Rv1405c (-)

Current annotations:
- TBCAP: (community-based annotations - see table at bottom of page)
- TBDB: methyltransferase
- REFSEQ: putative methyltransferase
- PATRIC: Methyltransferase
- TUBERCULIST: Putative methyltransferase
- NCBI: Putative methyltransferase
- updated information (H37Rv4):
  - gene name: -
  - function:
  - reference:
Type: Conditional
Start: 1581145
End: 1581969
Operon:

Trans-membrane region:
Role: IV.H - Miscellaneous transferases
GO terms:
- GO:0032259 - methylation (Uniprot)
- GO:0008168 - methyltransferase activity (Uniprot)
- GO:0008152 - metabolic process (Uniprot)
Reaction(s) (based on iSM810 metabolic model):
Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
Orthologs among selected mycobacteria
Protein structure:
Search for Homologs in PDB
Top 10 Homologs in PDB (as of Nov 2020): (none with >35% aa id)
Links to additional information on Rv1405c:
- TBDB (updated)
- Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
- UniProt
- AlphaFold model
- Vulnerability = 0.648 (from Jeremy Rock's lab)
- KEGG - nucleotide and amino acid sequences of gene
- Mycobrowser
- PATRIC
- BioCyc
- Systems Biology

Amino Acid Sequence

MTIDTPAREDQTLAATHRAMWALGDYALMAEEVMAPLGPILVAAAGIGPGVRVLDVAAGSGNISLPAAKTGATVISTDLTPELLQRSQARAAQQGLTLQY
QEANAQALPFADDEFDTVISAIGVMFAPDHQAAADELVRVCRPGGTIGVISWTCEGFFGRMLATIRPYRPSVSADLPPSALWGREAYVTGLLGDGVTGLK
TARGLLEVKRFDTAQAVHDYFKNNYGPTIEAYAHIGDNAVLAAELDRQLVELAAQYLSDGVMEWEYLLLTAEKR

(Nucleotide sequence available on KEGG)

Additional Information

ESSENTIALITY

MtbTnDB - interactive tool for exploring a database of published TnSeq datasets for Mtb

TnSeqCorr - genes with correlated TnSeq profiles across >100 conditions *new*

Classification	Condition	Strain	Method	Reference	Notes
Non-Essential	Sodium Oleate	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 12 sites
Non-Essential	Lignoceric Acid	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 12 sites
Non-Essential	Phosphatidylcholine	H37RvMA	Gumbel	Subhalaxmi Nambi	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 12 sites
Non-Essential	minimal media + 0.1% glycerol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 12 sites
Non-Essential	minimal media + 0.01% cholesterol	H37RvMA	Gumbel	Griffin et al. (2011)	Probability of Essentiality: 0.000000; 1 non-insertions in a row out of 12 sites
Non-Essential	7H10-glycerol	H37RvMA	TraSH	Sassetti et al. (2003a)
Non-Essential	C57BL/6J mice (8 weeks)	H37RvMA	TraSH	Sassetti et al. (2003b)	Hybridization Ratio: 2.09
Non-Essential	7H09/7H10 + rich media	H37RvMA	MotifHMM	DeJesus et al. (2017)	Fully saturated (14 reps).

TnSeq Data No data currently available.

No TnSeq data currently available for this Target.

RNASeq Data No data currently available.

No RNA-Seq data currently available for this Target.

Metabolomic Profiles No data currently available.

No Metabolomic data currently available for this Target.

Proteomic Data No data currently available.

No Proteomic data currently available for this Target.

Regulatory Relationships from Systems Biology

BioCyc

Gene interactions based on ChIPSeq and Transcription Factor Over-Expression (TFOE) (Systems Biology)

NOTE: Green edges represent the connected genes being classified as differentially essential as a result of the middle gene being knocked out. These interactions are inferred based on RNASeq.

Interactions based on ChIPSeq data

Binds To:
- No bindings to other targets were found.
Bound By:

Interactions based on ChIPSeq data (Minch et al. 2014)

Binds To:
- No bindings to other targets were found.
Bound By:
- Rv0081 (-) (Direct binding)
- Rv0967 (csoR) (Direct binding)
- Rv1353c (-) (Direct binding)
- Rv1404 (-) (Direct binding)
- Rv2887 (-) (Direct binding)
- Rv3133c (devR) (Direct binding)

Interactions based on TFOE data (Rustad et al. 2014)

Upregulates:
- Does not upregulate other genes.
Upregulated by:
Downregulates:
- Does not downregulate other genes.
Downregulated by:
- Not downregulated by other genes.

