TB Genome Annotation Portal

Rv1449c (tkt)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: transketolase
  • REFSEQ: transketolase
  • PATRIC: Transketolase (EC 2.2.1.1)
  • TUBERCULIST: Transketolase Tkt (TK)
  • NCBI: Transketolase Tkt (TK)
  • updated information (H37Rv4):
    • gene name: tkt
    • function:
    • reference:
• Type: Not Target
• Start: 1628097
• End: 1630199
• Operon:


• Trans-membrane region:
  
• Role: I.B.5 - Pentose phosphate pathway
• GO terms:
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0005576 - extracellular region (Uniprot)
  • GO:0004802 - transketolase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • R5P[c] + 0.001 THI[c] + X5P[c] -> G3P[c] + S7P[c]
  • G3P[c] + 0.001 THI[c] + S7P[c] -> R5P[c] + X5P[c]
  • 0.001 THI[c] + X5P[c] + E4P[c] -> F6P[c] + G3P[c]
  • F6P[c] + G3P[c] + 0.001 THI[c] -> X5P[c] + E4P[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 3rim
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  3RIM	99%	Mycobacterium tuberculosis	Crystal structure of mycobacterium tuberculosis Transketolase (Rv1449c)
  2E6K	50%	Thermus thermophilus	X-ray structure of Thermus thermopilus HB8 TT0505
  3M49	47%	Bacillus anthracis	Crystal Structure of Transketolase Complexed with Thiamine Diphosphate from Bacillus anthracis
  3HYL	47%	Bacillus anthracis	Crystal Structure of Transketolase from Bacillus anthracis
  5ND6	45%	Chlamydomonas reinhardtii	Crystal structure of apo transketolase from Chlamydomonas reinhardtii
  5ND5	45%	Chlamydomonas reinhardtii	Crystal structure of transketolase from Chlamydomonas reinhardtii in complex with TPP and Mg2+
  1ITZ	44%	Zea mays	Maize Transketolase in complex with TPP
  1TRK	44%	Saccharomyces cerevisiae	REFINED STRUCTURE OF TRANSKETOLASE FROM SACCHAROMYCES CEREVISIAE AT 2.0 ANGSTROMS RESOLUTION
  1TKC	44%	Saccharomyces cerevisiae	SPECIFICITY OF COENZYME BINDING IN THIAMIN DIPHOSPHATE DEPENDENT ENZYMES: CRYSTAL STRUCTURES OF YEAST TRANSKETOLASE IN COMPLEX WITH ANALOGS OF THIAMIN DIPHOSPHATE
  1TKB	44%	Saccharomyces cerevisiae	SPECIFICITY OF COENZYME BINDING IN THIAMIN DIPHOSPHATE DEPENDENT ENZYMES: CRYSTAL STRUCTURES OF YEAST TRANSKETOLASE IN COMPLEX WITH ANALOGS OF THIAMIN DIPHOSPHATE

• Links to additional information on tkt:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -6.886 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

VTTLEEISALTRPRHPDYWTEIDSAAVDTIRVLAADAVQKVGNGHPGTAMSLAPLAYTLFQRTMRHDPSDTHWLGRDRFVLSAGHSSLTLYIQLYLGGFG
LELSDIESLRTWGSKTPGHPEFRHTPGVEITTGPLGQGLASAVGMAMASRYERGLFDPDAEPGASPFDHYIYVIASDGDIEEGVTSEASSLAAVQQLGNL
IVFYDRNQISIEDDTNIALCEDTAARYRAYGWHVQEVEGGENVVGIEEAIANAQAVTDRPSFIALRTVIGYPAPNLMDTGKAHGAALGDDEVAAVKKIVG
FDPDKTFQVREDVLTHTRGLVARGKQAHERWQLEFDAWARREPERKALLDRLLAQKLPDGWDADLPHWEPGSKALATRAASGAVLSALGPKLPELWGGSA
DLAGSNNTTIKGADSFGPPSISTKEYTAHWYGRTLHFGVREHAMGAILSGIVLHGPTRAYGGTFLQFSDYMRPAVRLAALMDIDTIYVWTHDSIGLGEDG
PTHQPIEHLSALRAIPRLSVVRPADANETAYAWRTILARRNGSGPVGLILTRQGVPVLDGTDAEGVARGGYVLSDAGGLQPGEEPDVILIATGSEVQLAV
AAQTLLADNDILARVVSMPCLEWFEAQPYEYRDAVLPPTVSARVAVEAGVAQCWHQLVGDTGEIVSIEHYGESADHKTLFREYGFTAEAVAAAAERALDN

Additional Information