TB Genome Annotation Portal

Rv1317c (alkA)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: ada regulatory protein alkA
  • REFSEQ: bifunctional methylated-DNA--protein-cysteine methyltransferase/O-6-methylguanine-DNA transcription regulator
  • PATRIC: Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)
  • TUBERCULIST: Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase)
  • NCBI: Probable bifunctional regulatory protein and DNA repair enzyme AlkA (regulatory protein of adaptative response) (methylphosphotriester-DNA--protein-cysteine S-methyltransferase)
  • updated information (H37Rv4):
    • gene name: alkA
    • function:
    • reference:
• Type: Not Target
• Start: 1477628
• End: 1479118
• Operon:


• Trans-membrane region:
  
• Role: II.A.5 - DNA replication, repair, recombination and restriction/modification
• GO terms:
  • GO:0052822 - DNA-3-methylguanine glycosylase activity (Uniprot)
  • GO:0052821 - DNA-7-methyladenine glycosylase activity (Uniprot)
  • GO:0051409 - response to nitrosative stress (Uniprot)
  • GO:0046872 - metal ion binding (Uniprot)
  • GO:0043916 - DNA-7-methylguanine glycosylase activity (Uniprot)
  • GO:0043565 - sequence-specific DNA binding (Uniprot)
  • GO:0032993 - protein-DNA complex (Uniprot)
  • GO:0032259 - methylation (Uniprot)
  • GO:0032131 - alkylated DNA binding (Uniprot)
  • GO:0016787 - hydrolase activity (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0008725 - DNA-3-methyladenine glycosylase activity (Uniprot)
  • GO:0008270 - zinc ion binding (Uniprot)
  • GO:0008168 - methyltransferase activity (Uniprot)
  • GO:0008152 - metabolic process (Uniprot)
  • GO:0006974 - cellular response to DNA damage stimulus (Uniprot)
  • GO:0006355 - regulation of transcription, DNA-templated (Uniprot)
  • GO:0006351 - transcription, DNA-templated (Uniprot)
  • GO:0006307 - DNA dealkylation involved in DNA repair (Uniprot)
  • GO:0006285 - base-excision repair, AP site formation (Uniprot)
  • GO:0006284 - base-excision repair (Uniprot)
  • GO:0006281 - DNA repair (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0005618 - cell wall (Uniprot)
  • GO:0003905 - alkylbase DNA N-glycosylase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
  • GO:0003700 - DNA binding transcription factor activity (Uniprot)
  • GO:0003677 - DNA binding (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure:
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  1EYF	42%	Escherichia coli	REFINED STRUCTURE OF THE DNA METHYL PHOSPHOTRIESTER REPAIR DOMAIN OF E. COLI ADA
  1ADN	42%	Escherichia coli	SOLUTION STRUCTURE OF THE DNA METHYLPHOSPHOTRIESTER REPAIR DOMAIN OF ESCHERICHIA COLI ADA
  1ZGW	37%	Escherichia coli	NMR structure of E. Coli Ada protein in complex with DNA
  1U8B	37%	Escherichia coli	Crystal structure of the methylated N-ADA/DNA complex
  1WPK	35%	Escherichia coli	Methylated Form of N-terminal Transcriptional Regulator Domain of Escherichia Coli Ada Protein

• Links to additional information on alkA:
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 1.319 (from Jeremy Rock's lab)

Amino Acid Sequence

VHDDFERCYRAIQSKDARFDGWFVVAVLTTGVYCRPSCPVRPPFARNVRFLPTAAAAQGEGFRACKRCRPDASPGSPEWNVRSDVVARAMRLIADGTVDR
DGVSGLAAQLGYTIRQLERLLQAVVGAGPLALARAQRMQTARVLIETTNLPFGDVAFAAGFSSIRQFNDTVRLACDGTPTALRARAAARFESATASAGTV
SLRLPVRAPFAFEGVFGHLAATAVPGCEEVRDGAYRRTLRLPWGNGIVSLTPAPDHVRCLLVLDDFRDLMTATARCRRLLDLDADPEAIVEALGADPDLR
AVVGKAPGQRIPRTVDEAEFAVRAVLAQQVSTKAASTHAGRLVAAYGRPVHDRHGALTHTFPSIEQLAEIDPGHLAVPKARQRTINALVASLADKSLVLD
AGCDWQRARGQLLALPGVGPWTAEVIAMRGLGDPDAFPASDLGLRLAAKKLGLPAQRRALTVHSARWRPWRSYATQHLWTTLEHPVNQWPPQEKIA

Additional Information