 replicates; Padj<0.05
differentially essential in cholesterol Griffin 2011 PPathNO (LFC=0.18)cholesterol vs glycerolresampling-SRYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in cholesterol
in-vitroSmith 2022 eLifenon-essential7H9HMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
in-vivo (mice)Smith 2022 eLifenon-essentialBL6 miceHMM6 replicates (raw data in Subramaniam 2017, PMID 31752678)
differentially essential in miceSmith 2022 eLifeNO (LFC=-0.713)in-vivo vs in-vitroZINBYES if Padj<0.05, else not significant; LFC<0 means less insertions/more essential in mice
in-vitro (minimal)Minato 2019 mSysgrowth advantageminimal mediumHMM
in-vitro (YM rich medium)Minato 2019 mSysnon-essentialYM rich mediumHMM7H9 supplemented with ~20 metabolites (amino acids, cofactors)
differentially essential in YM rich mediumMinato 2019 mSysYES (LFC=-3.38)YM rich vs minimal mediumresampling

TnSeq Data No data currently available.
  • No TnSeq data currently available for this Target.
RNASeq Data No data currently available.
  • No RNA-Seq data currently available for this Target.
Metabolomic Profiles No data currently available.
  • No Metabolomic data currently available for this Target.
Proteomic Data No data currently available.
  • No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology
  • BioCyc

    Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

    NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

    Interactions based on ChIPSeq data

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

    Interactions based on ChIPSeq data (Minch et al. 2014)

    • Binds To:

      • No bindings to other targets were found.
    • Bound By:

      • No bindings to other targets were found.

    Interactions based on TFOE data (Rustad et al. 2014)

    • Upregulates:

      • Does not upregulate other genes.
    • Upregulated by:

      • Not upregulated by other genes.
    • Downregulates:

      • Does not downregulate other genes.
    • Downregulated by:



    TBCAP

    Tubculosis Community Annotation Project (
    Slayden et al., 2013)

    Rv2051c (ppm1)

    PropertyValueCreatorEvidencePMIDComment
    CitationPpm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. SS. Gurcha, AR. Baulard et al. Biochem. J. 2002njamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT2 gdp mannosyl phosphate transferase - ISSnjamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    SS. Gurcha, AR. Baulard et al. Ppm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. Biochem. J. 2002
    CitationMycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mol. Microbiol. 2004njamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT2 gdp mannosyl phosphate transferase - ISSnjamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol. Microbiol. 2004
    CitationPpm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. SS. Gurcha, AR. Baulard et al. Biochem. J. 2002njamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT2 gdp mannosyl phosphate transferase - IPInjamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    SS. Gurcha, AR. Baulard et al. Ppm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. Biochem. J. 2002
    CitationMycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mol. Microbiol. 2004njamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT2 gdp mannosyl phosphate transferase - IPInjamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol. Microbiol. 2004
    CitationPpm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. SS. Gurcha, AR. Baulard et al. Biochem. J. 2002njamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT1 gdp mannosyl phosphate transferase - ISSnjamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    SS. Gurcha, AR. Baulard et al. Ppm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. Biochem. J. 2002
    CitationMycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mol. Microbiol. 2004njamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT1 gdp mannosyl phosphate transferase - ISSnjamshidiISS11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol. Microbiol. 2004
    CitationPpm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. SS. Gurcha, AR. Baulard et al. Biochem. J. 2002njamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT1 gdp mannosyl phosphate transferase - IPInjamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    SS. Gurcha, AR. Baulard et al. Ppm1, a novel polyprenol monophosphomannose synthase from Mycobacterium tuberculosis. Biochem. J. 2002
    CitationMycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mol. Microbiol. 2004njamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    TermTBRXN:GMT1 gdp mannosyl phosphate transferase - IPInjamshidiIPI11931640|15228522known to occur physiologically (see Tuberculist for example - also demonstrated biochemically see PMID: 11931640, 15228522) - specific biochemical mechanism has not been elucidated.
    authors,V. Briken,SA. Porcelli,GS. Besra,L. Kremer Mycobacterial lipoarabinomannan and related lipoglycans: from biogenesis to modulation of the immune response. Mol. Microbiol. 2004

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