TB Genome Annotation Portal

Rv1850 (ureC)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: urease subunit alpha
  • REFSEQ: urease subunit alpha
  • PATRIC: Urease alpha subunit (EC 3.5.1.5)
  • TUBERCULIST: Urease alpha subunit UreC (urea amidohydrolase)
  • NCBI: Urease alpha subunit UreC (urea amidohydrolase)
  • updated information (H37Rv4):
    • gene name: ureC
    • function:
    • reference:
• Type: Not Target
• Start: 2097961
• End: 2099694
• Operon:


• Trans-membrane region:
  
• Role: I.A.2 - Amino acids and amines
• GO terms:
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0043419 - urea catabolic process (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016810 - hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0016151 - nickel cation binding (Uniprot)
  • GO:0009039 - urease activity (Uniprot)
  • GO:0006807 - nitrogen compound metabolic process (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4EPE	60%	Enterobacter aerogenes	Final Urease Structure for Radiation Damage Experiment at 300 K
  4EPD	60%	Enterobacter aerogenes	Initial Urease Structure for Radiation Damage Experiment at 300 K
  4EPB	60%	Enterobacter aerogenes	Final Urease Structure for Radiation Damage Experiment at 100 K
  4EP8	60%	Enterobacter aerogenes	Initial Urease Structure for Radiation Damage Experiment at 100 K
  1EF2	60%	Klebsiella aerogenes	CRYSTAL STRUCTURE OF MANGANESE-SUBSTITUTED KLEBSIELLA AEROGENES UREASE
  2KAU	59%	Klebsiella aerogenes	THE CRYSTAL STRUCTURE OF UREASE FROM KLEBSIELLA AEROGENES AT 2.2 ANGSTROMS RESOLUTION
  1KRA	59%	Klebsiella aerogenes	CRYSTAL STRUCTURE OF KLEBSIELLA AEROGENES UREASE, ITS APOENZYME AND TWO ACTIVE SITE MUTANTS
  1FWJ	59%	Klebsiella aerogenes	KLEBSIELLA AEROGENES UREASE, NATIVE
  1EJX	59%	Klebsiella aerogenes	CRYSTAL STRUCTURE OF WILD-TYPE KLEBSIELLA AEROGENES UREASE AT 100K
  1EJW	59%	Klebsiella aerogenes	CRYSTAL STRUCTURE OF WILD-TYPE KLEBSIELLA AEROGENES UREASE AT 298K

• Links to additional information on ureC:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.391 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MARLSRERYAQLYGPTTGDRIRLADTNLLVEVTEDRCGGPGLAGDEAVFGGGKVLRESMGQGRASRADGAPDTVITGAVIIDYWGIIKADIGIRDGRIVG
IGKAGNPDIMTGVHRDLVVGPSTEIISGNRRIVTAGTVDCHVHLICPQIIVEALAAGTTTIIGGGTGPAEGTKATTVTPGEWHLARMLESLDGWPVNFAL
LGKGNTVNPDALWEQLRGGASGFKLHEDWGSTPAAIDTCLAVADVAGVQVALHSDTLNETGFVEDTIGAIAGRSIHAYHTEGAGGGHAPDIITVAAQPNV
LPSSTNPTRPHTVNTLDEHLDMLMVCHHLNPRIPEDLAFAESRIRPSTIAAEDVLHDMGAISMIGSDSQAMGRVGEVVLRTWQTAHVMKARRGALEGDPS
GSQAADNNRVRRYIAKYTICPAIAHGMDHLIGSVEVGKLADLVLWEPAFFGVRPHVVLKGGAIAWAAMGDANASIPTPQPVLPRPMFGAAAATAAATSVH
FVAPQSIDARLADRLAVNRGLAPVADVRAVGKTDLPLNDALPSIEVDPDTFTVRIDGQVWQPQPAAELPMTQRYFLF

Additional Information