TBCAP

Tubculosis Community Annotation Project (

Slayden et al., 2013

Rv1405c (-)

Property	Value	Creator	Evidence	PMID	Comment
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007
Citation	Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Microbiology (Reading, Engl.) 2007	ashwinigbhat	IMP	17906132	None
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology (Reading, Engl.) 2007
Citation	Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. P. Golby, J. Nunez et al. Microbiology (Reading, Engl.) 2008	ashwinigbhat	IMP	18375799	None
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008
Interaction	Regulatory Rv1404	ashwinigbhat	IEP		qRT-PCR P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008
Citation	Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. authors,KH. Rohde,RB. Abramovitch,DG. Russell Cell Host Microbe 2007	priti.priety	IMP	18005756	None
Interaction	Regulatory Rv1403c	priti.priety	IMP		authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007
Citation	Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Microbiology (Reading, Engl.) 2007	priti.priety	IMP	17906132	None
Interaction	Regulatory Rv1403c	priti.priety	IMP		authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology (Reading, Engl.) 2007
Citation	Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. P. Golby, J. Nunez et al. Microbiology (Reading, Engl.) 2008	priti.priety	IMP	18375799	None
Interaction	Regulatory Rv1403c	priti.priety	IMP		P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008
Citation	Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. authors,KH. Rohde,RB. Abramovitch,DG. Russell Cell Host Microbe 2007	ashwinigbhat	IMP	18005756	None
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology (Reading, Engl.) 2007
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008
Interaction	Regulatory Rv1403c	priti.priety	IMP		authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007
Interaction	Regulatory Rv1403c	priti.priety	IMP		authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology (Reading, Engl.) 2007
Interaction	Regulatory Rv1403c	priti.priety	IMP		P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008
Interaction	Regulatory Rv1403c	ashwinigbhat	IMP		authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007
Interaction	RegulatedBy Rv3676	yamir.moreno	TAS		Literature previously reported link (from Balazsi et al. 2008). Traceable author statement to experimental support. G. Balzsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008

Property

Value

Creator

Evidence

PMID

Comment

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007

Citation

Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Microbiology (Reading, Engl.) 2007

ashwinigbhat

IMP

17906132

None

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

authors,P. Golby,KA. Hatch,J. Bacon,R. Cooney,P. Riley,J. Allnutt,J. Hinds,J. Nunez,PD. Marsh,RG. Hewinson,SV. Gordon Comparative transcriptomics reveals key gene expression differences between the human and bovine pathogens of the Mycobacterium tuberculosis complex. Microbiology (Reading, Engl.) 2007

Citation

Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. P. Golby, J. Nunez et al. Microbiology (Reading, Engl.) 2008

ashwinigbhat

IMP

18375799

None

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008

Interaction

Regulatory Rv1404

ashwinigbhat

IEP

qRT-PCR
P. Golby, J. Nunez et al. Characterization of two in vivo-expressed methyltransferases of the Mycobacterium tuberculosis complex: antigenicity and genetic regulation. Microbiology (Reading, Engl.) 2008

Citation

Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. authors,KH. Rohde,RB. Abramovitch,DG. Russell Cell Host Microbe 2007

priti.priety

IMP

18005756

None

Interaction

Regulatory Rv1403c

priti.priety

IMP

authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007

Citation

priti.priety

IMP

17906132

None

Interaction

Regulatory Rv1403c

priti.priety

IMP

Citation

priti.priety

IMP

18375799

None

Interaction

Regulatory Rv1403c

priti.priety

IMP

Citation

Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. authors,KH. Rohde,RB. Abramovitch,DG. Russell Cell Host Microbe 2007

ashwinigbhat

IMP

18005756

None

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

Interaction

Regulatory Rv1403c

priti.priety

IMP

authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007

Interaction

Regulatory Rv1403c

priti.priety

IMP

Interaction

Regulatory Rv1403c

priti.priety

IMP

Interaction

Regulatory Rv1403c

ashwinigbhat

IMP

authors,KH. Rohde,RB. Abramovitch,DG. Russell Mycobacterium tuberculosis invasion of macrophages: linking bacterial gene expression to environmental cues. Cell Host Microbe 2007

Interaction

RegulatedBy Rv3676

yamir.moreno

TAS

Literature previously reported link (from Balazsi et al. 2008). Traceable author statement to experimental support.
G. Balzsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008

Comments