TB Genome Annotation Portal

Rv1380 (pyrB)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: aspartate carbamoyltransferase
  • REFSEQ: aspartate carbamoyltransferase catalytic subunit
  • PATRIC: Aspartate carbamoyltransferase (EC 2.1.3.2)
  • TUBERCULIST: Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase)
  • NCBI: Probable aspartate carbamoyltransferase PyrB (ATCase) (aspartate transcarbamylase)
  • updated information (H37Rv4):
    • gene name: pyrB
    • function:
    • reference:
• Type: Not Target
• Start: 1553232
• End: 1554191
• Operon:


• Trans-membrane region:
  
• Role: I.F.2 - Pyrimidine ribonucleotide biosynthesis
• GO terms:
  • GO:0044205 - 'de novo' UMP biosynthetic process (Uniprot)
  • GO:0040007 - growth (Uniprot)
  • GO:0016743 - carboxyl- or carbamoyltransferase activity (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0016597 - amino acid binding (Uniprot)
  • GO:0009220 - pyrimidine ribonucleotide biosynthetic process (Uniprot)
  • GO:0006520 - cellular amino acid metabolic process (Uniprot)
  • GO:0006221 - pyrimidine nucleotide biosynthetic process (Uniprot)
  • GO:0006207 - 'de novo' pyrimidine nucleobase biosynthetic process (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005829 - cytosol (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0004070 - aspartate carbamoyltransferase activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
  • CAP[c] + ASP[c] -> PI[c] + CAASP[c]
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  6PNZ	38%	Staphylococcus aureus (strain COL)	The structure of the Aspartate Transcarbamoylase trimer from Staphylococcus aureus complexed with PALA at 2.27 Resolution.
  2AT2	37%	Bacillus subtilis	MOLECULAR STRUCTURE OF BACILLUS SUBTILIS ASPARTATE TRANSCARBAMOYLASE AT 3.0 ANGSTROMS RESOLUTION
  5G1P	37%	HOMO SAPIENS	Aspartate transcarbamoylase domain of human CAD bound to carbamoyl phosphate
  5G1O	37%	HOMO SAPIENS	Aspartate transcarbamoylase domain of human CAD in apo form
  5G1N	37%	HOMO SAPIENS	Aspartate transcarbamoylase domain of human CAD bound to PALA
  3R7L	37%	Bacillus subtilis	Crystal Structure of PALA-bound Aspartate Transcarbamoylase from Bacillus subtilis
  3R7F	37%	Bacillus subtilis	Crystal Structure of CP-bound Aspartate Transcarbamoylase from Bacillus subtilis
  3R7D	37%	Bacillus subtilis	Crystal Structure of Unliganded Aspartate Transcarbamoylase from Bacillus subtilis
  4BJH	35%	AQUIFEX AEOLICUS	Crystal Structure of the Aquifex Reactor Complex Formed by Dihydroorotase (H180A, H232A) with Dihydroorotate and Aspartate Transcarbamoylase with N-(phosphonacetyl)-L-aspartate (PALA)
  3D6N	35%	Aquifex aeolicus	Crystal Structure of Aquifex Dihydroorotase Activated by Aspartate Transcarbamoylase

• Links to additional information on pyrB:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = -5.581 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MTPRHLLTAADLSRDDATAILDDADRFAQALVGRDIKKLPTLRGRTVVTMFYENSTRTRVSFEVAGKWMSADVINVSAAGSSVGKGESLRDTALTLRAAG
ADALIIRHPASGAAHLLAQWTGAHNDGPAVINAGDGTHEHPTQALLDALTIRQRLGGIEGRRIVIVGDILHSRVARSNVMLLDTLGAEVVLVAPPTLLPV
GVTGWPATVSHDFDAELPAADAVLMLRVQAERMNGGFFPSVREYSVRYGLTERRQAMLPGHAVVLHPGPMVRGMEITSSVADSSQSAVLQQVSNGVQVRM
AVLFHVLVGAQDAGKEGAA

Additional Information