TB Genome Annotation Portal

Rv1447c (zwf2)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: glucose-6-phosphate 1-dehydrogenase
  • REFSEQ: glucose-6-phosphate 1-dehydrogenase
  • PATRIC: Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49)
  • TUBERCULIST: Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD)
  • NCBI: Probable glucose-6-phosphate 1-dehydrogenase Zwf2 (G6PD)
  • updated information (H37Rv4):
    • gene name: zwf2
    • function:
    • reference:
• Type: Not Target
• Start: 1625418
• End: 1626962
• Operon:


• Trans-membrane region:
  
• Role: I.B.5 - Pentose phosphate pathway
• GO terms:
  • GO:0055114 - oxidation-reduction process (Uniprot)
  • GO:0050661 - NADP binding (Uniprot)
  • GO:0016491 - oxidoreductase activity (Uniprot)
  • GO:0006098 - pentose-phosphate shunt (Uniprot)
  • GO:0006006 - glucose metabolic process (Uniprot)
  • GO:0005975 - carbohydrate metabolic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0004345 - glucose-6-phosphate dehydrogenase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • NADP[c] + G6P[c] + 0.001 F420[c] -> NADPH[c] + D6PGL[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6JYU	37%	Homo sapiens	Crystal structure of Human G6PD Canton
  6E08	37%	Homo sapiens	Crystal structure of G6PD in complex with structural NADP
  6E07	37%	Homo sapiens	Crystal structure of Canton G6PD in complex with structural NADP
  5UKW	37%	Homo sapiens	Crystal structure of human Glucose 6-phosphate Dehydrogenase mutant (A277C) complexed with G6P
  2BHL	37%	HOMO SAPIENS	X-RAY STRUCTURE OF HUMAN GLUCOSE-6-PHOSPHATE DEHYDROGENASE (DELETION VARIANT) COMPLEXED WITH GLUCOSE-6-PHOSPHATE
  2BH9	37%	HOMO SAPIENS	X-RAY STRUCTURE OF A DELETION VARIANT OF HUMAN GLUCOSE 6-PHOSPHATE DEHYDROGENASE COMPLEXED WITH STRUCTURAL AND COENZYME NADP
  1QKI	37%	HOMO SAPIENS	X-RAY STRUCTURE OF HUMAN GLUCOSE 6-PHOSPHATE DEHYDROGENASE (VARIANT CANTON R459L) COMPLEXED WITH STRUCTURAL NADP+
  5AQ1	37%	TRYPANOSOMA CRUZI	Trypanosoma cruzi Glucose-6-phosphate Dehydrogenase in complex with G6P and NADPH
  6D24	36%	Trypanosoma cruzi	Trypanosoma cruzi Glucose-6-P Dehydrogenase in complex with G6P
  6D24	36%	Trypanosoma cruzi	Trypanosoma cruzi Glucose-6-P Dehydrogenase in complex with G6P

• Links to additional information on zwf2:
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.965 (from Jeremy Rock's lab)
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MKPAHAAASWRNPLRDKRDKRLPRIAGPCGMVIFGVTGDLARKKVMPAVYDLANRGLLPPTFSLVGFARRDWSTQDFGQVVYNAVQEHCRTPFRQQNWDR
LAEGFRFVPGTFDDDDAFAQLAETLEKLDAERGTGGNHAFYLAIPPKSFPVVCEQLHKSGLARPQGDRWSRVVIEKPFGHDLASARELNKAVNAVFPEEA
VFRIDHYLGKETVQNILALRFANQLFDPIWNAHYVDHVQITMAEDIGLGGRAGYYDGIGAARDVIQNHLMQLLALTAMEEPVSFHPAALQAEKIKVLSAT
RLAEPLDQTTSRGQYAAGWQGGEKVVGLLDEEGFAEDSTTETFAAITLEVDTRRWAGVPFYLRTGKRLGRRVTEIALVFRRAPHLPFDATMTDELGTNAM
VIRVQPDEGVTLRFGSKVPGTAMEVRDVNMDFSYGSAFAEDSPEAYERLILDVLLGEPSLFPVNAEVELAWEILDPALEHWAAHGTPDAYEAGTWGPESS
LEMLRRTGREWRRP

Additional Information

Analysis of Positive Selection in Clinical Isolates *new*

• Analysis of dN/dS (omega) in two collections of Mtb clinical isolates using GenomegaMap (Window model) (see description of methods)
  • Moldova: 2,057 clinical isolates
  • global set: 5,195 clinical isolates from 15 other countries
• In the omega plots, the black line shows the mean estimate of omega (dN/dS) at each codon, and the blue lines are the bounds for the 95% credible interval (95%CI, from MCMC sampling).
• A gene is under significant positive selection if the lower-bound of the 95%CI of omega (lower blue line) exceeds 1.0 at any codon.

	Moldova (2,057)	global set (5,195)
under significant positive selection?	NO	NO
omega peak height (95%CI lower bound)	2.49 (0.62)	1.74 (0.61)
codons under selection
omega plots
genetic variants*	link	link
statistics at each codon	link	link