TB Genome Annotation Portal

Rv1316c (ogt)

• Current annotations:
  • TBCAP: (community-based annotations - see table at bottom of page)
  • TBDB: methylated-DNA-protein-cysteine methyltransferase
  • REFSEQ: methylated-DNA--protein-cysteine methyltransferase
  • PATRIC: Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)
  • TUBERCULIST: Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase)
  • NCBI: Methylated-DNA--protein-cysteine methyltransferase Ogt (6-O-methylguanine-DNA methyltransferase) (O-6-methylguanine-DNA-alkyltransferase)
  • updated information (H37Rv4):
    • gene name: ogt
    • function:
    • reference:
• Type: Not Target
• Start: 1477134
• End: 1477631
• Operon:


• Trans-membrane region:
  
• Role: II.A.5 - DNA replication, repair, recombination and restriction/modification
• GO terms:
  • GO:0051409 - response to nitrosative stress (Uniprot)
  • GO:0032259 - methylation (Uniprot)
  • GO:0016740 - transferase activity (Uniprot)
  • GO:0008168 - methyltransferase activity (Uniprot)
  • GO:0006974 - cellular response to DNA damage stimulus (Uniprot)
  • GO:0006307 - DNA dealkylation involved in DNA repair (Uniprot)
  • GO:0006281 - DNA repair (Uniprot)
  • GO:0005886 - plasma membrane (Uniprot)
  • GO:0005737 - cytoplasm (Uniprot)
  • GO:0003908 - methylated-DNA-[protein]-cysteine S-methyltransferase activity (Uniprot)
  • GO:0003824 - catalytic activity (Uniprot)
• Reaction(s) (based on iSM810 metabolic model):
• Gene Expression Profile (Transcriptional Responses to Drugs; Boshoff et al, 2004)
• Gene Modules extracted from cluster analysis of 249 transcriptomic datasets using ICA
• Orthologs among selected mycobacteria
• Protein structure: 4bhc, 4bhb
• Search for Homologs in PDB
• Top 10 Homologs in PDB (as of Nov 2020):
  

  PDB	aa ident	species	PDB title
  4WX9	99%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis OGT in complex with DNA
  4BHB	99%	MYCOBACTERIUM TUBERCULOSIS	Crystal structure of Mycobacterium tuberculosis O6-METHYLGUANINE METHYLTRANSFERASE
  4WXD	98%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis OGT-R37K
  4WXC	98%	Mycobacterium tuberculosis	Crystal structure of Mycobacterium tuberculosis OGT-Y139F
  4BHC	98%	MYCOBACTERIUM TUBERCULOSIS	CRYSTAL STRUCTURE OF THE M. TUBERCULOSIS O6-METHYLGUANINE METHYLTRANSFERASE R37L VARIANT
  1SFE	47%	Escherichia coli	ADA O6-METHYLGUANINE-DNA METHYLTRANSFERASE FROM ESCHERICHIA COLI
  4HDV	38%	Schizosaccharomyces pombe	Crystal structure of S. pombe ATL1 in complex with damaged DNA containing 2,6-diaminopurine
  4HDU	38%	Schizosaccharomyces pombe	Crystal structure of S. pombe ATL1 in complex with damaged DNA containing 2-aminopurine
  4ENN	38%	Schizosaccharomyces pombe 972h-	Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-carboxymethylguanine
  4ENM	38%	Schizosaccharomyces pombe	Crystal structure of S. pombe Atl1 in complex with damaged DNA containing O6-benzylguanine

• Links to additional information on ogt:
  • TBDB (updated)
  • Phyre2 structure prediction, model: final.casp.pdb (from Mao et al, 2013)
  • UniProt
  • AlphaFold model
  • Vulnerability = 0.913 (from Jeremy Rock's lab)
  • KEGG - nucleotide and amino acid sequences of gene
  • Mycobrowser
  • PATRIC
  • BioCyc
  • Systems Biology

Amino Acid Sequence

MIHYRTIDSPIGPLTLAGHGSVLTNLRMLEQTYEPSRTHWTPDPGAFSGAVDQLNAYFAGELTEFDVELDLRGTDFQQRVWKALLTIPYGETRSYGEIAD
QIGAPGAARAVGLANGHNPIAIIVPCHRVIGASGKLTGYGGGINRKRALLELEKSRAPADLTLFD

Additional Information