TB Genome Annotation Portal

Rv1659 (argH)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: argininosuccinate lyase
  • REFSEQ: argininosuccinate lyase
  • PATRIC: Argininosuccinate lyase (EC 4.3.2.1)
  • TUBERCULIST: Probable argininosuccinate lyase ArgH
  • NCBI: Probable argininosuccinate lyase ArgH
  • updated information (H37Rv4):
    • gene name: argH
    • function:
    • reference:
• Type: Not Target
• Start: 1872639
• End: 1874051
• Operon:


• Trans-membrane region:
  
• Role: I.D.1 - Glutamate family
• GO terms:
  • GO:0042450 - arginine biosynthetic process via ornithine (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016829 - lyase activity (Uniprot)
  • GO:0008652 - cellular amino acid biosynthetic process (Uniprot)
  • GO:0006526 - arginine biosynthetic process (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0004056 - argininosuccinate lyase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • ARGSUCC[c] -> FUM[c] + ARG[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6IGA	99%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis
  6IG5	99%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Crystal structure of argininosuccinate lyase from Mycobacterium tuberculosis
  6IEN	99%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Substrate/product bound Argininosuccinate lyase from Mycobacterium tuberculosis
  6IEM	99%	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	Argininosuccinate lyase from Mycobacterium tuberculosis
  2E9F	44%	Thermus thermophilus	Crystal Structure of T.th.HB8 Argininosuccinate lyase complexed with L-Arginine
  1AOS	43%	Homo sapiens	HUMAN ARGININOSUCCINATE LYASE
  1K62	42%	Homo sapiens	Crystal Structure of the Human Argininosuccinate Lyase Q286R Mutant
  1TJ7	41%	Escherichia coli	Structure determination and refinement at 2.44 A resolution of Argininosuccinate lyase from E. coli
  1DCN	41%	Anas platyrhynchos	INACTIVE MUTANT H162N OF DELTA 2 CRYSTALLIN WITH BOUND ARGININOSUCCINATE
  1U16	41%	Anas platyrhynchos	Crystal structure of a duck-delta-crystallin-1 double loop mutant (DLM) in complex with sulfate

• Links to additional information on argH:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -6.471 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VSTNEGSLWGGRFAGGPSDALAALSKSTHFDWVLAPYDLTASRAHTMVLFRAGLLTEEQRDGLLAGLDSLAQDVADGSFGPLVTDEDVHAALERGLIDRV
GPDLGGRLRAGRSRNDQVAALFRMWLRDAVRRVATGVLDVVGALAEQAAAHPSAIMPGKTHLQSAQPILLAHHLLAHAHPLLRDLDRIVDFDKRAAVSPY
GSGALAGSSLGLDPDAIAADLGFSAAADNSVDATAARDFAAEAAFVFAMIAVDLSRLAEDIIVWSSTEFGYVTLHDSWSTGSSIMPQKKNPDIAELARGK
SGRLIGNLAGLLATLKAQPLAYNRDLQEDKEPVFDSVAQLELLLPAMAGLVASLTFNVQRMAELAPAGYTLATDLAEWLVRQGVPFRSAHEAAGAAVRAA
EQRGVGLQELTDDELAAISPELTPQVREVLTIEGSVSARDCRGGTAPGRVAEQLNAIGEAAERLRRQLVR

Additional Information