TB Genome Annotation Portal

Rv1992c (ctpG)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: heavy metal translocating P-type ATPase
  • REFSEQ: metal cation transporter P-type ATPase G CtpG
  • PATRIC: Lead cadmium zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)
  • TUBERCULIST: Probable metal cation transporter P-type ATPase G CtpG
  • NCBI: Probable metal cation transporter P-type ATPase G CtpG
  • updated information (H37Rv4):
    • gene name: ctpG
    • function:
    • reference:
• Coordinates in H37Rv: 2234991 - 2237306
• Gene length: 2316 bp (with stop codon), 771 aa (without stop codon)
• Operon:


• Trans-membrane region:
  
• Role: III.A.2 - Cations
• GO terms:
  • GO:0099132 - ATP hydrolysis coupled cation transmembrane transport (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0046688 - response to copper ion (Uniprot)
  • GO:0019829 - cation-transporting ATPase activity (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0016021 - integral component of membrane (Uniprot)
  • GO:0016020 - membrane (Uniprot)
  • GO:0006812 - cation transport (Uniprot)
  • GO:0005887 - integral component of plasma membrane (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005524 - ATP binding (Uniprot)
  • GO:0000166 - nucleotide binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Latest Homologs in PDB
• Top 10 Homologs in PDB (as of Apr 2026):
  

  PDB	aa ident	species	PDB title
  2VOY	42%	ARCHAEOGLOBUS FULGIDUS	CryoEM model of CopA, the copper transporting ATPase from Archaeoglobus fulgidus
  2HC8	42%	Archaeoglobus fulgidus	Structure of the A. fulgidus CopA A-domain
  7QC0	40%	Sulfitobacter sp. (strain NAS-14.1)	Crystal structure of Cadmium translocating P-type ATPase
  7QBZ	40%	Sulfitobacter sp. (strain NAS-14.1)	Crystal structure Cadmium translocating P-type ATPase
  3SKY	40%	Archaeoglobus fulgidus	2.1A crystal structure of the phosphate bound ATP binding domain of Archaeoglobus fulgidus COPB
  3SKX	40%	Archaeoglobus fulgidus	Crystal structure of the ATP binding domain of Archaeoglobus fulgidus COPB
  4UMW	39%	SHIGELLA SONNEI	CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN E2.PI STATE
  4UMV	39%	SHIGELLA SONNEI	CRYSTAL STRUCTURE OF A ZINC-TRANSPORTING PIB-TYPE ATPASE IN THE E2P STATE
  3A1D	38%	Archaeoglobus fulgidus	Crystal structure of the P- and N-domains of CopA, a copper-transporting P-type ATPase, bound with ADP-Mg
  3A1C	38%	Archaeoglobus fulgidus	crystal structure of the P- and N-domains of CopA, a copper-transporting P-type ATPase, bound with AMPPCP-Mg

• Links to additional information on ctpG:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.087 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VTTVVDAEVQLTVVSDAAGRMRVQATGFQFDAGRAVAIEDTVGKVAGVQAVHAYPRTASIVIWYSRAICDTAAILSAIIDAETVPAAAVPAYASRSASNR
KAGVVQKIIDWSTRTLSGVRRDVAAQPSGETSDACCDGEDNEDREPEQLWQVAKLRRAAFSGVLLTASLVAAWAYPLWPVVLGLKALALAVGASTFVPSS
LKRLAEGRVGVGTLMTIAALGAVALGELGEAATLAFLFSISEGLEEYATARTRRGLRALLSLVPDQATVLREGTETIVASTELHVGDQMIVKPGERLATD
GIIRAGRTALDVSAITGESVPVEVGPGDEVFAGSINGLGVLQVGVTATAANNSLARIVHIVEAEQVRKGASQRLADCIARPLVPSIMIAAALIAGTGSVL
GNPLVWIERALVVLVAAAPCALAIAVPVTVVASIGAASRLGVLIKGGAALETLGTIRAVALDKTGTLTANRPVVIDVATTNGATREEVLAVAAALEARSE
HPLAVAVLAATQATTAASDVQAVPGAGLIGRLDGRVVRLGRPGWLDAAELADHVACMQQAGATAVLVERDQQLLGAIAVRDELRPEAAEVVAGLRTGGYQ
VTMLTGDNHATAAALAAQAGIEQVHAELRPEDKAHLVAQLRARQPTAMVGDGVNDAPALAAADLGIAMGAMGTDVAIETADVALMGQDLRHLPQALDHAR
RSRQIMVQNVGLSLSIITVLMPLALFGILGLAAVVLVHEFTEVIVIANGVRAGRIKPLAGPPKTPDRTIPG

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• data from Culviner et al (2025) (55,259 Mtb clinical isolates)
  • overall pN/pS for Rv1992c: 0.94957389
  • lineage-specific pN/pS in L1: 1.326720938
  • lineage-specific pN/pS in L2: 1.053308853
  • lineage-specific pN/pS in L3: 0.986076373
  • lineage-specific pN/pS in L4: 0.795833356

• Analysis of dN/dS (omega) in a global collection of 10k Mtb clinical isolates using GenomegaMap (Window model)
• clinical isolates collection: global set of 10,626 Mtb genomes
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	global set of 10,626 Mtb clinical isolates
under significant positive selection?	NO
omega peak height (95%CI lower bound)	1.84 (0.92)
codons under selection
omega plots
genetic variants*	link